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Biotechnology, BS – Keiser University

August 4th, 2016 9:36 am

The Bachelor of Science in Biotechnology program trains students in many disciplines including genetics, biochemistry and molecular biology and prepares them for entry into health sciences and analytical / research laboratories. Graduates possess the skills to perform laboratory tests using standardized laboratory procedures.

Graduates of the program will have completed the prerequisites necessary to be successful in graduate programs in the sciences. However, a Bachelor of Science in Biotechnology can also be a terminal program for individuals who wish to work in laboratory settings and other occupations.

The following objectives are designed to meet Keiser Universitys mission and its objectives.

To receive a Bachelor of Science in Biotechnology, students must earn 129 credit hours. Program requirements are as follows:

Lower Division General Education Courses( 31.0 credit hours )

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Biotechnology, BS - Keiser University

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USDA Blog Biotechnology

August 4th, 2016 9:36 am

Federal Activities Report on the Bioeconomy page cover

5/4/2016 UPDATE:

Bioeconomy Webinar Information: Thursday, May 5, 2 p.m.4 p.m. Eastern Time Session Link: https://thinktank.inl.gov/login.html?sessionID=59 Session Passkey: 123 Call in: +1 (562) 247-8422 Access Code: 287-084-886

The USDA and other federal agencies recently released the Federal Activities Report on the Bioeconomy (FARB) documenting federal agency activities aimed at helping to develop and support the bioeconomy an emergingpart of the overall U.S. economy. Emphasis is specifically placed on the production and use of biofuels, bioproducts, and biopower. USDA Chief Scientist and Under Secretary for Research, Education, and Economics (REE), Dr. Catherine Woteki, stresses these fuels, power, and products are produced using biomassagricultural residues, grasses, energy crops, forestry trimmings, algae, and other sourcesinstead of fossil fuels.

The report also delves into the Billion Ton Bioeconomy Vision, an effort coordinated through the Biomass Research and Development (R&D) Board. Comprised of industry experts from the Departments of Energy (DOE), Agriculture (USDA), Interior (DOI), Transportation (DOT), Defense (DoD), and the Environmental Protection Agency (EPA), the National Science Foundation (NSF), and the Office of Science and Technology Policy (OSTP), the Board is committed to collaboration among federal agencies on bioeconomy conceptions working to triple the size of todays bioeconomy by 2030to more than a billion tons of biomass. Read more

Tags: bioeconomy, biofuels, Biomass, BioPreferred, Biotechnology, California, Department of Defense, Department of Energy, Department of Interior, Department of Transportation, Dr. Catherine Woteki, Harry Baumes, National Science Foundation, OCE, REE, U.S. Environmental Protection Agency, White House

Economic Growth, Energy

Innovation, biotechnology and big data are changing the way we produce, distribute and even consume food. From using innovative approaches to improve food safety to sharing market data to assist producers in reaching larger markets, big data and new technologies continue to change the face of agriculture. USDA strives to meet these evolving challenges and will be discussing these issues through the lens of agriculture at the 2015 Agricultural Outlook Forum on Feb. 19-20 in Arlington, Virginia.

Big data isnt just massive amounts of numbers and codes for scientists, researchers and marketers. That information, when interpreted and applied, can help people understand and change the world around them. We are discussing how data helps producers of agricultural commodities in adapting their strategies to meet changing consumer demands, marketing practices and technologies. Read more

Organic meat and poultry producers can now use a streamlined process to get approval for labels verifying that their products do not include genetically engineered (GE) ingredients.

USDAs Food Safety and Inspection Service (FSIS) released new procedures for including a non-genetically engineered statement on the label of organic meat and poultry products. This is consistent with organic regulations, which have always prohibited the use of GE in all organic products. Now, with the new process, it will be easier for certified organic entities to add these claims to existing FSIS-approved products, speeding up the label review process. Read more

Tags: AMS, Biotechnology, Farmers, Food Safety, FSIS, genetic engineering, GMO, Meat and Poultry, National Organic Program, NOP, NRCS, organic, Organic 101, organic certification, Poultry, Tom Vilsack

Food Safety

Emmanuel Amoakwah, a Borlaug Fellow from Ghana currently studying at Ohio State University, gives a presentation on climate change during the Borlaug Symposium at the 2013 World Food Prize on Oct. 16. Approximately 40 Borlaug Fellows and their mentors attended the annual event in Des Moines to network, meet members of the Borlaug family and high-level agricultural officials and this years World Food Prize Laureates. (Photo by Jared Henderson, University of Missouri)

Every year the World Food Prize recognizes the achievements of individuals who have advanced human development by improving the quality, quantity or availability of food in the world. Nobel Peace Prize Laureate Dr. Norman E. Borlaug created the prize, which emphasizes the importance of a nutritious and sustainable food supply for all people.

This years event was held from Oct. 16-19 in Des Moines, Iowa, and also included a USDA-sponsored symposium for 40 foreign scientists from 23 countries (and their university mentors) in the Foreign Agricultural Service Borlaug Fellowship Program. Since 2004, the program has provided U.S.-based training and collaborative research opportunity for scientists and policymakers from developing and middle-income countries to promote food security and economic growth. Read more

Tags: Biotechnology, Borlaug Fellowship Program, Climate Change, coffee, Economic Growth, FAS, food insecurity, Food Security, Iowa, Kenya, Norman Borlaug, Science, Tennessee, World Food Prize

Food and Nutrition, Food Security

Sec. Tom Vilsack visited the grain grading laboratory of GIPSA's Board of Appeals and Review (BAR) and the Grading Service Laboratory (GSL) on October 23, 2013. BAR staff explain their grading review process while BAR Chairman Jim Whalen looks on.

The Grain Inspection, Packers and Stockyards Administrations (GIPSA) National Grain Center (NGC) was proud to host Agriculture Secretary Tom Vilsack on Wednesday, October 23. The NGC, located in Kansas City, MO, is home to the Federal Grain Inspection Services (FGIS) Technology and Science Division along with staff from FGIS Quality Assurance and Compliance Division and Field Management Division.

The grain inspectors, scientists and engineers at the NGC provide a broad spectrum of grain inspection services and support within recently renovated state of the art laboratories. During the visit, NGC staff demonstrated how they oversee, develop and approve methods and instruments used for grain inspection that ensure the consistent standard of measuring quality essential to grain marketing. Read more

On Friday, May 17, 2013, in Mexico City, Mexico USDA Secretary Tom Vilsack led U.S. and Mexican agribusiness representatives in a discussion of priority issues affecting North American agriculture. The roundtables participants represented the breadth and diversity of agricultural trade between the United States and Mexico. Representatives from Grupo Bimbo, Gruma, Driscolls, Cargill, and others joined the Secretary, U.S. Ambassador to Mexico Anthony Wayne, and Acting Deputy Under Secretaries Suzanne Heinen and Max Holtzman to share their views on the opportunities and obstacles facing increased agricultural trade between the United States and Mexico. Read more

Tags: Biotechnology, Climate Change, ethanol, Farm Bill, Food Farm and Jobs Bill, Food Safety, immigration, Mexico, Renewable Energy, Suzanne Heinen, Tom Vilsack

Climate Change, Trade

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USDA Blog Biotechnology

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Virginia Integrative Health – Medical Center – 410 Pine St …

August 4th, 2016 9:36 am

Dr. Ginnan diagnoses with unaparalled precision -- like no other healthcare provider I have seen in either alternative or conventional medicine in over 25 years. I suffer from Ehlers-Danlos syndrome, a connective tissue disorder that contributed to a staggering array of severe ligamentous injuries caused by severe motor vehicle accident. The accident also triggered multiple autoimmune diseases and systemic gastrointestinal disorders.

One of my autoimmune diseases, Mast Cell Activation Syndrome (MCAS), is considered rare. Of the few physicians who are even knowledgeable about it, the vast majority is too scared to touch it because it involves recurrent anaphylaxis - swelling of the throat tissues and resultant airway that can be potentially fatal. Dr. Ginnan is one of, if not the only physician who truly more knowledgeable about my condition than both my medical experts and myself after 20 years of extensive medical research and treatment. For me, this translates into a tangible hope for reversal of my autoimmune disorders. Anyone who suffers from chronic, progressive illness knows hope is everything!!!

If that weren't enough, Dr. Ginnan is as compassionate, as he is courageous in his willingness to fully treat someone with MCAS, in addition to a constellation of complex medical issues. Moreover, his team is as dedicated to exceptional patient care as he is -- true exceptions to today's fragmented and dysfunctional health care system. They are compassionate and take time to listen, while striving to give each and everyone of their patient's the best healthcare available. They utilize cutting-edge medical information from both conventional and alternative disciplines and apply appropriate healing modalities.

Virginia Integrative Associates stands out as outstanding star in contrast to what is fast becoming a failed medicine system in this country. It is the way medicine should be!!

Postscript: This is only the second testimonial I have written about any physician, and I am only doing so now because I am truly impressed with the exceptional level of knowledge and commitment to patient care provided at Virginia Integrative Associates.

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Postnatal human dental pulp stem cells (DPSCs) in vitro …

August 4th, 2016 9:36 am

Dentinal repair in the postnatal organism occurs through the activity of specialized cells, odontoblasts, that are thought to be maintained by an as yet undefined precursor population associated with pulp tissue. In this study, we isolated a clonogenic, rapidly proliferative population of cells from adult human dental pulp. These DPSCs were then compared with human bone marrow stromal cells (BMSCs), known precursors of osteoblasts. Although they share a similar immunophenotype in vitro, functional studies showed that DPSCs produced only sporadic, but densely calcified nodules, and did not form adipocytes, whereas BMSCs routinely calcified throughout the adherent cell layer with clusters of lipid-laden adipocytes. When DPSCs were transplanted into immunocompromised mice, they generated a dentin-like structure lined with human odontoblast-like cells that surrounded a pulp-like interstitial tissue. In contrast, BMSCs formed lamellar bone containing osteocytes and surface-lining osteoblasts, surrounding a fibrous vascular tissue with active hematopoiesis and adipocytes. This study isolates postnatal human DPSCs that have the ability to form a dentin/pulp-like complex.

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Ophthalmology – Journal – Elsevier

August 4th, 2016 9:36 am

Ophthalmology, the journal of the American Academy of Ophthalmology, serves society by publishing clinical and basic science research and other relevant manuscripts that relate to the sense of sight. Excellence is pursued through unbiased peer-review, the advancement of innovation and discovery, and the promotion of lifelong learning.

Disclaimer Notice: Statements and opinions expressed in Ophthalmology are those of the author(s) and are not necessarily those of the Academy or publisher, or indicative of Academy views, policy or editorial concurrence. The Editor(s), the Academy, and the Publisher assume no responsibility for any injury and/or damage to persons or property as a matter of product liability, negligence, or otherwise, or from any use or operation of any methods, products, instructions, or ideas contained in the material herein including those in a language other than English. No suggested test or procedure should be carried out unless, in the reader's judgment, its risk...

Ophthalmology, the journal of the American Academy of Ophthalmology, serves society by publishing clinical and basic science research and other relevant manuscripts that relate to the sense of sight. Excellence is pursued through unbiased peer-review, the advancement of innovation and discovery, and the promotion of lifelong learning.

