StemCell Therapy

It turns out that not all the hairlike cilia projecting from the surfaces of many cells in the human body are equal--there are the myriad ones for sweeping, swimming and other functions, and then there is the until recently mysterious primary cilium.

Nearly all human cells contain these numerous microscopic projections. The more abundant variety of cilia are motile; they act like oars, paddling in coordinated waves to help propel cells through fluid, or to sweep material across cellular surfaces (as in the respiratory system, where millions of cilia lining the airways help to expel mucus, dead cells and other bodily debris). By contrast, cells also contain a single, nonmotile cilium known as the primary cilium. Its presence on cells has been known for more than a century, but many believed it was a functionless evolutionary remnant.

[More]

Cell - Biology - Cell biology - Cilium - Human body

Arthritis Patient Successfully Treated With Fat Stem Cells Tells His Story

SAN DIEGO, CA--(Marketwire - June 28, 2010) - Medistem Inc. (PINKSHEETS: MEDS). Medistem collaborator Dr. Jorge Paz Rodriquez was invited to give a talk at Del Mar College in Texas by arthritis patient Dusty Durrill. The patient described a profound recovery after treatment with stem cells from his own fat tissue. Mr Durrill underwent a procedure in which a small amount of fat tissue was extracted by liposuction, stem cells where purified, and subsequently injected intravenously.

This procedure has been used successfully to treat thousands of animals suffering from arthritis in the United States (www.vet-stem.com). Use of patient's own stem cells is currently being performed in the United States (www.regenexx.com). Recently Dr. Paz published a paper describing scientific mechanisms of this treatment in collaboration with scientists from the University of California San Diego, University of Western Ontario, and Medistem Inc (Ichim et al. Autologous stromal vascular fraction cells: A tool for facilitating tolerance in rheumatic disease. Cell Immunol. 2010 Apr 8).

"I had treatment for my arthritis, I was not wheelchair bound but I was getting there... after stem cell treatment my arthritis symptoms disappeared," stated Mr. Durrill.

More than 200 people attended the lecture including the general public, patients and medical doctors. The lecture was focused on US and European clinical trials supporting the use of adult stem cells in conditions ranging from multiple sclerosis, to heart failure, to diabetes. A video of part of the lecture is available at http://www.kiiitv.com/younews/97165699.html.

Dr. Paz commented, "Mr. Durrill suffered from arthritis for more than ten years with severe pain in both knees and hips. He had difficulty standing and limited mobility. After stem cell therapy he started showing significant reduction in pain. Now about a month after therapy he is pain free and can move around easily."

Drs. Robert Harman, CEO of Vet-Stem and Thomas Ichim, CEO of Medistem, recently released a video discussing their publication on fat stem cell therapy for arthritis. The video is available at http://www.youtube.com/watch?v=3QQrwtp-KQQ.

About Medistem Inc.

Medistem Inc. is a biotechnology company developing technologies related to adult stem cell extraction, manipulation, and use for treating inflammatory and degenerative diseases. The company's lead product, the endometrial regenerative cell (ERC), is a "universal donor" stem cell being developed for critical limb ischemia. A publication describing the support for use of ERC for this condition may be found at http://www.translational-medicine.com/content/pdf/1479-5876-6-45.pdf

Cautionary Statement

This press release does not constitute an offer to sell or a solicitation of an offer to buy any of our securities. This press release may contain certain forward-looking statements within the meaning of Section 27A of the Securities Act of 1933, as amended, and Section 21E of the Securities Exchange Act of 1934, as amended. Forward-looking statements are inherently subject to risks and uncertainties, some of which cannot be predicted or quantified. Future events and actual results could differ materially from those set forth in, contemplated by, or underlying the forward-looking information. Factors which may cause actual results to differ from our forward-looking statements are discussed in our Form 10-K for the year ended December 31, 2007 as filed with the Securities and Exchange Commission.

Scientists grow a rat lung in the laboratory

By LAURAN NEERGAARD (AP) – 4 days ago

WASHINGTON — It's an early step toward one day building new lungs: Yale University researchers took apart and regrew a rat's lung, and then transplanted it and watched it breathe.

The lung stayed in place only for an hour or two, as the scientists measured it exchanging oxygen and carbon dioxide much like a regular lung — but also spotted some problems that will take more research to fix.

Still, the work is a step in the science fiction-sounding hunt for ways to regenerate damaged lungs — although lead researcher Dr. Laura Niklason cautions that it may be 20 or 25 years before a build-a-new-organ approach is ready for people.

The work was reported online Thursday in the journal Science.

Nearly 400,000 people die of lung diseases each year in the U.S. alone, according to the American Lung Association, and lung transplants are far too rare to offer much help.

But how to replicate these spongy organs? Niklason's team stripped an adult rat's lung down to its basic structural support system — its scaffolding — to see if it's possible to rebuild rather than starting completely from scratch.

First, they essentially washed away the different kinds of cells lining that lung. It gradually faded from a healthy red to a white structure of mostly collagen and other connective tissue that maintained the shape and stretchiness of the original lung, even the tubes where airways would be.

This scaffolding is like a universal donor that shouldn't pose rejection problems, said Niklason: "Your collagen and my collagen are identical."

The researchers put the lung scaffolding into a bioreactor, an incubator-style container designed to mimic the environment in which fetal lungs develop, with fluid pumping through them.

Then they injected a mixture of different lung cells taken from a newborn rat. In the bioreactor, those cells somehow migrated to the right spots and grew air sacs, airways and blood vessels.

In short-term implants in four different rats, engineered lungs replaced one of the animals' native lungs and proved 95 percent as efficient at exchanging oxygen and carbon dioxide, Niklason said.

However, among the problems she spotted were small clots that formed inside the engineered lung, a sign that the new cells hadn't grown a thick enough cover in some places.

