DUBLIN--(BUSINESS WIRE)--
Research and Markets (http://www.researchandmarkets.com/research/fc9dd6/primary_and_stem_c) has announced the addition of John Wiley and Sons Ltd's new book "Primary and Stem Cells: Gene Transfer Technologies and Applications" to their offering.
This book describes basic cell engineering methods, emphasizing stem cell applications, and use of the genetically modified stem cells in cell therapy and drug discovery. Together, the chapters introduce and offer insights on new techniques for engineering of stem cells and the delivery of transgenes into stem cells via various viral and non-viral systems. The book offers a guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigator's purpose, whether it's basic science investigation, creation of disease models and screens, or cells for therapeutic applications.
Key Topics Covered:
PART I: CLONING AND GENE DELIVERY
1. DNA Assembly Technologies Based on Homologous Recombination
2. Multigene Assembly for Construction of Synthetic Operons: Creation and Delivery of an Optimized All-IN-One Expression Construct for Generating Mouse iPS Cells
3. Strategies for the Delivery of Naked DNA
PART II: NONINTEGRATING TECHNOLOGIES
4. Episomal Vectors
5. Nonintegrating DNA Virus
6. Nonintegrating RNA Viruses
7. Protein Delivery
PART III: INTEGRATING TECHNOLOGIES
8. Sleeping Beauty Transposon-Mediated Stable Gene Delivery
9. Integrating Viral Vectors for Gene Modifications
10. Bacteriophage Integrases for Site-Specific Integration
11. Improving Gene Targeting Efficiency in Human Pluripotent Stem Cells
PART IV: APPLICATIONS
12. Modified Stem Cells as Disease Models and in Toxicology Screening
13. Screening and Drug Discovery
INDEX
Author:
UMA LAKSHMIPATHY is a principal investigator at Life Technologies. She has a PhD in life sciences, with academic and industry experience in molecular biology and stem cells. Dr. Lakshmipathy holds four patents and has authored more than forty publications.
BHASKAR THYAGARAJAN is a program manager at Life Technologies. He has a PhD in pharmacology, with expertise in the areas of molecular biology, DNA recombination, gene and cell therapy, and protein purification. He holds one patent and has authored more than twenty publications.
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