Disclaimer Notice: Statements and opinions expressed in Ophthalmology are those of the author(s) and are not necessarily those of the Academy or publisher, or indicative of Academy views, policy or editorial concurrence. The Editor(s), the Academy, and the Publisher assume no responsibility for any injury and/or damage to persons or property as a matter of product liability, negligence, or otherwise, or from any use or operation of any methods, products, instructions, or ideas contained in the material herein including those in a language other than English. No suggested test or procedure should be carried out unless, in the reader's judgment, its risk is justified and the patient has received information sufficient to give a fully informed consent. Because of the rapid advances in the medical sciences, medical practitioners should obtain independent verification of diagnoses and drug dosages. Discussions, views, and recommendations as to medical procedures, choice of drugs, and drug dosages are the responsibility of the authors. Consumers should consult with their own doctors about their individual health status before making any health decisions. The Academy does not endorse companies, drugs or other products.

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Ophthalmology

August 4th, 2016 9:36 am

UI Health Care is recognized as one of the best programs in the nation for eye care. Our compassionate staff and skilled specialists provide a comprehensive array of vision and eye health services. Services range from routine vision screenings and eye care to the diagnosis and treatment of complex and rare eye conditions. We also offer the convenience of on-site services for ordering contact lenses and prescription eyewear and solutions such as laser vision correction and refractive surgery.

In addition to our main clinics on the University of Iowa Health Care Campus, several of our eye care services are available for patients and families at Iowa River Landing. Located at the Coralville interchange on Interstate 80 (exit 242), UI Hospitals and Clinics - Iowa River Landing offers patients easy access, free parking, and a state-of-the-art building for delivering outstanding patient care. General eye exams are now available from 8 a.m. to noon on Saturdays at UI Health CareIowa River Landing. Enjoy the convenience of free parking and finding all of your follow-up needs under one roof.

Read more about UI Department of Ophthalmology and Visual Sciences Referrals

We provide a broad range of general eye care services from routine eye examinations to expert prescription and fitting of eye wear and contact lenses.

Eye care services are also available at UI Health Care - River Crossing in Riverside.

UI Healthcare eye specialists are ranked among the best in the world at diagnosing and treating eye diseases. Patients from around the world come to the UI Hospitals and Clinics and UI Children's Hospital to be treated by our staff.

University of Iowa clinicians and researchers provide cutting-edge diagnosis and treatments of eye cancers to patients of all ages.

Our oculoplastic surgeons provide a full range of cosmetic and reconstructive eye services.

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Ophthalmology

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Ophthalmology – Stanford University School of Medicine

August 4th, 2016 9:36 am

Jeffrey L. Goldberg, M.D., Ph.D. Professor and Chairman Department of Ophthalmology

Welcome to the Byers Eye Institute in the Department of Ophthalmology, at Stanford University School of Medicine, a top-tier, internationally recognized, multidisciplinary center combining world-class resources with a commitment to providing the highest level of diagnostic and therapeutic care to our patients.

Through an integrated, personalized approach to healthcare delivery, our dedicated team of specialists, surgeons, nurses, and technicians provides the latest therapies in treating eye disorders such as glaucoma, cataracts, uveitis, and retinal, pediatric, and corneal disease. Associated with Stanford Health Care, and the Lucille-Packard Children's Hospital, our faculty and staff has been providing excellence in ocular and vision healthcare to patients across Northern California and from around the world, while our cutting-edge team of researchers carries out some of the most innovative laboratory research and clinical trials anywhere in the world.

The Department of Ophthalmology's educational mission is focused on clinical service, cutting edge research and innovation, and training the next generation of clinicians and scientists in ophthalmology and visual science. Stanford faculty are trained specialists in Retina, Glaucoma, Cornea and Refractive Surgery, Pediatrics, Oculoplastics, and Neuro-ophthalmology, and are prepared to serve your needs.

I am very proud of our exceptional faculty and staff and their accomplishments in our mission areas of patient care, research, education and community outreach. Whether you are a patient, a resident, or a leader in academic or clinical ophthalmology, I invite you to explore our programs, visit our clinics and operating rooms, and receive your eye care from our premier faculty.

We are here for you.

The new Eye Institute at Stanford, which opened to patients in September 2010, was named for its lead donors, Brook and Shawn Byers. The state-of-the-art facility was formally named The Byers Eye Institute at Stanford. More on The Byers Institute at Stanford

PHILLIPS HEALTHSUITE HACKATHON March 2015

Team 6 Analytics and their app: MediDash In this recent developer "Hackathon", Team 6 and its leader, Robert Chang, MD., Stanford assistant professor of ophthalmology, developed the winning application over one weekend. See the video below:

Read more about the Phillips Healthsuite Hackathon

EyeGo Adapters

EyeGo adapters let you perform eye exams using a smartphone

Developed by Stanford Ophthalmology assistant professor Robert Chang, MD., and ophthalmology resident, David Myung, MD., the system consists of two adapters that are added to an existing smartphone camera. MORE >>

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Ophthalmology – Residency Roadmap

August 4th, 2016 9:36 am

Overview of the Specialty

Ophthalmology deals with the structure, function, diagnosis and treatment of the eye and the visual system. It provides patients with total eye care using medical, surgical and rehabilitative services. Ophthalmologists typically work with a broad range of patients and usually over a long period of time.

Training Requirements

The length of training in ophthalmology must be at least three years. All applicants entering an ophthalmology training program must have taken a general postgraduate clinical year of training in a program accredited by the ACGME or the Royal College of Physicians and Surgeons of Canada. There were 116 ophthalmology residency training programs accredited by the ACGME for 2014/15 that offered 465 advanced positions.

Matching Program Information and Match Statistics

Ophthalmology residency training programs participate in the Ophthalmology Matching Program through SF Match (sfmatch.org). Match results are summarized in the U.S. Match Statistics table below. The ophthalmology matching process is one of the "early match" programs: the matching process is generally completed in late-January (about six weeks prior to the NRMP).

Source:SF Match (sfmatch.org)Opthalmology Residency Match Report.*Competitiveness is based upon the percentage of U.S. Seniors who ranked each specialty as their only choice.

WUSM Match Statistics

For a list of WUSM match statistics for the past several years, please visit Search for WUSM Statistics. You will need to log in with your Medpriv account.

Subspecialty/Fellowship Training

Subspecialty/fellowship training following completion of an ophthalmology training program is available in cornea/external disease, glaucoma, neuro-ophthalmology, ophthalmic pathology, oculoplastics, pediatric ophthalmology, vitreoretinal disease, and uveitis/immunology. Detailed information about the scope of these subspecialty training programs, number of positions offered and length of training is available in the GMED. Further information can be obtained from the American Medical Association and the Fellowship and Residency Electronic Interactive Database Access page. American Medical Association (AMA) FREIDA

Career Information

FREIDA physician workforce information for each specialty includes statistical information on the number of positions/programs for residency training, resident work hours, resident work environment and compensation, employment status upon completion of program and work environment for those entering practice in each specialty.

Washington University Resources

Washington University Graduate Medical Education: GME Information about graduate medical education at Washington University

Washington University Department Website: Department Ophthalmology & Visual Sciences

Faculty Contacts: Ophthalmology

Susan M. Culican, MD, PhD

362-5722

Morton E. Smith, MD*

747-5559

John Lind, MD

362-3857

747-6554

Ophthalmology Elective Recommendations

Search for WUMS Alumni

To search by Specialty, Year, and/or State

National Organizations

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Ophthalmology - Residency Roadmap

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Department of Ophthalmology | Icahn School of Medicine

August 4th, 2016 9:36 am

The Department of Ophthalmology at the Icahn School of Medicine at Mount Sinai consists of faculty and staff based at The Mount Sinai Hospital and New York Eye and Ear Infirmary of Mount Sinai, the nations oldest specialty hospital. The long-term goal of the Department is to advance new diagnostic and therapeutic innovations by enhancing, expanding, and integrating collaborative efforts between our leading clinicians and researchers.

The Department has partnered in research with interdisciplinary institutes at Mount Sinai, including The Friedman Brain Instituteand The Icahn Institute and Department of Genetics and Genomics. Our focus areas for translational research are:

In addition, the Department has continued its longstanding commitment to providing the highest quality, compassionate, and patient-centric care, as well as training and educating the next generation of leaders in eye care and research.

The Department of Ophthalmology at the Icahn School of Medicine at Mount Sinai is committed to upholding a tradition nearly two centuries old of training outstanding residents who will advance the field of ophthalmology.

The Departments training programs, at both New York Eye and Ear Infirmary of Mount Sinai and The Mount Sinai Hospital, provide a wide spectrum of clinical activity. This includes extensive experience in hands-on patient care, as well as cataract surgery, glaucoma procedures, strabismus surgery, eyelid and orbital surgery, cornea and refractive surgery, and retina-vitreous surgery by the completion of the respective residencies. In addition, a comprehensive schedule of lectures and conferences provides an excellent didactic program to complement the clinical experience.

Learn more about the Ophthalmology Residency at New York Eye and Ear Infirmary of Mount Sinai

Learn more about the Ophthalmology Residency at The Mount Sinai Hospital

Research faculty in the Department of Ophthalmology at the Icahn School of Medicine at Mount Sinai engage in both NIH-funded and foundation-funded research, as well as industry-funded clinical trials. The research encompasses bench eye and vision science research, with concentrations in cornea, anterior segment physiology, retina, stem cells, and glaucoma, and all facets of clinical research. Department investigators play national leadership roles in NIH-funded multi-center clinical trials and epidemiologic studies in the fields of Cornea and Uveitis. Department members also participate in NIH-funded clinical trials in glaucoma, retina, and pediatric ophthalmology.

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Mid Michigan Wellness Center | Bay City, MI Acupuncture …

August 4th, 2016 9:36 am

Acupuncture in Bay City, MI | Julie Botimer

Here at Mid Michigan Wellness Center we specialize in practicing Traditional Chinese Medicine which typically focuses on Acupuncture and Herbs plus massage and body work to relieve symptoms and promote healing. Julie graduated from the Pacific College of Oriental Medicine in Chicago, Illinois with a Master of Science degree in Traditional Oriental Medicine. This nationally accredited program includes over 2500 classroom hours and over 900 clinical hours in western and eastern diagnosis, acupuncture, herbal medicine and therapeutic massage. Julie is board certified to practice acupuncture by the National Certification Commission for Acupuncture and Oriental Medicine (NCCAOM) and is a member of MAAOM, AAAOM, and AAC

Acupuncture and Herbs have been used for nearly four thousand years to treat almost every ailment that afflicts mankind.

Dont forget about physical exercise

Believe it or not, when youre exercising your body, youre exercising your mind as well. Aerobic exercise gets your blood pumping, which increases the oxygen going to your brain and lowers your risk of disorders such as diabetes and cardiovascular disease that can lead to memory loss. If you can, start with some exercise in Continue reading

There are more than 80 chronic illnesses that are immune related including rheumatoid arthritis, type 1 diabetes, thyroid disease, inflammatory bowel disease, lupus, and celiac disease.

Autoimmune diseases result when the immune system mistakenly attacks its own bodys tissues. Immune deficiency is more common than most people think; around 20 percent of the population suffers from some form of an Continue reading

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Wisconsin Diabetes Prevention and Control | Wisconsin …

August 4th, 2016 9:36 am

Take the Diabetes Risk Test

Diabetes is a costly, complex, and devastating chronic illness that poses a major public health problem. Diabetes affects over 475,000 adults and 4,500 children and adolescents in Wisconsin. It is the seventh leading cause of death in Wisconsin, costing an estimated annual $6.15 billion in health care costs and lost productivity. Each year, more than 1,100 Wisconsin residents die from diabetes and many more suffer disabling complications, such as heart disease, kidney disease, blindness, and amputations. This burden is higher among minority populations. Much of the health and economic burden of diabetes can be averted through known prevention measures.

The Wisconsin Diabetes Prevention and Control Program (DPCP) is dedicated to improving the health of people at risk for or with diabetes. We rely on our strong partnerships in the development, distribution, and implementation of resources. Please use the links to the left as well as those below to access these resources.