The biggest challenge: For this approach ever to work without a person's body rejecting the new tissue, scientists would need to use a recipient's own cells, Niklason explained. But there isn't a way yet to cull the kind of personalized stem cells that would be needed, meaning stem cell research must improve first, she said.

This overall approach also worked in a 2008 University of Minnesota experiment that grew a beating rat heart, and Minnesota researcher Dr. Doris Taylor welcomed the Yale lung work.

Separately in Science, a Harvard University team coated a flexible chip with layers of living lung cells, creating a laboratory tool that mimics some of the action of a breathing human lung. The goal: To replace some of the animal studies needed to test how lungs react to environmental toxins or inhaled drugs.

Online: http://www.sciencemag.org

Doctors are saying stem cell transplants are a promising new treatment to restore sight to individuals who have suffered severe eye damage. Dozens of patients whose eyes were injured after being splashed with caustic chemicals were able to see again after receiving a transplant of their own stem cells, according to The Associated Press.

Italian researchers reported that the transplants had worked completely in 82 of 107 eyes, including in one patient who sustained severe eye injuries some 60 years ago and has had his sight almost completely restored. The transplant worked at least partially in 14 other eyes, and the benefits have lasted for up to 10 years.

"This is great work, an absolutely great way to do it," said Dr. Douglas Lazzaro, chairman of ophthalmology at Long Island College Hospital. "It can only increase the success rate of these types of procedures."

Dr. Bruce Rosenthal, chief of low-vision programs at Lighthouse International, a nonprofit that fights vision impairment, called the stem cell transplants a very promising treatment. The procedure bypasses the risk of rejection posed by corneal transplants because the patient’s own stem cells are used.

"This is a major step in returning vision to someone who has lost it," Rosenthal says.

University of California ophthalmologist Ivan Schwab, who is not involved in the study, called the research "a roaring success."

Each year, stem cell transplants could offer the promise of healing to thousands of people who sustain chemical burns on their corneas, although they would not help those with macular degeneration, which involves the eye’s retina, or those with damage to the optic nerve. People who are blind in both eyes also would not be candidates for the transplant because some healthy tissue is required to undergo it, doctors explained.

The researchers who performed the transplants for the study, which was published online by the New England Journal of Medicine, removed stem cells from the patient’s good eye, grew them in the lab and put them back in the injured eye. There, they grew new corneal tissue that replaced the damaged tissue. None of the patients needed anti-rejection drugs.

For many years, adult stem cells have been used to treat disorders as varied as leukemia and sickle cell anemia. But fixing damaged eyes with a stem cell transplant is relatively new - and so far is not being done here.

"The U.S. is pretty stringent," Rosenthal says. "They won’t allow these procedures until they are FDA-approved and have gone through a lot of clinical testing. But even though it’s not ready for prime time, there is a lot of hope for the future."

Some 61 million Americans are at high risk for serious vision loss, according to Lighthouse International, and about 61 million individuals age 45 and older will have vision loss by 2015. A Lighthouse survey revealed that fewer than half (46%) of Americans get an annual eye exam.

WITH NEWS WIRE SERVICES

Read more: http://www.nydailynews.com

(Reuters) - A U.S. appeals court on Friday reinstated a lawsuit that challenges an Obama administration policy for federal funding of some human embryonic stem cell research.

The unusual suit against the National Institutes of Health, backed by some Christian groups opposed to embryo research, argued that the NIH policy takes funds from researchers seeking to work with adult stem cells.

It also argues that new Obama administration guidelines on stem cell research are illegal.

The three-judge federal appeals panel did not rule on the merits of the lawsuit itself, but said two of the doctors involved had legal standing to file it.

A federal district court had earlier rejected the lawsuit, saying the challengers had no standing.

Stem cells are the body's master cells. There are several kinds, including those taken from days-old human embryos, which can give rise to all the cells and tissues in the body.

Some people oppose working with human embryonic stem cells, but President Barack Obama's administration reversed a policy that severely limited federal funding of such work.

The NIH will now pay for research using the cells, although it will not pay for the actual process of making the cells, which does involve human embryos. The use of federal funds to pay for the destruction of human embryos is forbidden by law.

The NIH also funds work with so-called adult stem cells, immature cells found throughout the body.

Dr. James Sherley, a biological engineer at Boston Biomedical Research Institute who opposes the use of embryonic stem cells, had argued that the guidelines violated the law by permitting research on stem cells derived from human embryos and would harm their work by increasing competition for limited federal funding.

Sherley and Theresa Deisher of Washington-based AVM Biotechnology were joined in their challenge by the Christian Medical Association, which opposes federal funding of embryonic stem cell research, and an adoption agency called Nightlight Christian Adoptions, which had argued that the guidelines reduced the number of embryos available for use in adoption.

For those of you who like stories with simple plots and tidy endings, I must confess the tale of the Human Genome Project isn't one of those. The story didn't reach its conclusion when we unveiled the first draft of the human genetic blueprint at the White House on June 26, 2000. Nor did it end on April 14, 2003, with the completion of a finished, reference sequence. [More]

Human Genome Project - White House - Biology - genetic - Francis Collins

Jeffrey Janus serves as director and senior vice president of operations of new CHI member International Stem Cell Corp. and president and chief executive officer of Lifeline Cell Technology, one of the company’s subsidiaries. International Stem Cell Corp. (ISCO.OB) is a publicly traded stem cell therapy company with research and manufacturing facilities in Oceanside, Calif., and Walkersville, Md. The company’s technology revolves around its discovery of a proprietary and unique class of stem cells called human parthenogenetic stem cells (hpSC). These cells have distinct medical, practical and ethical advantages over embryonic and adult stem cells. They allow immune-matched stem cells and therapeutic cells to be “banked” and available immediately for millions of patients who are in critical need and cannot wait to derive cells from their own bodies. In addition to Lifeline Cell Technology, the company has another subsidiary called Lifeline Skin Care.

