StemCell Therapy

If you just started to study hematopoiesis you can always see in the protocols indication to so-called lineage-negative population in bone marrow.

Lineage-positive (Lin+) cells are a mix of all cells expressing mature cell lineage markers. For example for mouse bone marrow: Mac-1 for myeloid cells, CD4 and CD8 for T-cells, CD19 or B220 for B-cells, Ter-119 for erythrocytes, ect. The rest of the cells are lineage-negative (Lin-) they are not stained by the lineage antibodies. All stem and progenitor cell activity was identified withing Lin- population, but not in Lin+ cells.

Lin- populations are very heterogeneous and contain a small % of true stem cells, most of the cells are progenitors;

Lin Ab mix ready to use - mouse: BioLegend FITC or PE Anti-Mouse Lineage Cocktail With Isotype Control Caltag/Invitrogen Mouse Lineage Mixture, Hamster/Rat Anti-Mouse biotin / Alexa Fluor 488 human: eBioscience Human Hematopoietic Lineage Flow Cocktail (FITC)

kits for isolation Lin- population: mouse: Miltenyi Biotech -Lineage Cell Depletion Kit R&D Systems MagCellect Mouse Hematopoietic Cell Lineage Depletion Kit Stem Cell Technologies Customize your kit! for human and mouse human: Miltenyi Biotech -Lineage Cell Depletion Kit

biothinylated anti-mouse Ab: CD4, CD8, B220, IL-7R, Ter119, Gr-1, Mac1 from Adult mouse hematopoietic stem cells: purification and single-cell assays. Nature Protocols 2007; 1: 2979

******************** I have experience with commercial and hand-made customizable Lin Ab mixtures and kits. Please let us know if you have any trouble isolating a clean Lin- population, and wed love your feedback on products and kits youve used either successfully or unsuccessfully.

Tagged as: cell separation, FACS, flow cytometry, HSC isolation, lineage, MACS

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What is lineage-negative cells? - Stem Cell Assays

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