Popular Links

Wisconsin Diabetes Surveillance Report 2012 P-43084 (PDF, 8.6 MB)

2011 Burden of Diabetes in Wisconsin P-00284 (PDF, 2.2 MB)

Diabetes Care Guidelines

Students with Diabetes: A Resource Guide for Wisconsin Schools and Families

Diabetes Self-Care Booklet - English, P-43081 (PDF, 1.2 MB), Spanish P-43081s(PDF, 1.2 MB)

Personal Diabetes Care Record English, P-49357 (PDF, 197 KB)

Blood Sugar Log Booklet English, P-00246 (PDF, 1.3 MB)

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Integrative Medicine | Addiction Medicine | Bellevue …

August 4th, 2016 9:36 am

Creekside Center for Integrative Medicine is a group of independent practitioners who represent backgrounds in either conventional or alternative medicine.

Established in 1986 as Moss Bay Center, Creekside Center for Integrative Medicine was one of the first organizations in the Northwest to offer healthcare using both conventional and alternative medicine in an integrative, team-oriented model.

We are located in Bellevue, Washington and actively serve the Seattle, Bellevue, Redmond, Issaquah and surrounding Washington communities.

With its range of expertise and specialties, Creekside offers an integrated medical experience unparalleled in the Puget Sound area.

Dr. Stephen Markus,is the founder and director Creekside Integrative Medicine Center, and brings fifteen years of experience to integrating both conventional and complementary medicine. Read more

Kay Lamos has been practicing acupuncture since 1985, after graduating in the first class of the Northwest Institute of Acupuncture and Oriental Medicine. Kay has a broad spectrum of training including a degree in Occupational Therapy, and a license in massage therapy. This background gives her an understanding of both western and oriental medicine. Read more

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Faculty – Welcome to the Department of Genetics at Rutgers …

August 4th, 2016 9:36 am

The Department of Genetics currently has 30 faculty members spanning all career stages including tenured, tenure-track, and research-track faculty. Our faculty are actively engaged in scholarship (conducting research, writing grants and peer-reviewed publications, and presenting research at national and international scientific meetings), teaching (teaching courses at Rutgers, lecturing in outside courses, hands-on teaching of post-doctoral fellows, graduate students, and undergraduate students within our laboratories, and participating in numerous activities aimed at educating the general public about the importance of our research), and service(serving on a wide variety of national and local committees, boards, participating in publication and grant peer-review groups).

The research interests of the 30 faculty members span such important areas as: DNA repair mechanisms, instability of cancer cells, molecular evolutionary processes (e.g. gene duplication, enhancer evolution) and evolutionary genetics, gene expression, cellular mechanisms of learning and memory, fertilization (gamete recognition, adhesion, signaling and fusion), and loss of heterozygosity (LOH) for tumor suppressor genes. Several laboratories are actively engaged in human genetics research including searching for genes linked with disease such as Schizophrenia, Autism, Tourette Syndrome, and Alzheimers disease. Other laboratories are applying molecular genetics techniques to model organisms, such as Caenorhabditis elegans (nematode worm), Drosophila melanogaster (fly), mouse, and Escherichia coli (bacterium), to study basic biological processes. We also have faculty working in computational and statistical genetics. For a complete faculty listing and their detailed research descriptions, see our list of faculty or faculty research page.

Members of the faculty have received competitive research grants from several institutes within the National Institutes of Health (NIH) as well as from the National Science Foundation (NSF), the State of New Jersey Commission on Science and Technology and the Commission on Cancer Research, and private foundations including the National Alliance for Research on Schizophrenia and Depression, the Simons Foundation, Autism Speaks, and the March of Dimes.

Our faculty are solely responsible for teaching several undergraduate and graduate courses at Rutgers and play a major role in other team-taught courses. These courses include Introduction to Cancer, Genetics, Genetics Lab, Genetic Analysis I and II, Seminar in Genetics, Genomes, Topics in Molecular Genetics, Topics in Human Genetics, Evolutionary Genetics, Cancer, Quantitative Biology and Bioinformatics, Introduction to Research in Genetics, General Microbiology, Pathogenic Microbiology, Genetics of Compulsive Behavior, Behavioral and Neural Genetics, and Bacterial Physiology.

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Faculty - Welcome to the Department of Genetics at Rutgers ...

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Advocate – Nittor Jayaram, M.D. – Endocrinology – Tinley Park …

August 4th, 2016 9:36 am

AARP MedicareComplete Plus HMO - POS

Aetna Managed Choice (MC) POS

Aetna Signature Administrators PPO

Blue Cross Advocate Employee PPO

Blue Cross Medicare Advantage HMO

Blue Cross Medicare Advantage HMO - POS

Blue Cross Medicare Advantage PPO

Blue Precision Bronze HMO

Blue Precision Platinum HMO

Blue Precision Silver HMO

Cigna Health Partners POS

Cigna Health Partners PPO

Cigna Open Access Plus POS

Cigna Open Access Plus State of Illinois POS

Great West Healthcare - Cigna HMO

Great West Healthcare - Cigna Open Access Plus HMO

Great West Healthcare - Cigna PPO

Humana Advocate Centered EPO

Humana Advocate Centered HMO

Humana Advocate Health Care PPO

Humana DuPage Medical Group HMO

Humana DuPage Medical Group HMO - HMO Select

Humana Illinois Platinum HMO

Humana Integrated Care Program of Illinois

Humana Medicaid Managed Care Plan

Humana National POS - Open Access

Land of Lincoln and Healthlink Choice PPO

Land of Lincoln and Healthlink Complete PPO

Land of Lincoln and Healthlink Confidence PPO

Land of Lincoln and Healthlink Elite PPO

Land of Lincoln and Healthlink Freedom PPO

Land of Lincoln and Healthlink Preferred PPO

Land of Lincoln and Healthlink Premier PPO

Land of Lincoln and PHCS Choice PPO

Land of Lincoln and PHCS Complete PPO

Land of Lincoln and PHCS Confidence PPO

Land of Lincoln and PHCS Elite PPO

Land of Lincoln and PHCS Freedom PPO

Land of Lincoln and PHCS Preferred PPO

Land of Lincoln and PHCS Premier PPO

Land of Lincoln Champion Gold, Silver, Bronze PPO

LLH Adventist Silver PPO 3000

LLH Family Health Network Silver PPO 3100

LLH Swedish Covenant Silver PPO

NALC (Natl Assn of Letter Carriers) Health Benefit

UHC / AARP MedicareComplete (Secure Horizons) HMO

UHC / AARP MedicareComplete Focus HMO

United Healthcare Choice HMO

United Healthcare Choice Plus - Definity

United Healthcare Choice Plus POS

United Healthcare Compass HMO

United Healthcare Group Medicare Advantage PPO

United Healthcare Medicare Solutions PPO

United Healthcare MedicareComplete Plan 1 HMO

United Healthcare Navigate HMO

United Healthcare Navigate POS

United Healthcare Options PPO

United Healthcare Select EPO

United Healthcare Select HMO

United Healthcare Select Plus POS

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Advocate - Nittor Jayaram, M.D. - Endocrinology - Tinley Park ...

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Issues Archive – Cell Therapy News

August 4th, 2016 9:36 am

Biogen Announces Collaboration with University of Pennsylvania on Multiple Gene Therapy Programs

Biogen announced a broad collaboration and alliance with the University of Pennsylvania to advance gene therapy and gene editing technologies. The expansive research and translational development collaboration has multiple objectives, but will primarily focus on the development of therapeutic approaches that target the eye, skeletal muscle and the central nervous system. [Biogen] PressRelease

State Stem Cell Agency Awards Stanford Researchers

Albert Wong receives $2.9 million to develop vaccine for glioblastoma; four others awarded $240,000 each to study bladder, heart and eye conditions. [Stanford School of Medicine] PressRelease

MJFF Supports Stem Cell Projects to Explore Therapies and Provide Research Tools

The Michael J. Fox Foundation (MJFF) announced funding for two projects leveraging the promise of engineered stem cells to speed new therapies and deeper understanding of Parkinsons disease. [The Michael J. Fox Foundation] PressRelease

Novel Immunotherapy Trial for Lymphoma Offers Hope to Patients at Sylvester

Researchers are testing a novel cellular immunotherapy approach to treating patients with diffuse large B-cell lymphoma who have failed standard therapy. This investigational anti-CD19 chimeric antigen receptor T cell therapy, known as KTE-C19, is being studied in a Phase II clinical trial for patients with aggressive non-Hodgkins lymphoma. [University of Miami Miller School of Medicine] PressRelease

Asterias Biotherapeutics Announces Positive New Long-Term Follow-Up Results for AST-OPC1

Asterias Biotherapeutics, Inc. announced new positive long-term follow-up results from its Phase I clinical trial assessing the safety of AST-OPC1 (oligodendrocyte progenitor cells) in patients with spinal cord injury. [Asterias Biotherapeutics, Inc.] PressRelease

FDA Grants Roches Cancer Immunotherapy Tecentriq (Atezolizumab) Accelerated Approval for People with a Specific Type of Advanced Bladder Cancereneration CAR Modifications for Enhanced T-Cell Function

Roche announced that the U.S. Food and Drug Administration (FDA) granted accelerated approval to Tecentriq for the treatment of people with locally advanced or metastatic urothelial carcinoma. [F. Hoffmann-La Roche Ltd.] PressRelease

Nano Dimension and Accellta Joined Forces to Successfully BioPrint Stem Cell-Derived Tissues

Nano Dimension Ltd. announced it has successfully lab-tested a proof of concept 3D Bioprinter for stem cells. The trial was conducted in collaboration with Accellta Ltd. [Nano Dimension Ltd. (PR Newswire Association LLC.)] PressRelease

Regen BioPharma, Inc. Announces ucVax: Universal Donor Cancer Cellular Immunotherapy

Regen BioPharma, Inc. and announced initiation of a preclinical development program aimed at creating the first cord blood based cancer immunotherapeutic product leveraging its NR2F6 immunological checkpoint. [Regen BioPharma, Inc. (PR Newswire Association LLC.)] PressRelease

VM BioPharma Announces FDA Fast Track Designation Granted for Investigational Gene Therapy VM202 for Patients with Amyotrophic Lateral Sclerosis (ALS)

VM BioPharma announced that the U.S. Food and Drug Administration (FDA) has granted Fast Track designation for the companys lead investigational drug, VM202, a Phase II novel gene therapy for the potential treatment of Amyotrophic Lateral Sclerosis. [VM BioPharma] PressRelease

Cryoport to Provide Cold Chain Logistics Support for International Stem Cell Corporations Phase I Clinical Trial for the Treatment of Parkinsons Disease

Cryoport, Inc. announced that it will provide global logistics support to International Stem Cell Corporations (ISCO) Phase I clinical trial in Australia for the treatment of moderate to severe Parkinsons disease. ISCO commenced patient enrollment for the study earlier this month. [Cryoport, Inc.] PressRelease

WPI Team Awarded Patent for Reprograming Skin Cells

Cell therapies for a range of serious conditions, including heart attacks, diabetes, and traumatic injuries, will be accelerated by research at Worcester Polytechnic Institute (WPI) that yielded a newly patented method of converting human skin cells into engines of wound healing and tissue regeneration. [Worcester Polytechnic Institute] PressRelease

Caladrius Biosciences Licenses Cell Therapy Technology for Ovarian Cancer and Subleases Irvine Facility to AiVita Biomedical

Caladrius Biosciences, Inc. announces that it has licensed to AiVita Biomedical, Inc. the exclusive global rights to its tumor cell/dendritic cell technology for the treatment of ovarian cancer. [Caladrius Biosciences] PressRelease

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Issues Archive - Cell Therapy News

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NIHSeniorHealth: Taking Medicines – Personalized Medicines

August 4th, 2016 9:36 am

Medicines: One Size Does Not Fit All

Studies have shown that bad reactions to properly prescribed medicines cause a number of hospitalizations each year. Researchers believe that many of these errors show that when it comes to taking medicines, "one size does not fit all."