Janus is trained in biochemistry and business management and has more than 20 years experience focused on cell-based businesses. He is a member of the team that discovered parthenogenesis and is published in the stem cell field. After joining International Stem Cell Corp., (ISCO) Janus subsequently founded Lifeline Cell Technology to meet a growing need for media and human cells in pharmaceutical drug screening, consumer product testing and basic research at universities and government laboratories and to provide revenue and operational infrastructure for ISCO. The CHI Blog recently caught up with Janus to find out the latest on the company.

Q: How did your company get started? A: We started this company based on the work of Elena Revazova, M.D., Ph.D., a scientist well known in Russia who had a dream of curing diabetes using embryonic stem cells. She came to the United States to work and her talent and expertise in growing human cells was discovered by ISCO’s founders, who decided to form a company around her knowledge and skill. At the time, U.S. President [George W.] Bush was restricting the use of embryonic stem cells on ethical grounds, and there were also patent issues around embryonic stem cells, as there still are. We recognized that the ethical issue was important, but medially the most important problem with stem cell therapy was likely to be immune rejection. We realized we could address these issues by developing the technology called parthenogenesis and mitigate delays from funding and restrictions by working in Russia. So Dr. Revazova went back to Russia, and we set up a collaboration in Moscow to begin her work with parthenogenesis. Today our company has all of the intellectual property rights to parthenogenesis, a very powerful technology. We have also recently brought in Andrey Semechkin, Ph.D. as our CEO. Dr. Semechkin is a well-known scientist in the field of systems analysis and an accomplished businessman.

Q: How does parthenogenesis work? A: It’s the derivation of stem cells from an unfertilized human egg. The ethical issue surrounding work with embryonic stem cells is caused by the fact that embryonic stem cells are derived from a fertilized embryo, which has the potential to be a human being. However, if you do not fertilize the egg and yet you can derive stem cells from it that are functional, you’re not destroying a viable human embryo—and that’s exactly what Dr. Revazova did. We perfected parthenogenesis and brought it back to the United States. As a result, we have been able to overcome the ethical issue surrounding using embryonic stem cells with parthenogenesis.

Q: What are your technology’s other advantages? A: Parthenogenesis makes embryonic stem cells (or what we call parthenogenetic stem cells) that can be immune matched to millions of people. Using embryonic stem cells, the way they are currently made, is sort of like trying to do a bone marrow transplant between one person and another picked at random without making sure you have a match. If someone needs to have a bone marrow transplant, they usually go to brothers or sisters first and try to do an immune match. For a different set of reasons a similar situation exists with blood transfusions, although type O blood can be given to almost everyone. Our cells are similar in that the parthenogenic stem cells can be immune matched to many people, and that’s the unique quality of our cells.

Q: What are the biggest opportunities for your business going forward? A: We are creating a bank of hpSC that are “pluripotent” and carry common immune types that will match a large percent of the U.S. population, and this is a huge opportunity. These will be clinical grade and will be made in our new manufacturing facility located in Oceanside, Calif. Our biggest opportunity is the potential ability of our stem cells to be universally utilized for therapy. Scientists across the world are working on embryonic stem cells and figuring out ways to make therapeutic cells such as liver cells or nerve cells for a whole host of diseases. Eventually these therapies will need a cell or process that will minimize immune rejection. Our cells can be immune matched to millions of persons and are thus a solution for this need. So in a way, much of the work that’s going on right now across the world with embryonic stem cells accrues to our benefit. In addition, we are focused in four distinct areas—diabetes, liver disease, retinal and corneal disease, and nerve disease. We are currently growing cells to cure corneal blindness and have actually grown cornea tissue. We’re working with the University of California, Irvine to grow cells with a retina for macular degeneration. We have grown cells that are very similar to liver cells that are also related to a cell type called beta cells, which may be useful for diabetes. Collaborations with companies and universities present strong opportunities, and we’ve collaborated with Novocell in San Diego to further our work with diabetes, and we’re collaborating with UC San Francisco to test our liver cells derived from our parthenogenic stem cells and with researchers in Germany to study nerve cells generated from our stem cells.

Q: Tell us a little bit about your subsidiaries. A: One unique thing about our company is that we are a research-oriented biotech company that actually has income. One of our subsidiaries, Lifeline Cell Technology, is growing very nicely (with a 150 percent increase in sales over the last year) by selling research products to grow human cells and study human disease. Lifeline has more than 70 products and will be releasing more than a dozen more in 2010. Lifeline Skin Care was created in 2009 based on our discovery that derivatives from our parthenogenetic stem cell technology have proven to be beneficial to human skin. Lifeline Skin Care is developing several products and is beginning early-stage clinical trials with these skin products. We anticipate that these skin care products will help to generate income and fund our continuing stem cell therapeutic research.

Q: What are your company’s greatest accomplishments so far? A. We have successfully created 10 human parthenogenetic stem cell lines, one which carries the most common immune type in the United States and matches over a hundred million persons across the world. We are a fast-growing company with more than 12 scientists working in various areas of therapy and product development. Our stem cells have proven to be able to create cells that may be useful in therapy, including liver-like cells, corneal cells, retinal cells, nerve cells and cell types that may ultimately be useful in the treatment of diabetes. We have set up collaborations with major universities and researchers across the world. The amazing thing about our company is that we have developed into a company that has manufacturing, products, sales, quality control, therapeutic research, and an accounting department in such a short time. We have all the workings of a fully functional product manufacturing and therapeutic research company. It amazes me that we are making sales, whereas most companies our size are basic research and development companies. We know how to make human cells and freeze, store and manipulate them so that they are clinical grade. I think our technology, our knowledge of cell culture and our ability to manufacture are three very strong reasons that we have been successful.

