StemCell Therapy

StemFit is a xeno-free, defined medium proven to effectively maintain Induced Pluripotent Stem (iPS) and Embryonic Stem (ES) cells under feeder-free conditions during the reprogramming, expansion and differentiation phases of stem cell culture. Now available in research and clinical grade formulations StemFit combines market-leading colony forming efficiency with lower than standardmedia volume consumption to offer the most cost-effective colony expansion compared to leading competitors.

Benefits:

Find out about our new StemFit and iMatrix Recombinant Laminin Coating Free protocol.

Weekend-Free Cell Culture:

Figure 1. StemFit allows flexible weekend free protocols.

Greater Than x100 Folder Expansion:

Figure 2. Human 201B7 iPSCs grown on MEFs (feeder dependent)were transitioned to feeder-freeconditions with StemFit or commerciallyavailable medium A on respective ECMs (1000cells/cm2), and cultured for one week onpre-coated plates.

Reduced Media Consumption:

Figure 3. StemFit offers unparrallel cost savings, requiring less media volume and less media changes to achieve the same expansion.

Easy Transition from Feeder to Feeder-Free Culture:

Figure 4. Schematic of the transition of 201B7 iPSCs from feeder cell conditions to feeder free using StemFit. Protocol courtesy of the Center for iPSC Research and Application, Kyoto University.

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Superior Colony Forming Efficiency From a Single Clone:

StemFit culture medium has been independently evaluated to offer a high rate of colony expansion ofundifferentiated iPS/ES cells, while retaining normal karyotype following long-term passage.

Figure 5. Human iPSCs were adapted to StemFit,or commercially available medium A or B onMatrigel for more than 3 passages. Then,cells were serially diluted and seeded with eachmedium on Matrigel-coated 96-well platesat 1 cell/well or 10 cells/well. The number ofseeded cells was counted after 3 hours, andcolonies were counted at day 7.

Superior Performance of StemFit for the Culture of Induced Pluripotent Stem Cells. An independant study performed by CGT Catapult.

Figure 6. Compared against the 4 leading stem cell culture media (M2 - M5), StemFit (M1) demonstrates high rates of cell growth. Densities of approx. 7x105 viable cells/cm2 (vc/cm2) were achieved with protocol M1 after a 7-day culture cycle. Thenumber of doublings per day was found to be higher in M1

Highly Stable and Reproducible Feeder-Free Culture:

Figure 7. Human 201B7 iPSCs were cultured on iMatrix Laminin-511 with StemFit for 4 weeks without weekendfeeding. Cell colonies were dissociated into single cells and seeded at the listed densities.

Citations:

Ryuji Morizane & Joseph V Bonventre (2017) Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells. Nature Protocols 12, 195207 (2017).

Miyazaki, T. et al. (2017) Efficient Adhesion Culture of Human Pluripotent Stem Cells Using Laminin Fragments in an Uncoated Manner. Scientific Reports 7: 41165.

Camp, J. G. et al. (2017) Multilineage communication regulates human liver bud development from pluripotency. Nature 2017 Jun 22;546(7659):533-538.

Nakagawa, M. et al.(2014) A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells. Scientific Reports. 4, Article number: 3594.

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