For example, allergy medicines simply don't work for everyone who takes them. For some people, taking the standard dosage of a prescription pain reliever such as codeine offers no pain relief, and can even cause side effects that are uncomfortable or life-threatening.

As the body ages, fat and muscle content change, affecting how the body absorbs and processes drugs. Many other factors -- exercise habits, diet, and general state of health -- also influence how a person responds to medications.

Another key factor is heredity -- the genes we inherit from our parents and other ancestors. Genes can influence the way people respond to many types of medicines, such as Tylenol#3, which is acetaminophen plus codeine; antidepressants like Prozac, also called fluoxetine; and many blood pressure and asthma medicines.

Your genes determine the shape and function of your proteins. As drugs travel through the body, they interact with dozens of proteins.

Everyones genes are slightly different, so everyones proteins are different. Variations in some proteins can affect the way we respond to medicines. Such proteins include those that help the body absorb, metabolize, or eliminate drugs.

Many scientists around the country are conducting research to understand how genes affect the way people respond to medicines. This type of research is called pharmacogenomics.

Pharmacogenomics research aims to identify all the possible variations in genes that play a role in drug response. To identify which versions of a certain gene a person has, researchers examine DNA from that person. Researchers can obtain this DNA in an easy, painless and risk-free way using a cotton swab to collect mouth cells from inside the persons cheek. The scientists then isolate the DNA from the mouth cells.

Uncovering differences in people's genetic backgrounds will help doctors prescribe the right medicine in the right amount for each person, making medicines more safe and effective for everyone.

Scientists will also better understand the role that genes play in causing or contributing to diseases including cancer, heart disease, diabetes, depression, and many others. Research in pharmacogenomics will help scientists make future medicines as safe and effective as possible.

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NIHSeniorHealth: Taking Medicines - Personalized Medicines

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Herbs for the Immune System – Blessed Maine

August 4th, 2016 9:36 am

This article will introduce you to a number of herbs that can safely be relied upon to strengthen the immune system, protect you from a wide array of disease-causing organisms and assist you in maintaining vibrant and robust health.

Your immune system is an incredibly complex interaction between organs, glands, body systems, surfaces, cells and chemicals. This symphonic concert of processes requires nourishment in order to function optimally.

Many herbs and other substances are used by cultures around the world to nourish and support immunity and protect us from a multitude of disease causing micro-organisms, including influenza, the Herpes simplex virus, or fungal growths such as Candida. I know a few of these protective and immune strengthening herbs on an intimate level, and would like to introduce you to some of them here. We'll cover astragalus, usnea, sage, garlic, honey, shitake and reishi mushrooms, hyssop, and St. Johns wort.

Milk Vetch Astragalus membranaceus Astragalus has been growing in our gardens for over ten years now. It is quite hardy, and withstands even the coldest Maine winter. It grows into a large bush, quite feathery, bright green and very pretty looking, with dainty, fan-like yellow flowers in mid to late summer.

Oftentimes in nature you will find that the gifts of a plant make themselves known to you in the manner in which the plant grows, the conditions it requires, and its degree of hardiness. When a plant thrives no matter what, take a deeper look, and you may find that it will help you to do the same. Astragalus strikes me as such a plant. Rugged, resilient, strong, powerful, long-lived, graceful, and elegant.

Astragalus is a tonic and restorative food and a potent medicine plant. The Chinese have been using this plant to strengthen immunity for centuries. They say it "strengthens the exterior", or protects against illness. Known as Huang-qi, astragalus is written about in the 2,000 year old Shen Nong Ben Cao Jing, and is still considered to be one of the superior tonic roots in traditional Chinese medicine. It's name literally means yellow leader; yellow referring to the inside of the root, and leader to its medicinal potency.

Mildly sweet, and slightly warm, astragalus invigorates vital energy, is restorative, strengthens resistance, restores damaged immunity, promotes tissue regeneration, is cancer inhibiting, antiviral, adaptogenic, protects and strengthens the heart and the liver, is tonic to the lungs and enhances digestion.

Many scientific studies have verified its immune enhancing action. Astragalus is a powerful "non-specific" immune system stimulant. Instead of activating our defense system against a specific disease organism, astragalus nourishes immunity by increasing the numbers and activity of roving white blood cells, the macrophages.

As an immunostimulant, astragalus engages and activates every phase of of our immune system into heightened activity. In one study, the activity of macrophages was significantly enhanced within six hours of treatment with astragalus, and remained so for the next seventy-two hours.

In Chinese medicine astragalus roots are said to tonify the Spleen, Blood, and Chi. They are used as a tonic for the lungs, for those with pulmonary disease, frequent colds, shortness of breath, and palpitations. Astragalus is also prescribed for those who suffer from fatigue, from any source, chronic nephritis, night sweats, uterine prolapse, or prolapse of the rectum.

It's tissue regenerating and anti-inflammatory abilities make astragalus an excellent ally to heal chronic ulcerations and persistent external infections, as well as to heal hard-to-heal sores and wounds, and to drain boils and draw out pus. Astragalus processed in honey is a specific against fatigue, used to boost vital energy, to nourish the blood, and also against incontinence, bloody urine or diarrhea.

In a study conducted by the University of Texas Medical Center, in Houston, researchers compared damaged immune cells from cancer patients to healthy cells. Astragalus extracts were found to completely restore the function of the cancer patients' damaged immune cells, in some cases surpassing the health and activity of the cells from healthy individuals.

The extract of astragalus was also shown to significantly inhibit the growth of tumor cells in mice, especially when combined with lovage Ligustrum lucidum. According to a study reported in Phytotherapy Research, astragalus appears to restore immunocompetence and is potentially beneficial for cancer patients as well as those suffering with AIDS. It increases the number of stem cells present in the bone marrow and lymph tissue and stimulates their differentiation into immune competent cells, which are then released into the tissues, according to one study reported in the Journal of Traditional Chinese Medicine.

Astragalus also stimulates the production of Interferon, increases its effectiveness in treating disease, and was also found to increase the life span of human cells in culture.

Astragalus protects adrenal cortical function while undergoing chemotherapy or radiation, and helps modify the gastrointestinal toxicity in patients recieving these therapies. Chinese doctors use astragalus against chronic hepatitis, and many studies have demonstrated that astragalus protects the liver against liver-toxic drugs and anti-cancer compounds commonly used in chemotherapy, such as stilbenemide. When used as an adjunct to conventional cancer treatments, astragalus appears to increase survival rates, to increase endurance, and to be strongly liver protective.

Astragalus helps lower blood pressure, due to its ability to dilate blood vessels, and protects the heart. Scientists in the Soviet Union have shown that astragalus protects the heart muscle from damage caused by oxygen deprivation and heart attack.

According to reports in the Chinese Medical Journal, doctors at the Shanghai Institute of Cardiovascular Diseases found that astragalus showed significant activity against Coxsackie B virus, which can cause an infection of the heart called Coxsackie B viral myocarditis, and for which there is no effective treatment. In a follow-up study, researchers found that astragalus helped maintain regular heart rhythms, and beating frequency, and that Coxsackie B patients showed far less damage from the viral infection (as much as 85%).

In Chinese medicine, astragalus is often combined with codonopsis. This compound is said to strengthen the heart and increase the vital energy, while invigorating the circulation of blood throughout the body. It is also traditionally combined with ginseng, and used as a tonic against fatigue, chronic tiredness, lack of energy, enthusiasm, or appetite, and to ease "spontaneous perspiration" or hot flashes.

Japanese physicians use astragalus in combination with other herbs in the treatment of cerebral vascular disease. According to a research paper published by Zhang in 1990, adolescent brain dysfunction improved more with a Traditional Chinese Medicine formula containing astragalus in combination with codonopsis and other herbs than with Ritilin.

Integrating astragalus roots into your winter-time diet, as the Asians have been doing for years, turns out to be a very good idea. Scientists have demonstrated that astragalus will not only prevent colds, but cut their duration in half. Astragalus possesses strong antiviral properties, and in one study regenerated the bronchial cells of virus-infected mice.

Astragalus has been safely used throughout Asia for thousands of years. The Chinese typically slice astragalus roots and add them, along with other vegetables, to chicken broth to create a nourishing and tonic soup. Discard the root after cooking, and consume the broth. No toxicity from the use of astragalus has ever been shown in the millennia of its use in China.

The genus Astragalus is the largest group of flowering plants, with over 2,000 different species, most of which are found in the northern temperate regions. Plants in this genus are amazingly diverse, some are nourishing and medicinal, some useful as raw materials, and others, such as the locoweeds, are toxic. Astragalus membranaceus grows in the wild along the edges of woodlands, in thickets, open woods and grasslands. It is native to the Northeastern regions of China, but grows excellently in Maine soils and temperatures, as do most Chinese medicinal plants we've attempted to grow thus far. Astragalus appreciates deep, well drained, somewhat alkaline soil.

Seeds are easily gathered and when planted in the fall require no prior soaking. They will germinate the following spring as soon as conditions are right. The seeds have a hard seed coat, and some people nick the covering with a file, or soak the seed overnight to hasten germination. Give each plant plenty of room, as much as a foot all around, and harvest after the fourth or fifth year of growth. Use whole or sliced, fresh or dried root for tinctures, honey, infusions, syrup, or in soups.

Astragalus Tincture

St. John's wort Hypericum perforatum St. John's wort contains numerous compounds that possess documented biological actions, and are the focus of much study. Those constituents that have generated the most interest thus far, include the naphthodianthrones, hypericin and pseudohypericin, a wide range of flavonoids, including quercetin, quercitrin, amentoflavone and hyperin, and the phloroglucinols, hyperforin and adhyperforin. Also of interest to researchers are the essential oils, and xanthones.

Wise herbalists have always used the whole herb, and researchers agree, that it is an interaction between the many constituents in St. John's wort, rather than any one active ingredient, that is responsible for the wide range of beneficial actions this healing herb offers.

All parts of the herb are used medicinally, with hypericin content concentrated in the buds and flowers, and also present in top and bottom leaves, as well as the stem, though to a lesser degree.

Activity of Constituents:

Amentoflavone is antiinflammatory and antiulcer.

GABA is a sedative.

Hyperforin is an antibacterial agent active against gram-positive bacteria, is wound healing, a potential anticarcinogenic, and a neurotransmitter inhibitor.

Hypericin is strongly antiviral

Proanthocyanidins are antioxidant, antimicrobial, antiviral, and vasorelaxant.

Pseudohypericin is antiviral and

Quercitrin is a MAO inhibitor, as are the Xanthones.

Xanthones are antidepressant, antimicrobial, antiviral, diuretic, and cardiotonic.

St. John's wort is an excellent wound healer. It possesses strong antimicrobial properties, is a significant antifungal and antibacterial agent, and is especially effective against gram-positive bacteria. It inactivates Escherichia coli at dilutions of 1:400 or 1:200, and is also active against Staphloccus aureus.

Two constituents of the herb, hyperforin and adhyperforin possess antibiotic effects stronger than that of sulfonilamide.

Burns heal rapidly with the application of St. John's wort. In one study using St. Johns'wort oil, first, second, and third degree burns healed at least three times as rapidly than those treated with conventional treatments, and scaring was minimal. Orally administered St. John's wort tincture demonstrated a remarkable healing of incisions, excision and dead space wounds, and has also been shown to inhibit keloid formation.