CHI-Advancing California biomedical research and innovation
SOURCE: http://californiahealthcareinstitute.blogspot.com/2010/02/executive-spotlight-jeffrey-janus.html

For those of you who like stories with simple plots and tidy endings, I must confess the tale of the Human Genome Project isn't one of those. The story didn't reach its conclusion when we unveiled the first draft of the human genetic blueprint at the White House on June 26, 2000. Nor did it end on April 14, 2003, with the completion of a finished, reference sequence. [More]

Human Genome Project - White House - Biology - genetic - Francis Collins

Jeffrey Janus serves as director and senior vice president of operations of new CHI member International Stem Cell Corp. and president and chief executive officer of Lifeline Cell Technology, one of the company’s subsidiaries. International Stem Cell Corp. (ISCO.OB) is a publicly traded stem cell therapy company with research and manufacturing facilities in Oceanside, Calif., and Walkersville, Md. The company’s technology revolves around its discovery of a proprietary and unique class of stem cells called human parthenogenetic stem cells (hpSC). These cells have distinct medical, practical and ethical advantages over embryonic and adult stem cells. They allow immune-matched stem cells and therapeutic cells to be “banked” and available immediately for millions of patients who are in critical need and cannot wait to derive cells from their own bodies. In addition to Lifeline Cell Technology, the company has another subsidiary called Lifeline Skin Care.

Janus is trained in biochemistry and business management and has more than 20 years experience focused on cell-based businesses. He is a member of the team that discovered parthenogenesis and is published in the stem cell field. After joining International Stem Cell Corp., (ISCO) Janus subsequently founded Lifeline Cell Technology to meet a growing need for media and human cells in pharmaceutical drug screening, consumer product testing and basic research at universities and government laboratories and to provide revenue and operational infrastructure for ISCO. The CHI Blog recently caught up with Janus to find out the latest on the company.

Q: How did your company get started? A: We started this company based on the work of Elena Revazova, M.D., Ph.D., a scientist well known in Russia who had a dream of curing diabetes using embryonic stem cells. She came to the United States to work and her talent and expertise in growing human cells was discovered by ISCO’s founders, who decided to form a company around her knowledge and skill. At the time, U.S. President [George W.] Bush was restricting the use of embryonic stem cells on ethical grounds, and there were also patent issues around embryonic stem cells, as there still are. We recognized that the ethical issue was important, but medially the most important problem with stem cell therapy was likely to be immune rejection. We realized we could address these issues by developing the technology called parthenogenesis and mitigate delays from funding and restrictions by working in Russia. So Dr. Revazova went back to Russia, and we set up a collaboration in Moscow to begin her work with parthenogenesis. Today our company has all of the intellectual property rights to parthenogenesis, a very powerful technology. We have also recently brought in Andrey Semechkin, Ph.D. as our CEO. Dr. Semechkin is a well-known scientist in the field of systems analysis and an accomplished businessman.

Q: How does parthenogenesis work? A: It’s the derivation of stem cells from an unfertilized human egg. The ethical issue surrounding work with embryonic stem cells is caused by the fact that embryonic stem cells are derived from a fertilized embryo, which has the potential to be a human being. However, if you do not fertilize the egg and yet you can derive stem cells from it that are functional, you’re not destroying a viable human embryo—and that’s exactly what Dr. Revazova did. We perfected parthenogenesis and brought it back to the United States. As a result, we have been able to overcome the ethical issue surrounding using embryonic stem cells with parthenogenesis.

Q: What are your technology’s other advantages? A: Parthenogenesis makes embryonic stem cells (or what we call parthenogenetic stem cells) that can be immune matched to millions of people. Using embryonic stem cells, the way they are currently made, is sort of like trying to do a bone marrow transplant between one person and another picked at random without making sure you have a match. If someone needs to have a bone marrow transplant, they usually go to brothers or sisters first and try to do an immune match. For a different set of reasons a similar situation exists with blood transfusions, although type O blood can be given to almost everyone. Our cells are similar in that the parthenogenic stem cells can be immune matched to many people, and that’s the unique quality of our cells.

Q: What are the biggest opportunities for your business going forward? A: We are creating a bank of hpSC that are “pluripotent” and carry common immune types that will match a large percent of the U.S. population, and this is a huge opportunity. These will be clinical grade and will be made in our new manufacturing facility located in Oceanside, Calif. Our biggest opportunity is the potential ability of our stem cells to be universally utilized for therapy. Scientists across the world are working on embryonic stem cells and figuring out ways to make therapeutic cells such as liver cells or nerve cells for a whole host of diseases. Eventually these therapies will need a cell or process that will minimize immune rejection. Our cells can be immune matched to millions of persons and are thus a solution for this need. So in a way, much of the work that’s going on right now across the world with embryonic stem cells accrues to our benefit. In addition, we are focused in four distinct areas—diabetes, liver disease, retinal and corneal disease, and nerve disease. We are currently growing cells to cure corneal blindness and have actually grown cornea tissue. We’re working with the University of California, Irvine to grow cells with a retina for macular degeneration. We have grown cells that are very similar to liver cells that are also related to a cell type called beta cells, which may be useful for diabetes. Collaborations with companies and universities present strong opportunities, and we’ve collaborated with Novocell in San Diego to further our work with diabetes, and we’re collaborating with UC San Francisco to test our liver cells derived from our parthenogenic stem cells and with researchers in Germany to study nerve cells generated from our stem cells.

Q: Tell us a little bit about your subsidiaries. A: One unique thing about our company is that we are a research-oriented biotech company that actually has income. One of our subsidiaries, Lifeline Cell Technology, is growing very nicely (with a 150 percent increase in sales over the last year) by selling research products to grow human cells and study human disease. Lifeline has more than 70 products and will be releasing more than a dozen more in 2010. Lifeline Skin Care was created in 2009 based on our discovery that derivatives from our parthenogenetic stem cell technology have proven to be beneficial to human skin. Lifeline Skin Care is developing several products and is beginning early-stage clinical trials with these skin products. We anticipate that these skin care products will help to generate income and fund our continuing stem cell therapeutic research.