Studies indicate St. John's wort may enhance coronary blood flow as well as hawthorn, due to the activity of the procyanidins. It significantly increases the production of nocturnal melatonin, which means taking it will help you sleep better, and feel better.

St. John's wort has also shown promise in the treatment of chronic tension headaches, and also appears to be liver-protective. It is a proven antidepressant, best used by those who are mildly to moderately depressed. It is also historically used to treat neurological conditions such as anxiety, insomnia, restlessness, irritability, neuralgia, neuroses, migraines, fibrosis, dyspepsia, and sciatica.

St. Johns wort is an ally when dealing with any fungal problem, such as candida (infusion as sitz bath), thrush (infusion as mouth wash), or an infection on the skin or nails(frequent soaks in infusion). Frequent applications of St. Johns wort oil will also help in healing these infections.

Use the oil to rub on to tired, sore, achy, painful, overworked muscles. St. Johns wort oil is legendary for relieving the pain and inflammation of back-ache, stiff neck, sore shoulders, bad knees, tennis elbow, and anything else that hurts.

St. Johns'wort has shown to be of considerable benefit to patients with Acquired Immune Deficiency Syndrome. (AIDS)

In one study, 16 out of 18 patients stabilized or improved during a 40 month period during which they were treated with St. John's wort. Only 2 of the 16 experienced an opportunistic infection during the time they took the herb.

Many studies have proven that St. John's wort inhibits a variety of viruses, including herpes simplex types 1 and 2, and HIV-1 viruses associated with AIDS. Researchers have concluded that both hypericin and pseudohypericin are uncommonly effective antiviral agents.

The antiviral activity of St. John's wort appears to be somewhat photo-dynamic, involving a photoactivation process to become more intensely effective. Internal use of St. John'swort is not recommended if you are currently taking a pharmaceutical antidepressant.

St. John's wort Tincture

St. John's wort Oil

Sage Salvia officinalis The ancients used aromatic sage to bring the virtues of wisdom, strength and clear thinking. Modern day researchers in Great Britain found that sage inhibits the breakdown of acetylcholine, and so helps to preserve the compound used to prevent and treat Alzheimers.

Sage is loaded with antioxidants, so is anti-aging, and also offers lots of calcium, magnesium, the essential oil, thujone, flavonoids and phytosterols. It is sedating and soothing, and has a tonic effect on the nerves.

Sage is a potent broad spectrum antibiotic, and immune stimulant. It possesses antibacterial, and antiseptic properties and is active against Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, Pseudomonas aeruginosa. E. coli, Candida albicans, Klebsiella pneumoniae, and Salmonella spp.

Some native tribes like the Mohican, commonly chewed the leaves of sage as a strengthening tonic, and people all over the world use sage to build strength and enhance vitality.

Expectorant and diaphoretic, sage is especially effective against sore throat and upper respiratory illness, and infections where there is an excess of mucous. Sage dries up secretions. Sage is also traditionally used, and effective against, dysentery. Its astringent tannins make it an ally for healing mouth sores, canker sores, bleeding gums, and gingivitis, when used as a mouth rinse. A study done in Germany showed that drinking sage infusion on an empty stomach, reduced the blood sugar levels in diabetic patients.

In Italy sage is very commonly used as a seasoning herb. One cannot help but notice the vibrant health and strength of the elder people, which I attribute, at least in part, to their copious use of sage in the diet.

Sage Tincture

Antibiotic Spray

Garlic Allium sativum

Garlic is not only antibacterial, but antiviral, antiseptic, antiparasitic, immune-stimulating, antispasmodic, hypotensive, diaphoretic, antiprotozoan, antifungal, anthelmintic, and cholagogue.

You can rely on the regular use of this spice to keep your body toned and functioning optimally. It will help keep that all-important and vital organ, the heart toned, help keep blood pressure down, as well as help lower cholesterol. Repeated studies have shown that garlic has a beneficial effect on the heart and circulatory system. Chop some into your salad, throw it, simmered in olive oil, over noodles and sprinkle with parsley.

Garlic is rich in antibiotic powers and strengthens the immune system. It is active against both gram positive and gram negative bacteria, including Shigella dysenteriae, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Escherichia coli, Streptococcus spp., Salmonella spp., Camphylobacter spp., Proteus mirablis, and Bacillius anthraxis.

Garlic is also active against herpes simplex, influenza B, HIV and many other serious illnesses. Note that it is active against the food-borne pathogens so often found in commercial foods, Shigella, E. coli, and Salmonella. Garlic kills bacteria in the gastrointestinal tract immediately on contact. To treat an active intestinal bacterial infection, consume lots of raw or cooked garlic, or take garlic capsules.

Garlic in the diet has also been shown to have a beneficial effect on those dealing with cancer, stress, and fatigue. Garlic stimulates the isles of langerhans, increases insulin production, and lowers blood sugar levels, thus aids diabetics in the control of this debilitating disease.

Garlic also helps increase the senovial fluids, and so is an ally for those dealing with arthritis. The sulfur in garlic helps break up the crystallization of uric acid in the joints, and so aids in the relief of gout. Garlic stimulates the brain and has a positive effect on brain functioning, helping to keep us alert and energized. Scientists have found that garlics anti-aging properties not only slowed the destruction of brain cells, but also caused new brain neurons to branch out. An old Ukrainian recipe to keep the mind sharp includes one pound of garlic, ground and added to a jar with the juice of 24 lemons. Leave covered for one moon cycle, then take one teaspoon each night.

Honey Honey is, an ancient Islamic saying goes, the food of foods, the drink of drinks, and the remedy of remedies. The ancient Greeks, Romans and Egyptians all kept honeybees, and extolled the virtues of honey. Some call honey a sweet medicine of heaven, others, elixir of long life. I use honey everyday and you probably should too. Heres why:

Honey is a rejuvenating, revitalizing, invigorating, natural antibiotic substance created by those magical insects, bees. Bees have been called messengers of the gods, and were associated with Great Goddess since the most ancient times. Many legends hint that bees, and their special creation, honey, played a very important role in our human development. It is said that the gifts of honey are long life, good health, and reverence for spirit. Honey has an ancient reputation as a life force increasing, immune strengthening, potency promoting, aphrodisiac elixir.

Honey consists of invert sugar (fructose, dextroglucose) and other sugars. It also contains a complex assortment of enzymes, antibiotic and antimicrobial compounds, organic acids, minerals such as iron, copper, phosphorus, sulfur, potassium, manganese, magnesium, sodium, silicon, calcium, iodine, chlorine, zinc, formic acid, and high concentrations of hydrogen peroxide. Honey also contains varying degrees (it depends on what flowers and herbs the bees are taking their nectar from) of vitamin C, the entire B complex, vitamins D, E, and K, pantothenic acid, niacin, and folic acid, amino acids, hormones, alcohols, and essential oils.

Honey can, and should be, thought of as a super food. It is a live food, stores its vitamins and minerals indefinitely, and is very easily digested by the body. Honey is an all around health and vitality enhancing substance. Wildflower honey, the concentrated nectar of wildflowers, the essence of all the combined medicinal qualities of all the diverse and abundant wild herbs, is thought to be the most medicinal.. All natural, unheated honey is antibiotic, antiviral, antifungal, anti-inflammatory, anticarcinogenic, expectorant, antiallergenic, laxative, antianemic, tonic, immune stimulating, and cell regenerating.

Bees gather the nectar from flowers and store it in their stomach while transporting it back to the hive. During their transport, the dew-laden nectars become concentrated by evaporation. The nectars also combine, in some as yet unexplained way, with the bees digestive enzymes, producing entirely unique compounds. Scientists have measured over 75 different compounds in honey, some of them so complex they have yet to be identified. One thing we can identify however, is the fact that when used as a consistent additive to food and drink, honey increases vitality, energy, immunity, libido, and life force.

Honey is proven more effective than any pharmaceutical antibiotic in the treatment of stomach ulceration, gangrene, surgical wound infections, and speedy healing of surgical incisions. Honey is unsurpassed for the protection of skin grafts, corneas, blood vessels, and bones during storage and transport. In fact, honey is such an excellent preservative of living tissue that it was commonly used to keep dead bodies from decomposing while being transported back to their homeland for burial. After his death in a foreign land, Napoleon was sent home in a huge vat of honey.

The fact that fist size ulcers and third degree burns heal beautifully with frequent applications of pure raw honey is clinically proven, and something I can personally attest to. A few years ago, I got a large third degree burn on my heel during a misstep on a motorcycle tailpipe. It was a deep wound and definitely hampered my ability to get around all that summer. I soaked my burned foot morning and night in lavender and rose salts and after each soaking applied a bandage liberally smeared with pure honey directly over the burn. I kept a thick layer of honey over that burn for a couple of months, and tried as much as possible not to walk on it. Today there is barely a trace of that huge burn hole on the heel of my foot. Since that time, honey is my first treatment of choice for any burn, first, second or third degree, any wounds, no matter how deep, skin ulcers, impetigo, and infections. I just keep whatever it is covered with a thick layer of pure honey. And keep eating it by the spoonful, or drinking it in water, or as mead, depending on what you are trying to nourish and heal.

Honey is active against staph Staphylococcus aureus, strep Streptococcus spp., and Helicobacter pylori, responsible for stomach ulcers, and enterococcus. Honey is also one of my top choices for treating any respiratory condition, whether a cold, flu, or respiratory infection. Honey will be your ally against bronchitis, chronic bronchial and asthmatic problems, rhinitis and sinusitis. Those dealing with chronic fatigue, any wasting disease, a depressed immune system, will all feel the benefits of integrating this sweet medicine of the bees into their daily diets.

Syrups made with pure honey

Usnea Usnea spp. Usnea, or old man's beard as it is commonly called, is a common lichen found hanging from trees around the world. It possesses strong antibacterial and antifungal agents and is a potent immune stimulant.

Usnea has been shown to be more effective than penicillin against some bacterial strains. It completely inhibits the growth of staphylococcus aureus, streptococcus spp., and pneumonococcus organisms. Usnea is effective against tuberculosis, triconomas, candida spp., enterococcus, and various fungal strains, and has also been reported active against Salmonella typhimurium and E.coli.

Usnea is actually two plants in one. The inner plant looks like a thin white stretchy thread or rubber band, especially when wet. The outer plant gives usnea its color and grows around the inner plant. The inner part is a potent immune stimulant, the outer part strongly antibacterial.

Among the known constituents of usnea are usnic acid, protolichesterinic acid, and oreinol derivatives. Usnea is traditionally used around the world against skin infections, upper respiratory and lung infections, and vaginal infections.

It can be dusted as a powder, drank as tea or infusion, used as a wash, bath, soak, douche, or spray. Usnea is also effective in tincture form, 30-60 drops, 4 times daily to boost immunity, 6 times daily to treat an active infection. Drink 2-4 cups of infusion for acute illness. Use 10 drops in an ounce of water and use as a nasal spray to treat sinus infections.

Usnea can sometimes be irritating to delicate mucous membranes of the mouth, nose, and throat, so the tincture should always be diluted in water before using. We walk out into the woods to a big old spruce tree beautifully decorated with long strands of this unique and potent lichen which we gather to make our medicine. Usnea easily absorbs heavy toxic metals and can be potentially toxic, so gather in a clean place.

Usnea Tincture

Shitake Mushroom Lentinus edodes Reishi Ganoderma lucidum, Western reishi/artists conk Ganoderma applanatum Immune activating fungi have been used as allies against disease for millennia. Mysterious mushrooms and fungi are classed in a kingdom all their own. They cannot be called plants, as they are much more primitive, nor are they animal. Fungi actually possess some characteristics of both plant and animal.

There are many common medicinal mushrooms with immune enhancing properties, including maitake, the abundant birch polypores, turkey tails, honey mushrooms, and hens of the woods.

The polypores are commonly given to chemotherapy and radiation patients in Japan, and have been shown to increase survival rates. The body receives deep nourishment from medicinal fungi, as the nutrients and medicinal properties of mushrooms penetrates deep into the bone marrow. So much so, that some have referred to using medicinal mushrooms as herbal bone marrow transplants!

We'll take a deeper look at two of the most widely used medicinal mushrooms, shitake and reishi.

Shitake mushrooms have been used in China for thousands of years to mobilize the immune system to fight off disease. An immunostimulant, shitake increases the activity of the human immune system against any invading organism.

Antiviral, antitumor shitake has been effectively used to treat viral infections, parasites, and cancer. One of its most important constituents, lintinan, has been shown to stimulate immune competent cells, stimulate T-cell production, and increase macrophage activity.

In one study of 23 people with low killer cell activity, and associated fever and fatique for over 6 months, all responded well to taking lintinan, despite not having responded to conventional therapies, including antibiotics and antipyretics.

Studies have shown shitake to be active against viral encephalitis. It also possesses potent anti-tumor activities, and has been shown to prevent metastasis of cancer to the lungs.

Shitake mushrooms are usually added to soups and stews, cooked for about two hours, and then allowed to sit for an additional two hours. Remove the mushrooms before consuming the broth.

Called reishi in Japan, and Ling zhe in China, all the Ganodermas are powerfully immune enhancing, and adaptogens with potent anti cancer properties.

Both sweet and bitter, the Ganodermas are powerful free radical scavengers, eliminating these highly reactive chemicals from the blood stream before they can damage the DNA of healthy cells. Ganodermas are strongly cancer protective, and have been shown to actually help break down and dissolve tumors.

Ganodermas are an excellent addition to the diet of any one who is run down, has been suffering from long term stress, and has low immune function. Either of the Ganodermas effectively increases leukocyte production, promotes lymphatic health, promotes phagocytosis, stimulates T-cells, induces the generation of immunoglobulins, and promotes the multiplication of antibodies.

Scientists from the Tokyo Medical and Dental University demonstrated that the ganoderic acid in these fungi could reduce the cholesterol production in the liver by as much as 95%.

The Ganodermas are heart warming, heart opening, promote serenity, and are said to enhance spiritual powers.

Reishi and artists conk are hard and woody, and are often referred to as shelf mushrooms. They grow on the side of either dead or living trees, and are often found on birch and other hardwoods, or hemlock. Sometimes you will find them growing on the fresh stump of a recently cut or fallen tree, and sometimes on an old stump.

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Herbs for the Immune System - Blessed Maine

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Integrative Medicine | Maharishi University of Management

August 4th, 2016 9:36 am

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Integrative medicine isincreasingly becoming mainstream 30% of Americans use integrative medicine, according to the National Center for Complementary and Integrative Medicine.

What is Integrative Medicine?

Integrative medicine, the future of healthcare, is the integration of traditional alternative medicine with conventional modern medicine. This means, the physician pulls from both alternative and modern medicine methods to find the best approach and healing plan for each individual patient. Integrative medicine puts the patient front and center by looking at the whole person (mind, body, spirit, environment, relationships, and more). This personalized health care approach gives both the physician and patient a greater understanding of the root causes of the disease or discomfort.

Learn more about Integrative Medicine in our free download.

What is Alternative Medicine?

Alternative medicine is the umbrella term used to describe alternative healing techniques such as homeopathy, acupuncture, meditation, ayurveda, yoga, special diets, natural products and other non-invasive techniques. Integrative medicine utilizes these techniques when appropriate, while also relying on western medicine. MUM offers different degree programs and online courses on MaharishiAyurVedaas well as integrative medicine.

How Does Integrative Medicine Work?

Lets look at a simplified example. A patient comes in complaining about frequent headaches. The standard approach might be to run some tests, or simply even just give the patient a prescription for pain relievers. On the other hand, an integrative medicine practitioner would spend time getting to know the patient and what might be the deeper root cause of this issue. They might offer diet and lifestyle changes, suggest some herbs or natural products, or recommend yoga or acupuncture, and so on. If the root cause was potentially more serious, the physician would then pick from western medicine techniques, such as surgeries, scans, tests, and medicine.

There Are Many Practices of Integrative Medicine. Some Include:

To keep up with the growing demand for integrative medicine, about one third of US hospitals now offer integrative medicine health care services, and almost half of all US medical schools have centers or departments for integrative medicine.

Interested in studying integrative medicine? MUM has many degree programs to choose from, including: an undergraduate Pre-Integrative Medicine degree for those that are preparing for medical school, an MD in modern medicine with a concurrent MS in Maharishi AyurVeda and Integrative Medicine with our partner medical school (AUIS), as well as on-campus and online versions of our MS in Maharishi AyurVeda and Integrative Medicine.

Annalisa Fredrickson graduated from MUM with a BFA in Graphic Design and a minor in Business. She is a writer, social media and marketing consultant, health coach and yoga teacher. She loves to travel, create recipes and be in nature.

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Integrative Medicine | The George Washington University

August 4th, 2016 9:36 am

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Human nutrition, the gut microbiome, and immune system …

August 4th, 2016 9:36 am

Nature. Author manuscript; available in PMC 2012 Mar 9.

Published in final edited form as:

PMCID: PMC3298082

NIHMSID: NIHMS360404

Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO 63108

*Contributed equally

1Current address: Section of Microbial Pathogenesis and Microbial Diversity Institute, Yale School of Medicine, New Haven CT 06536

Dramatic changes in socioeconomic status, cultural traditions, population growth, and agriculture are affecting diets worldwide. Understanding how our diet and nutritional status influence the composition and dynamic operations of our gut microbial communities, and the innate and adaptive arms of our immune system, represents an area of scientific need, opportunity and challenge. The insights gleaned should help address a number of pressing global health problems.

A number of reviews have appeared recently about efforts to decipher the interactions between the innate and adaptive immune system and the tens of trillions of microbes that live in our gastrointestinal tracts (the gut microbiota). Here we emphasize how the time is right and the need is great to better understand the interrelationships between diet, nutritional status, the immune system and microbial ecology in humans at different stages of life, living in distinct cultural and socioeconomic settings. Why now? The answer lies in a confluence of forces occurring at the present time that will affect the future. First, there is enormous pressure to devise new ways to feed healthy foods to a human population whose size is predicted to expand to 9 billion by 2050. At the same time, the solutions will have to address the challenges of developing sustainable forms of agriculture in the face of constrained land and water resources 1. Second, there is a great need to develop new translational medicine pipelines for more rigorously defining the nutritional value of foods we consume currently and that we envision creating in the future. These pipelines are required to evaluate health claims made about food ingredients. Third, there is increasing evidence that the nutritional value of food is influenced in part by the structure and operations of a consumers gut microbial community, and that food in turn shapes the microbiota and its vast collection of microbial genes (the gut microbiome) (e.g. ref 2). Therefore, to better define the nutritional value of foods and our nutritional status, we need to know more about our microbial differences and their origins, including how our lifestyles influence the assembly of gut microbial communities in children, and about the transmission of these communities within and across generations of a kinship 3. Fourth, we are learning how our gut microbial communities and immune systems co-evolve during our lifespans and how components of the microbiota impact the immune system. At the same time, we are obtaining more information about how our overall metabolic phenotypes (metabotypes) reflect myriad functions encoded in our human genomes and gut microbiomes. These observations raise the question of how gut microbial community metabolism of the foods we consume affects our immune systems. Fifth, the link between infections that occur within and outside the gut, and the development of nutritional deficiencies has been emphasized for many years. Poor nutrition in turn, increases the risk for infection. Nonetheless, there is still a dearth of mechanistic information that explains these observations. Sixth, only five years remain to achieve the UNs eight Millennium Development Goals (http://www.undp.org/mdg/). Two of these goals relate to human nutrition: goal 1 seeks to eradicate extreme poverty and hunger while goal 4 aims to reduce by two thirds the under-five mortality rate. Up to one billion people suffer from undernutrition of varying degrees, including silent or asymptomatic malnutrition (http://www.fao.org/publications/sofi/en/), making this condition an enormous global health problem. Of the ~10 million children under the age of 5 who die each year, undernutrition contributes in some fashion to >50% of deaths 4. Sadly, children who survive periods of severe undernutrition can suffer long-term sequelae including stunting and neurodevelopmental deficits 5. Moreover, the effects of undernutrition can be felt across generations. Undernourished mothers suffer higher rates of morbidity and mortality and are more likely to give birth to low birth weight children who in turn have increased risk for developing type 2 diabetes, hypertension, dyslipidemia, cardiovascular pathology, and obesity as adults 6. One testable hypothesis is that the gut microbiota may contribute to the risk and pathogenesis of undernutrition through effects on nutrient metabolism and on immune function (). Similarly, does the experience of undernutrition in childhood affect the development of metabolic capacities by this microbial organ in ways that result in persistent metabolic dysfunction or inadequate function, thereby contributing to the sequelae of malnutrition? Finally, if we define malnutrition as the inadequate or excessive consumption of dietary ingredients leading to development of disease, then we need to also consider the alarming epidemic of obesity that is sweeping the world and its relationship to the gut microbiome and immune system.

A schematic of envisioned interrelationships between the gut microbiota, the immune system and diet that underlies the development of malnutrition

We believe that the marriage of two approaches, one involving culture-independent (metagenomic) methods for describing the gut microbiota/microbiome and the other involving gnotobiotics (the rearing of animals under germ-free conditions, with or without subsequent exposure at various stages of postnatal life or adulthood to a microbial species or species consortium) represents a potentially powerful way to address a number of questions related to the interrelationships between diet, nutritional status, the assembly and dynamic operations of gut microbial communities, and the nature of the interkingdom communications between the gut microbiota and host (including host-microbial co-metabolism, and the co-evolution of the immune system2,7,8). Without dismissing caveats related to the use of gnotobiotic models (see below), we describe ways that may be useful for joining gnotobiotics and metagenomic methods to compare and contrast the functional properties of various types of gut microbial communities, to explicitly test or generate hypotheses, and to develop new experimental (and computational) approaches that together inform the design, execution, and interpretation of human studies.

Changes in dietary consumption patterns affect many aspects of human biology. A full understanding of the determinants of nutritional status requires that we know what people are eating and how these diets are changing. Unfortunately, accurate information of this type is hard to obtain and when available generally covers a relatively limited time period. As a corollary, searchable databases that effectively integrate information obtained from the surveillance efforts of many international and national organizations (e.g., WHO, the UN Food and Agriculture Organization, the United States Department of Agriculture (USDA) Economic Research Service) are needed to monitor changing patterns of food consumption in different human populations. Analysis of USDA data tracking the availability of over 200 common food items between 1970 and 2000 reveals that diets in the USA have changed both in terms of overall caloric intake and the relative amounts of different food items (http://www.ers.usda.gov/Data/FoodConsumption). Linear regression of total caloric intake over time shows that the average number of kcal consumed per day increased markedly over this 30-year period (R2=0.911, P<1015). This is consistent with estimates from the US National Health and Nutrition Examination Survey (NHANES), which indicate that adult men and women increased their daily calorie intake by 6.9% and 21.7%, respectively, during the same period 9. If total caloric intake is analogous to primary productivity in macro-ecosystems, where primary productivity is used as a proxy for available energy, then increasing the amount of energy input from the diet would be predicted to affect the number of microbial species living in the gut of a single host, as well as the magnitude of the compositional differences that exist between different hosts or even different regions of a single gut (for discussions about the mechanisms underlying productivity-species richness relationships in macro-ecosystems see refs. 10,11). Intriguingly, metagenomic studies of bacterial composition in the fecal microbiota of obese and lean twin pairs living in the USA have shown that obesity is associated with decreased numbers of bacterial species 3. Reductions in diversity could impact community function, resilience to various disturbances, and the host immune system.