Q: What are your company’s greatest accomplishments so far? A. We have successfully created 10 human parthenogenetic stem cell lines, one which carries the most common immune type in the United States and matches over a hundred million persons across the world. We are a fast-growing company with more than 12 scientists working in various areas of therapy and product development. Our stem cells have proven to be able to create cells that may be useful in therapy, including liver-like cells, corneal cells, retinal cells, nerve cells and cell types that may ultimately be useful in the treatment of diabetes. We have set up collaborations with major universities and researchers across the world. The amazing thing about our company is that we have developed into a company that has manufacturing, products, sales, quality control, therapeutic research, and an accounting department in such a short time. We have all the workings of a fully functional product manufacturing and therapeutic research company. It amazes me that we are making sales, whereas most companies our size are basic research and development companies. We know how to make human cells and freeze, store and manipulate them so that they are clinical grade. I think our technology, our knowledge of cell culture and our ability to manufacture are three very strong reasons that we have been successful.

CHI-Advancing California biomedical research and innovation
SOURCE: http://californiahealthcareinstitute.blogspot.com/2010/02/executive-spotlight-jeffrey-janus.html

The first discovery of a cancer gene marker--the BRAF oncogene for melanoma and colorectal malignancies--back in 2002 changed the way many researchers thought about cancer treatment. Rather than approach the disease based on what region of the body it stemmed from, scientists began to identify cancers in terms of their genetic signatures. Researchers now recognize more than 200 kinds of cancer--all genetically unique. [More]

Cancer - Health - Conditions and Diseases - Colorectal cancer - Management of cancer

The first discovery of a cancer gene marker--the BRAF oncogene for melanoma and colorectal malignancies--back in 2002 changed the way many researchers thought about cancer treatment. Rather than approach the disease based on what region of the body it stemmed from, scientists began to identify cancers in terms of their genetic signatures. Researchers now recognize more than 200 kinds of cancer--all genetically unique. [More]

Cancer - Health - Conditions and Diseases - Colorectal cancer - Management of cancer

Cellonis Diabetes Stem Cell Therapy: A Chance for Insulin Independence and the Reversal of Complications

BEIJING, June 24 /PRNewswire-Asia/ -- Cellonis, a Beijing and HK-based biotech company, with its new personalized diabetes treatment concept has demonstrated an amazing improvement in their treated patients' conditions. The ongoing clinical study shows the treatment's best case could reconstruct a patient's natural insulin production and even reverse later complications like kidney failure. Treated patients may have the chance to return to the normal activities non-diabetes sufferers take for granted.

The clinical study, jointly conducted by scientists and doctors of the Cellonis clinical research team, aims to help patients be free from insulin and oral drugs, by reconstructing their natural insulin production damaged by either autoimmune disorder (T1DM) or hyperglycemia and also improve insulin sensitivity (T2DM). Using injections of autologous stem cells from the patient's bone marrow, the research team believes that this therapy could help patients return to a life without annoying everyday injections and drugs.

"Most of the patients in our clinical study are now taking less synthetic insulin or oral drugs for BG control," says Dr. Chase Dai, Chief Medical Officer at Cellonis. "We appear to have restored the biological insulin producing function of the body. We are excited to see that some patients have been treatment-free for five months now, and we believe the effect of the stem cell therapy can last much longer. We were also encouraged by some other happy surprises during the clinical study.

"For example, this therapy appears to reverse chronic kidney failure. It was a surprise for all of my team to observe that the kidney function of a 75- year-old patient improved remarkably."

This patient had suffered from diabetes for years, gradually developing diabetic foot and nephropathy. He can now walk freely after having been confined to bed or a wheelchair for six months, and his quality of life has improved significantly. Moreover, he only needs kidney dialysis one time a week instead of three times. In a follow-up visit he excitedly told us that he was hopeful that in the near future he could be completely rid of diabetes.

Diabetes, an increasingly spreading disease, can lead to life-threatening diseases such as blindness, amputation, strokes, or kidney failure in its natural course. Current treatments, including insulin, cannot change this situation.

"We believe that our stem cell therapy will bring promising hope for patients suffering from diabetes and its complications," comments Cindy Hao, CEO of Cellonis. "Personalized diabetes therapies for patients of various conditions will be developed by Cellonis in the near future. We believe what we have restored for patients will not only be their natural insulin production, but also a normal life filled with the activities non-diabetes sufferers can enjoy daily."

Cellonis Biotechnologies focuses on R&D and the clinical application of novel personalized stem cell therapies and immunotherapies for patients with diseases including cancer, diabetes and central nervous system disorders.

At three years old, Justin, a German Shepherd-cross, seems too young to be afflicted with osteoarthritis.

But his early life, marred by abuse, left Justin with arthritis, hip dysplasia, flesh-eating disease and a cracked molar. “He is a big mess. A lot of people would euthanize him, but I don’t want to give up on him,” said Jamie Lee, a Vancouverite who adopted Justin nine months ago from an animal rescue group.

The mission to relieve Justin’s pain brought Ms. Lee to the frontier of veterinary medicine: stem-cell therapy.

It’s one of the holistic practices, including raw food, acupuncture and massage, showcased at The Petnership Project Tradeshow and Lecture Series in Vancouver on Saturday.

Justin broke one of his legs as a puppy, but his owners never took him to the vet. The injury resulted in osteoarthritis that spread to his other limbs.

The specialist said they could try an implant to relieve the pain, but there was a high risk that failure could lead to amputation, Ms. Lee said.

Instead, Ms. Lee decided to try an innovative treatment called stem-cell therapy, a procedure that extracts cells from the animal’s fat and moves them into the injured area to jump-start healing.

The procedure took less than 48 hours, even though the fat was shipped to a San Diego-based company called Vet-Stem. The company extracts cells from the fat tissue and sends back a needle to be inserted into the pet’s source of pain.