During the past 30-plus years, the American diet has also shifted in terms of the relative contributions of different foods to total energy intake. Since 1970, two dietary epochs can be distinguished based on the contribution of grains to overall calories (mean increase in daily carbohydrate intake for men and women during this period, 62.4g and 67.7g, respectively9). Consumption of other food items has also changed: Spearmans rank correlations between food availability and time, followed by adjustments of p-values to reflect false discovery rates, reveal that the representation of 177 of 214 items tracked by the USDA has either increased or decreased significantly in American diets since 1970. For example, Americans now eat less beef and more chicken, and corn-derived sweeteners have increased at the expense of cane and beet sugars. Additionally, methods of food modification and preparation have changed. Comparable data are needed for other countries with distinct cultural traditions, including countries where people are undergoing dramatic transformations in their socioeconomic status and lifestyles.

We know from metagenomic studies of the human gut microbiota and microbiome that (i) early postnatal environmental exposures play a very important role in determining the overall phylogenetic structure of an adult human gut microbiota, (ii) assembly of the microbiota towards an adult configuration occurs during the first three years of life 12, and (iii) features of the organismal and gene content of gut communities are shared among family members and transmitted across generations of a kinship 3. We also know that dietary habits influence the structure of the human genome. For example, populations that consume diets high in starch have a higher number of copies of the salivary amylase gene (AMY1) than those consuming low-starch diets 13. We know that these habits also affect the gut microbiome. A wonderful illustration of the latter point is the acquisition of a -porphyranase gene that degrades seaweed-associated glycans from marine microbes associated with non-sterile food consumed by Japanese populations. Zobellia galactanivorans is a marine Bacteroidetes that is able to process porphyran derived from marine red algae belonging to the genus Porphyra. Homologs of porphyranases from Z. galactanivorans are present in the human gut bacterium Bacteroides plebeius and prominently represented in the gut microbiomes of Japanese but not North Americans, leading to the suggestion that porphyranases from Z. galactanivorans or another related bacterium were acquired, perhaps through horizontal gene transfer, by a resident member of the microbiota of Japanese consumers of non-sterile food, and that this organism and gene was subsequently transmitted to others in Japanese society14. Together, these observations lead to the notion that systematic changes in overall dietary consumption patterns across a population might lead to changes in the microbiota/microbiome with consequences for host nutritional status and immune responses.

We also know from work in gnotobiotic mice that have received human fecal microbial community transplants that the relative abundances of different bacterial species and genes in the gut microbiota are highly sensitive to the proportions of different foods in the diet 2. Gnotobiotic mice harboring defined collections of sequenced human gut symbionts or transplanted human fecal microbial communities could provide an approach for modeling the effects of different dietary epochs on the gut microbiota and on different facets of host biology. If the desired result is an account of the effects of individual food items or nutrients, then feeding the animals a series of defined diets, each with a different element removed or added might be an appropriate strategy if the food ingredients for the epoch are known and available. If the focus is on the effects of overall differences in dietary habits within or between groups of humans, then diets should reflect the overall nutritional characteristics of the different groups without merely being representative of a single individual. Designing such diets requires detailed accounts of the identity and quantity of each food item consumed, ideally for a large number of people, as well as the methods used for food preparation. The American diet presents a rare opportunity for such an approach, as NHANES datasets (http://www.cdc.gov/nchs/tutorials/Dietary/) provide one-day dietary recall data at multiple timepoints dating back to the early 1970s.

The nexus between nutrient metabolism and the immune system occurs at many levels, ranging from endocrine signaling to direct sensing of nutrients by immune cells. Leptin provides a case study of features of these complex interrelationships. Leptin serves to regulate appetite and is a pleiotropic cytokine, maintaining thymic output and cellularity, and promoting the dominance of Th1 cells over Th2 cells 15,16 while inhibiting the proliferation of T regulatory cells (Tregs) 17. Low levels of leptin may account for the decreased cellular immunity associated with periods of nutrient deprivation 16. Leptin also impacts innate immune cells, ranging from promotion of neutrophil activation and migration to activation of monocytes and macrophages 15. Elegant experiments using mice deficient in the leptin receptor in different cellular compartments traced a requirement for leptin signaling in intestinal epithelial cells for preventing severe disease following exposure to Entamoeba histolytica. Comparisons of db/db mice that lack a functional leptin receptor and their wild-type littermates revealed that leptin controls infectivity and prevents severe inflammatory destruction of the intestine, thereby impacting mortality 18. These studies were extended to mice with engineered mutations in the leptin receptor that are found in human populations (T1138S and T985L, both of which disrupt signaling), Each of these mutations rendered mice more susceptible to E. histolytica infection 18. Leptin levels are significantly reduced in the sera of germ-free mice 19. Moreover, genetically obese leptin-deficient ob/ob mice have marked differences in the taxonomic and gene content of their gut microbial communities 20. To our knowledge, the effects of leptin-receptor deficiency on the gut microbiota have not been reported. Nonetheless, leptin receptor deficiency and E. histolytica pathogenesis provide a setting where the intersections between the endocrine and immune systems, enteric infection, and gut microbial ecology can be explored.

The ability to use macronutrients is essential for the generation and maintenance of a protective effector immune response. Following TCR stimulation and co-stimulation through CD28, the metabolic needs of T cells are met by a dramatic increase in uptake and utilization of glucose, amino acids and fatty acids 21,22. A deficiency in glucose uptake negatively impacts numerous facets of T cell function with impairment of both proliferation and cytokine expression. Similarly, deficiencies in amino acids such as tryptophan arginine, glutamine and cysteine reduce immune activation. Furthermore, TCR stimulation in the absence of co-stimulation, which leads to T cell anergy, has been linked to a failure to upregulate metabolic machinery associated with amino acid and iron uptake 21,22.

Short chain fatty acids (SCFAs) provide one of the clearest examples of how nutrient processing by the microbiota and host diet combine to shape immune responses. SCFAs are end-products of microbial fermentation of macronutrients, most notably plant polysaccharides that cannot be digested by humans alone because our genomes do not encode the large repertoire of glycoside hydrolases and polysaccharide lyases needed to cleave the varied glycosidic linkages present in these glycans 23. These missing enzymes (dining utensils) are provided by the microbiome. The luminal concentration of intestinal SCFAs can be modified by the amount of fiber in the diet: this in turn affects the composition of the microbiota 24. In addition to acting as an energy source for the host, SCFAs exert significant effects on host immune responses. Butyrate can modify the cytokine production profile of helper T cells 25 and promote intestinal epithelial barrier integrity 26, which in turn can help limit exposure of the mucosal immune system to luminal microbes and prevent aberrant inflammatory responses. Production of another SCFA, acetate, by the microbiota promotes the resolution of intestinal inflammation via the G protein-coupled receptor, Gpr43 27. A recent study highlighted the important role of acetate production in preventing infection with the enteropathogen, E. coli 0157:H7. This effect was linked to its ability to maintain gut epithelial barrier function 28. Intriguingly, acetylation of lysine residues may be regulated by SCFA 29 and appears to affect proteins involved in a variety of signaling and metabolic processes. The role of this covalent modification in modulating the activity of proteins intimately involved in innate and adaptive immune responses needs to be explored. It is tempting to speculate that covalent or non-covalent linkage of a variety products of microbial metabolism to host proteins produced within the intestine, or at extra-intestinal sites, will be discovered and found to have important regulatory effects. These different protein modifications could represent a series of mechanisms by which microbial community metabotype is imprinted on the host.

If nutrients and derived metabolites reflect the functional activity of the microbiota, sensors of nutrient/metabolite availability can be considered akin to microbe-associated molecular patterns (MAMPs) that convey information regarding microbes to the host. Several families of innate receptors are involved in recognition of MAMPs: they include Toll-like receptors (TLRs), inflammasomes, C-type lectins such as dectin-1, and RNA-sensing RIG-like helicases such as RIG-I and MDA5. The accompanying review by Maloy and Powrie in this issue provides an overview of this area. Here we would like to emphasize that classical innate immune recognition pathways have evolved to assess the nutrient environment. TLR4 can sense the presence of free fatty acids 30 while ATP is in important activator of the inflammasome 31. A variety of other immune cell-associated sensors serve to couple information about the local nutrient/metabolite environment to the co-ordination of local immune responses. Examples include mTOR (mammalian Target Of Rapamycin), a serine/threonine kinase32, PKR (double stranded RNA-activated protein kinase) 33, the aryl hydrocarbon receptor (AhR) 34, and various nuclear hormone receptors such as liver-X-receptor (LXR) and peroxisome-proliferator activated receptors (PPAR-, , ) 35 ( and ). The mTOR pathway represents an example of how energy availability impacts immune responses. mTOR is activated by PI3 kinase and AKT activity and is inhibited by AMP-activated protein kinase (AMPK), which is a sensor of cellular energy resources. Genetic and pharmacologic approaches (the latter using rapamycin) indicate that mTOR-signaling affects both the innate and adaptive arms of the immune system, including maturation and effector activity of dendritic cells (DCs), inhibition of Treg development, promotion of the differentiation of Th1, Th2 and Th17 cells, regulation of CD8+ T cell trafficking, and inhibition of memory T cell formation 32,36. PKR couples the presence of free fatty acids to immune activation and has been implicated in the pathogenesis of obesity in mice fed a high fat diet, including their immunoinflammatory and insulin-resistant phenotypes 33 (see below). AhR is activated by a variety of agonists, including kynurenine, a product of tryptophan metabolism by indolamine-2,3-dioxygenase (IDO) 37,38. AhR modulates the differentiation of DCs 39 as well as promoting Th17 and Treg differentiation and effector activity 40,41. Withdrawal of tryptophan and arginine controls immune responses 42,43. The presence of an intact amino acid starvation (AAS) response in T cells is essential for the immunosuppressive activity of tryptophan depletion by IDO 44. This example illustrates how the ability of T-cells to sense levels of a nutrient (tryptophan) in its local environment, rather than using the nutrient solely as a fuel source, is an important determinant of cell fate. If assessment of local nutrient levels or metabolites is an important feature in the immune decision-making process, and if the products of microbial metabolism represent heretofore unappreciated agonists or antagonists of immune cell receptors, then an important challenge is to devise in vitro and in vivo models, including genetically manipulatable gnotobiotic animals (e.g., mice or zebrafish) to identify the array of metabolites produced by a microbiota (and host) as a function of different defined diets.

Metabolic sensors that help co-ordinate immune responses

The intestinal microbiota has the capacity to synthesize a variety of vitamins involved in myriad aspects of microbial and host metabolism, including cobalamin (vitamin B12), pyridoxal phosphate (active form of vitamin B6), a cofactor in a variety of enzymatic interconversions involved in amino acid metabolism, pantothenic acid (vitamin B5), niacin (vitamin B3), biotin, tetrahydrofolate (generated from dietary forms of folate) and vitamin K. In addition to vitamin B12, gut microbes produce a range of related molecules (corrinoids) with altered lower ligands including methyladenine, p-cresol, and other analogs. Over 80% of non-absorbed dietary vitamin B12 is converted to these alternate corrinoids 45,46. There is preliminary evidence that syntrophic relationships among members of the human microbiota, and the fitness of some taxa, may be based on the ability to generate, utilize, or further transform various corrinoids46,47.