Ms. Lee said Justin’s mobility has gradually increased by 50 per cent after the surgery. Now the pooch, who could barely walk, can stroll for an easy 30 minutes.

But pain relief doesn’t come cheap: Ms. Lee spent around $4,000 to get treatment for all four of Justin’s joints.

The average treatment cost runs between $1,200 and $1,500, said Loridawn Fawcett of Vancouver’s The Healing Place, who advised Justin’s treatment. The treatment only has to be done once, unless there is a new injury, she said.

Results can take up to three months, said Dr. Fawcett, adding the success rate is 80 per cent.

Most dogs have the therapy to treat arthritis, but it can help heal fractures, tendon-ligament injuries and liver disease, said Dr. Fawcett.

This therapy goes beyond stem-cell treatments available for humans in Canada, which require adult stem cells to be taken from a donor’s bone marrow. Research into whether humans can successfully harvest and use their own stem cells for regenerative therapy is ongoing, but is not yet approved.

While it may be exciting, Thomas Koch of the Ontario Veterinary College cautions the science behind the therapy is not proven. The cells may have a therapeutic effect, he said, but scientists don’t know exactly how or why.

“The marketing is trumping the science and it’s obviously feeding off the hope and hype in the whole area of regenerative medicine,” said Dr. Koch. Still, the procedure seems safe in terms of infection because patients are receiving their own cells, he said, adding that there is still a risk people are paying for ineffective therapy.

It was worth it for Justin, said Ms. Lee.

She advises other pet owners considering the option to do some research, get a full blood test and consult with their vet about whether stem cell therapy is the right choice for their pet.

“If something goes wrong in surgery, you cannot go back,” said Ms. Lee. “With stem-cell therapy, what’s the worst that can happen? You pay more money.”

(06-18) 13:42 PDT SAN FRANCISCO -- Dr. Shinya Yamanaka, a stem cell researcher at UCSF's Gladstone Institutes who discovered a technique for transforming adult skin cells into "pluripotent" stem cells without resorting to human embryos, has won Japan's $550,000 Kyoto Prize, an international award that honors scientific, cultural and spiritual contributions to human knowledge.

His discovery resulted in a class of much-sought stem cells that scientists can induce to become virtually any other type of functioning human cell that one day might be used to treat varied diseases or injuries.

During their research, Yamanaka and his colleagues altered the genetics of adult skin cells by inserting four specific viral genes that produce proteins known as transcription factors into the cells. Those proteins in turn yielded other genes that reprogrammed the skin cells so they acquired all the characteristics that made them what are now known as induced pluripotent cells.

Before his discovery, those pluripotent human stem cells could only be harvested from human embryos, a source posing such powerful ethical issues that former President George W. Bush banned virtually all embryonic stem cell research eight years ago. The ban remained in force until President Obama reversed it last year.

Yamanaka, 47, who is attending the annual meeting of the International Society of Stem Cell Research in San Francisco this week, was not told of his award until just before midnight Pacific time on Thursday.

The Kyoto Prize is awarded annually by Japan's Inamori Foundation for major discoveries in many fields of advanced technology, and four other Bay Area scientists have won it in recent years.

They are Leonard Herzenberg, a Stanford geneticist and immunologist who developed a revolutionary cell-sorting machine now crucial to advanced biomedical research; Alan Kay, a Silicon Valley pioneer at Hewlett-Packard who led advanced computing technology for 40 years; Donald E. Knuth, a Stanford information technology expert and specialist in computer programming, language analysis and computerized printing and Richard Karp, a UC Berkeley computer scientist and pioneer in computational biology.

In addition to heading his laboratory at the Gladstone Institute for Cardiovascular Research on the Mission Bay campus of UCSF, Yamanaka is also a professor at Kyoto University, where he began his efforts seeking a way of transforming adult cells.

Besides resolving ethical issues by his achievement, Yamanaka's success also means that the pluripotent stem cells needed to treat a patient's disease can be obtained from the ordinary skin cells of a patient's own body - thus making stem cell therapy possible without the hazards involved in immunologic rejection of cells from other people.

Robert Lanza, chief scientific officer of Advanced Cell Technology and an adjunct professor at Wake Forest University's stem cell research center, said recently that Yamanaka's work "is likely to be the most important stem cell breakthrough of all time."

"The ability to generate an unlimited supply of patient-specific stem cells will revolutionize the future of medicine," Lanza said.

Read more: http://www.sfgate.com

Unproven treatment results in mysterious masses.

David Cyranoski

In a stark reminder that stem-cell therapy is uncharted territory, a stem-cell transplant given to a patient in Thailand who had kidney disease resulted in the development of cellular masses not previously reported. The lesions, described in a paper published online on 17 June in the Journal of the American Society of Nephrology, were not directly linked to the patient's subsequent death (D. Thirabanjasak et al. J. Am. Soc. Nephrol. doi:10.1681/ASN.2009111156; 2010).

With hundreds of poorly regulated clinics that offer unproven stem-cell therapies now running, notably in China and Thailand, the episode is a warning to patients who may be considering such treatment.

The patient had lupus nephritis, in which the immune system attacks the kidneys. In 2006, she underwent a procedure at a private clinic in which her own haematopoietic stem cells — which can develop into any type of blood cell — were injected into her kidneys. Details of the clinic and the rationale behind the treatment have not been released.

“She didn’t get any better from stem cells, we can say that.”

Haematopoietic stem cells have been used to treat lupus nephritis with some reports of success. But they are usually injected into the bloodstream, not the kidney,­ in an attempt to 'reset' the immune system.

Six months later, the patient complained of pain and blood in the urine. Imaging studies revealed a four-centimetre mass on her left kidney and smaller masses in the kidney, liver and adrenal gland. Doctors at Chulalongkorn University in Bangkok removed the kidney, believing a malignant tumour to be present. But further analysis showed that it was something else.

"I had never seen anything like it," says Paul Thorner, a pathologist at the Hospital for Sick Children in Toronto, Canada, who has a joint position at Chulalongkorn University (thailand) and was a co-author on the paper. Thorner coined a term — angiomyeloproliferative — to describe the proliferation of blood-vessel and bone-marrow cells the team found.

Patients are undergoing other experimental stem-cell therapies, but there is usually little follow-up to establish safety or efficacy. In one case reported last year, a boy treated with fetal stem cells at a Russian clinic developed tumours in his brain and spinal cord. In the Thai case, no post-mortem was carried out, as far as Thorner is aware, so his team could work only on the removed kidney. It is not known whether the smaller masses were linked to the main mass, or what would have happened had the patient not died from other complications. "She didn't get any better from stem cells, we can say that," says Thorner, who may try to reproduce the masses in animal experiments.

source: naturenews

International Stem Cell Corporation (OTCBB:ISCO), http://www.intlstemcell.com, a California-based biotechnology company focused on therapeutic and research products, congratulates Advanced Cell Technology, Inc. (ACT) on the issuance of its recent patent, U.S. Patent Number 7,736,896, covering a method for producing retinal pigment epithelial cells.

As licensee of the retinal cell technology covered by this ACT patent, ISCO looks forward to building on this discovery, either independently or in collaboration with ACT, with the goal of advancing the search for treatment of such diseases as Macular Degeneration and Retinitis Pigmentosa, leading causes of blindness in adults, both in the US and the World.

In addition to its licensed interest in the ACT patent, ISCO is developing its own proprietary technology for creating and implanting retinal pigment epithelial (RPE) cells that may be usable either in conjunction with its licensed technology from ACT or independently.

'This is just one more example of the remarkable advancement in science toward the treatment of life's more dreaded diseases, and we are proud to be one of the leading pioneers in that effort,' said Kenneth Aldrich, Chairman of ISCO.

ABOUT INTERNATIONAL STEM CELL CORPORATION (ISCO.OB):

International Stem Cell Corporation is a California-based biotechnology company focused on therapeutic and research products. ISCO's core technology, parthenogenesis, results in creation of pluripotent human stem cells (hpSCs) from unfertilized oocytes (eggs). hpSCs avoid ethical issues associated with the use or destruction of viable human embryos. ISCO scientists have created the first parthenogenic, homozygous stem cell line that can be a source of therapeutic cells with minimal immune rejection after transplantation into hundreds of millions of individuals of differing sexes, ages and racial groups. This offers the potential to create the first true stem cell bank, UniStemCell^(TM), while avoiding the ethical issue of using fertilized eggs. ISCO also produces and markets specialized cells and growth media for therapeutic research worldwide through its subsidiary Lifeline Cell Technology. More information is available at ISCO's website, http://www.internationalstemcell.com.

To subscribe to receive ongoing corporate communications please click on the following link: http://www.b2i.us/irpass.asp?BzID=1468&to=ea&s=0.

FORWARD-LOOKING STATEMENTS

Statements pertaining to anticipated technological developments and therapeutic applications, and other opportunities for the company and its subsidiary, along with other statements about the future expectations, beliefs, goals, plans, or prospects expressed by management constitute forward-looking statements. Any statements that are not historical fact (including, but not limited to statements that contain words such as "will," "believes," "plans," "anticipates," "expects," "estimates,") should also be considered to be forward-looking statements. Forward-looking statements involve risks and uncertainties, including, without limitation, risks inherent in the development and/or commercialization of potential products, uncertainty in the results of clinical trials or regulatory approvals, need and ability to obtain future capital, application of capital resources among competing uses, and maintenance of intellectual property rights. Actual results may differ materially from the results anticipated in these forward-looking statements and as such should be evaluated together with the many uncertainties that affect the company's business, particularly those mentioned in the cautionary statements found in the company's Securities and Exchange Commission filings. The company disclaims any intent or obligation to update these forward-looking statements.

Key Words: Stem Cells, Biotechnology, Parthenogenesis

International Stem Cell Corporation
Kenneth C. Aldrich, Chairman
760-940-6383
kaldrich@intlstemcell.com
or
Brian Lundstrom, President
760-640-6383
bl@intlstemcell.com

International Stem Cell Corporation (OTCBB:ISCO), http://www.intlstemcell.com, a California-based biotechnology company focused on therapeutic and research products, congratulates Advanced Cell Technology, Inc. (ACT) on the issuance of its recent patent, U.S. Patent Number 7,736,896, covering a method for producing retinal pigment epithelial cells.

As licensee of the retinal cell technology covered by this ACT patent, ISCO looks forward to building on this discovery, either independently or in collaboration with ACT, with the goal of advancing the search for treatment of such diseases as Macular Degeneration and Retinitis Pigmentosa, leading causes of blindness in adults, both in the US and the World.

In addition to its licensed interest in the ACT patent, ISCO is developing its own proprietary technology for creating and implanting retinal pigment epithelial (RPE) cells that may be usable either in conjunction with its licensed technology from ACT or independently.

'This is just one more example of the remarkable advancement in science toward the treatment of life's more dreaded diseases, and we are proud to be one of the leading pioneers in that effort,' said Kenneth Aldrich, Chairman of ISCO.

ABOUT INTERNATIONAL STEM CELL CORPORATION (ISCO.OB):

International Stem Cell Corporation is a California-based biotechnology company focused on therapeutic and research products. ISCO's core technology, parthenogenesis, results in creation of pluripotent human stem cells (hpSCs) from unfertilized oocytes (eggs). hpSCs avoid ethical issues associated with the use or destruction of viable human embryos. ISCO scientists have created the first parthenogenic, homozygous stem cell line that can be a source of therapeutic cells with minimal immune rejection after transplantation into hundreds of millions of individuals of differing sexes, ages and racial groups. This offers the potential to create the first true stem cell bank, UniStemCell^(TM), while avoiding the ethical issue of using fertilized eggs. ISCO also produces and markets specialized cells and growth media for therapeutic research worldwide through its subsidiary Lifeline Cell Technology. More information is available at ISCO's website, http://www.internationalstemcell.com.

To subscribe to receive ongoing corporate communications please click on the following link: http://www.b2i.us/irpass.asp?BzID=1468&to=ea&s=0.

FORWARD-LOOKING STATEMENTS

Statements pertaining to anticipated technological developments and therapeutic applications, and other opportunities for the company and its subsidiary, along with other statements about the future expectations, beliefs, goals, plans, or prospects expressed by management constitute forward-looking statements. Any statements that are not historical fact (including, but not limited to statements that contain words such as "will," "believes," "plans," "anticipates," "expects," "estimates,") should also be considered to be forward-looking statements. Forward-looking statements involve risks and uncertainties, including, without limitation, risks inherent in the development and/or commercialization of potential products, uncertainty in the results of clinical trials or regulatory approvals, need and ability to obtain future capital, application of capital resources among competing uses, and maintenance of intellectual property rights. Actual results may differ materially from the results anticipated in these forward-looking statements and as such should be evaluated together with the many uncertainties that affect the company's business, particularly those mentioned in the cautionary statements found in the company's Securities and Exchange Commission filings. The company disclaims any intent or obligation to update these forward-looking statements.

Key Words: Stem Cells, Biotechnology, Parthenogenesis

International Stem Cell Corporation
Kenneth C. Aldrich, Chairman
760-940-6383
kaldrich@intlstemcell.com
or
Brian Lundstrom, President
760-640-6383
bl@intlstemcell.com

Stem cells reverse blindness caused by burns

By ALICIA CHANG (AP) – 1 day ago

LOS ANGELES — Dozens of people who were blinded or otherwise suffered severe eye damage when they were splashed with caustic chemicals had their sight restored with transplants of their own stem cells — a stunning success for the burgeoning cell-therapy field, Italian researchers reported Wednesday.

The treatment worked completely in 82 of 107 eyes and partially in 14 others, with benefits lasting up to a decade so far. One man whose eyes were severely damaged more than 60 years ago now has near-normal vision.

"This is a roaring success," said ophthalmologist Dr. Ivan Schwab of the University of California, Davis, who had no role in the study — the longest and largest of its kind.

Stem cell transplants offer hope to the thousands of people worldwide every year who suffer chemical burns on their corneas from heavy-duty cleansers or other substances at work or at home.

The approach would not help people with damage to the optic nerve or macular degeneration, which involves the retina. Nor would it work in people who are completely blind in both eyes, because doctors need at least some healthy tissue that they can transplant.

In the study, published online by the New England Journal of Medicine, researchers took a small number of stem cells from a patient's healthy eye, multiplied them in the lab and placed them into the burned eye, where they were able to grow new corneal tissue to replace what had been damaged. Since the stem cells are from their own bodies, the patients do not need to take anti-rejection drugs.

Adult stem cells have been used for decades to cure blood cancers such as leukemia and diseases like sickle cell anemia. But fixing a problem like damaged eyes is a relatively new use. Researchers have been studying cell therapy for a host of other diseases, including diabetes and heart failure, with limited success.

Adult stem cells, which are found around the body, are different from embryonic stem cells, which come from human embryos and have stirred ethical concerns because removing the cells requires destroying the embryos.

Currently, people with eye burns can get an artificial cornea, a procedure that carries such complications as infection and glaucoma, or they can receive a transplant using stem cells from a cadaver, but that requires taking drugs to prevent rejection.

The Italian study involved 106 patients treated between 1998 and 2007. Most had extensive damage in one eye, and some had such limited vision that they could only sense light, count fingers or perceive hand motions. Many had been blind for years and had had unsuccessful operations to restore their vision.

The cells were taken from the limbus, the rim around the cornea, the clear window that covers the colored part of the eye. In a normal eye, stem cells in the limbus are like factories, churning out new cells to replace dead corneal cells. When an injury kills off the stem cells, scar tissue forms over the cornea, clouding vision and causing blindness.

In the Italian study, the doctors removed scar tissue over the cornea and glued the laboratory-grown stem cells over the injured eye. In cases where both eyes were damaged by burns, cells were taken from an unaffected part of the limbus.

Researchers followed the patients for an average of three years and some as long as a decade. More than three-quarters regained sight after the transplant. An additional 13 percent were considered a partial success. Though their vision improved, they still had some cloudiness in the cornea.

Patients with superficial damage were able to see within one to two months. Those with more extensive injuries took several months longer.

"They were incredibly happy. Some said it was a miracle," said one of the study leaders, Graziella Pellegrini of the University of Modena's Center for Regenerative Medicine in Italy. "It was not a miracle. It was simply a technique."

The study was partly funded by the Italian government.

Researchers in the United States have been testing a different way to use self-supplied stem cells, but that work is preliminary.

One of the successful transplants in the Italian study involved a man who had severe damage in both eyes as a result of a chemical burn in 1948. Doctors grafted stem cells from a small section of his left eye to both eyes. His vision is now close to normal.

In 2008, there were 2,850 work-related chemical burns to the eyes in the United States, according to the Bureau of Labor Statistics.

Schwab of UC Davis said stem cell transplants would not help those blinded by burns in both eyes because doctors need stem cells to do the procedure.

"I don't want to give the false hope that this will answer their prayers," he said.

Dr. Sophie Deng, a cornea expert at the UCLA's Jules Stein Eye Institute, said the biggest advantage was that the Italian doctors were able to expand the number of stem cells in the lab. This technique is less invasive than taking a large tissue sample from the eye and lowers the chance of an eye injury.

"The key is whether you can find a good stem cell population and expand it," she said.

___

Online:

New England Journal: http://www.nejm.org

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