The ability of the gut microbiota to produce folate and cobalamin could affect host DNA methylation patterns, while acetate produced from microbial fermentation of polysaccharides could modify chromatin structure and gene transcription via histone acetylation. Thus, inheritance of a mammalian genotype, intergenerational transmission of a microbiome, together with a complex dynamic where the microbiome is viewed both as an epigenome per se and as a modifier of the host epigenome during the postnatal period when host, host diet and microbial community co-evolve, could together shape human physiological phenotypes that are manifest during childhood or later in life.

Numerous observational studies indicate that deficiencies in vitamins A, D, E and zinc can adversely impact immune function, particularly T-cell responses. Although a significant body of work exists detailing the myriad effects of vitamin A, D and E on host immune responses, to date there is little evidence for a role of the microbiota in the biosynthesis or metabolism of these vitamins. However, stimulation of dendritic cells via TLR2 increases the expression of host genes associated with generation of the immunoactive form of vitamin A (retinoic acid) while enteric infection has been linked to vitamin A deficiency 48,49. Intriguingly, a recent study demonstrated that vitamin A deficiency leads to a complete loss of Th17 cells in the small intestine of specified pathogen-free mice, and an associated significant reduction in the abundance of segmented filamentous bacteria (SFB) 50, a member of the Clostridiaceae that drives intestinal Th17 responses in mice 51,52. Thus, vitamin A has the potential to modulate immune responses through direct interactions with immune cells, or indirectly by modulating the composition of the microbiota.

The microbiota also affects the absorption of key minerals. Perhaps the best characterized micronutrient in terms of its interaction with both the microbiota and immune system is iron. Iron-deficient mice are resistant to the development of experimental autoimmune encephalomyelitis, and have reduced delayed type hypersensitivity responses and lower levels of IgM and IgG. Iron deficiency also impairs innate immune responses, as it is required for the respiratory burst 53. Likewise, iron is an essential micronutrient for bacteria. Given the low solubility of Fe3+, microbes have evolved the capacity to produce a variety of high affinity iron-binding siderophores.. Microbes take up soluble Fe3+ siderophore complexes via a variety of active transporters. Early studies in gnotobiotic animals revealed a link between the gut microbiota and development of iron deficiency. Germ-free but not conventionally-raised rats become anemic when fed a low iron diet. Germ-free rats also exhibit increased loss of iron in their feces compared to their conventionally-raised counterparts 54. The iron balance that exists between host and microbiota is disturbed in a mouse model of Crohns disease where there is dysregulation of TNF- expression: oral (but not parenteral) iron supplementation in these animals causes a shift in gut microbial community composition, as defined by 16S rRNA-based surveys, and exacerbates their ileitis 55.

Metagenomic methods need to be applied to further delineate the role of the microbiota in iron and other forms of micronutrient deficiency. For example, what is the impact of developing iron deficiency on the configuration of the gut microbiota and microbiome, including its content of siderophores? Does iron repletion return the microbiota/microbiome to a normal pre-deficient state, or are there persistent structural and functional perturbations that require continued nutritional supplementation to correct? Do particular configurations of the microbiota/microbiome predispose the host to iron or other types of micronutrient deficiency? How does the iron content of mothers milk during post-natal life impact the assembly and metabolic operations of the microbiota? In principle, these questions can be first addressed in a variety of gnotobiotic mouse models, and also extended to macronutrient-deficient states.

Obesity, metabolic syndrome and diabetes illustrate the role the diet-microbiota-immune axis plays in shaping human systems biology. Although the dramatic increase in obesity worldwide can be linked to an ever-growing trend towards excessive calorie intake, the microbiota has also been implicated in this disorder. Studies of a cohort of twins living in the USA indicate that the bacterial phylogenetic composition of the fecal microbiota and the representation of microbial genes involved in several aspects of nutrient metabolism in the fecal microbiome are different in lean versus obese twin pairs 3. Different groups applying different primers for amplifying bacterial 16S rRNA genes for culture-independent analyses of gut microbial ecology, and studying different human populations consuming different diets have reported differing results concerning the bacterial phylogenetic composition of the microbiota in lean versus obese individuals 56.

Evidence that a link exists between the microbiota and obesity comes from transplant experiments in gnotobiotic mice: gut communities from leptin-deficient ob/ob mice or mice with diet-induced obesity produce a greater increase in adiposity when transferred to germ-free recipients than do communities from wild-type littermates or mice that have been given a healthy calorically less dense diet 20,57. Germ-free mice are resistant to diet-induced obesity. Additional studies have revealed that the gut microbial community regulates expression of genes that affect fatty acid oxidation and fat deposition in adipocytes. For example, production of the secreted lipoprotein lipase inhibitor angipoietin-like protein 4 (Angptl4; also known as fasting-induced adipose factor) is suppressed by the microbiota: studies of germ-free and conventionalized wild-type and Angptl4/ animals established that microbiota-mediated suppression of gut epithelial expression of this secreted LPL inhibitor results in increased LPL activity and fat storage in white adipose tissue 19,58. TLR5-deficient mice harbor a gut microbiota with a configuration distinct from that encountered in littermate controls. Moreover, when their gut microbiota is transplanted to wild-type germ-free recipients, food intake is increased compared to recipients of microbiota transplants from wild-type mice: increased adiposity and hyperglycemia ensue 59. The mechanism underlying the increase in food consumption remains to be defined although the authors of this study speculate that inflammatory signaling may desensitize insulin signaling in ways that lead to hyperphagia.

Obesity in mice and humans is associated with infiltration of adipose tissue by macrophages, CD8+ T cells 60, and CD4+ T cells 61,62 expressing inflammatory cytokines and chemokines such as TNF-, CCL2, IL-6, IFN- and IL-17 60,62,63. In contrast, adipose tissue in lean mice is home to a population of immunosuppressive regulatory T cells (Treg) that serve to prevent inflammation 64. Mice deficient in the chemokine receptor CCR2 and with obesity induced by consumption of a high-fat diet have reduced macrophage infiltration of the adipose tissue and improved glucose tolerance relative to CCR2-sufficient controls 60, highlighting the role played by factors recruiting inflammatory immune cells and their associated pro-inflammatory products in the pathogenesis of metabolic abnormalities associated with obesity. Blockade of TNF- 65 or expanding Tregs using anti-CD3 mAbs 62 serves to prevent the onset of obesity-associated insulin resistance in a mouse model of diet-induced obesity.

Inflammation drives development of insulin resistance through phosphorylation of insulin receptor 1 (IRS1) via TNF- activated JNK, IKK-, protein kinase C (PKC) or mTOR activity 60. Although MyD88 signals promote development of type 1 diabetes in specified pathogen-free NOD (non-obese diabetic) mice, germ-free MyD88 deficient NOD animals are susceptible to this disorder 66. These findings suggest that particular intestinal microbial configurations can promote or prevent inflammatory immune responses that drive metabolic dysfunction.

Mice fed a high fat diet have increased serum LPS 67. Furthermore, genetically obese mice deficient in leptin or its receptor have reduced intestinal barrier function 68. As noted above, SCFA produced by microbial fermentation affect barrier function. Thus, it will be important to assess whether or not obese humans display similar reductions in barrier function: one scenario is that a high fat diet alters the structure of the intestinal microbiota leading to a reduction in intestinal barrier integrity, enhanced translocation of microbes and/or their antigens resulting in increased microbial antigen load at extra-intestinal sites, enhanced immune stimulation, and the development of insulin-resistance. Furthermore, nutrients are known to directly activate inflammatory arms of the immune system 69. The capacity of the intestinal microbiota to shape immune responses outside of intestine is well documented. Studies have highlighted the ability of the microbiota and specifically SFB to support the development of autoimmune arthritis 70 and experimental allergic encephalomyelitis 71, both of which have been linked to excessive Th17 responses.

Unfortunately, we have scant knowledge of the spatial relationships between members of the microbiota as well as their proximity to elements of the gut-associated immune system in healthy individuals, or individuals with mucosal barrier dysfunction. Gnotobiotic mouse models of obesity may help provide important insights about the biogeography of microbial communities along the length and width of the gut, including whether microbial consortia occupy ectopic sites that could impact the development and perpetuation of barrier dysfunction (e.g., in the crypts of Lieberkuhn where multipotential gut stem cells reside as described in the accompanying article by Medema and Vermeulen). Newer methods, such as CLASI-FISH 72, offer a great deal of promise for characterizing the spatial features of microbe-microbe and microbe-host cell interactions in the gut mucosa, especially if they are applied to gnotobiotic models.

Undernutrition can have a variety of clinical manifestations ranging from mild asymptomatic micronutrient deficiencies to severe, life-threatening conditions such as kwashiorkor or marasmus. Estimates are that implementing current best practice interventions, including lengthening the time of breastfeeding, supplementing diets with zinc and vitamins, improving handwashing and other hygiene measures, and optimizing treatment of acute severe malnutrition, could reduce mortality during the first three years of life by only 25%, even if there is near perfect compliance 5. While a variety of environmental and genetic factors have long been postulated to influence the development of moderate to severe forms of malnutrition 73, the underlying mechanisms remain poorly defined. Food availability, while certainly a major factor, is not the only contributor. For example, in Malawi, the concordance for severe malnutrition between twins within the same household and fed similar diets is only 50% (M. Manary, personal communication). This observation raises a number of questions. Do different configurations of the microbiota predispose one co-twin to kwashiorkor or marasmus? What is the impact of nutrient deficiency, in either the mother or her child, on the configuration of the gut microbiota and microbiome in the developing gut? Does nutrient deficiency in the mother impact the assembly of the microbiota via changes in the mothers gut microbiota or in the nutrient and immune content of her breast milk: both the microbiota and milk are transmitted to the infant yet we have much to learn about how the biochemical and immunologic features of breast-milk change and how breast milk and infant microbiota co-evolve during the suckling period when a mother is healthy or when she is malnourished (see below). If malnutrition delays the maturation of the guts microbial metabolic organ or skews it towards a different and persistent configuration that either lacks necessary functions for health or that expresses functions that may increase the risk for disease, including immunoinflammatory disorders, does nutrient repletion return the microbiota/microbiome to a normal pre-deficient state, or are there persistent structural and functional perturbations that require continued nutritional supplementation to correct? Are there microbiome configurations that correlate with vaccine responsiveness 74?

Studies of severe forms of malnutrition indicate that these patients often have many characteristics of environmental enteropathy 75. Environmental enteropathy, also known as tropical sprue or tropical enteropathy, is a poorly characterized chronic inflammatory disease that primarily affects the small intestine. This disorder afflicts individuals who reside for relatively long periods of time in areas with poor sanitation and who have high exposure to fecal-contaminated water and food. As an example, Peace Corps volunteers returning to the USA from such areas would report a history of diarrheal disease and have signs and symptoms of chronic malabsorption and nutritional deficiencies 76. The malabsorption associated with environmental enteropathy is often subtle, manifesting itself clinically only as stunting due to chronic undernutrition 76. The breakdown in intestinal mucosal barrier function in this disorder can lead to increased susceptibility to enteropathogen infections. Recurrent infections predispose to nutritional deficiencies and further compromise of barrier function, leading to a vicious cycle of further susceptibility to infection and worsening nutritional status77.

Efforts to break this cycle have focused on vaccines that could prevent infection. However, there is significant heterogeneity in the responses to vaccination between children living in highly Westernized societies and children living in certain developing countries. Oral rotavirus vaccine elicits responses in >95% of children living in Westernized societies but only 49% in Malawi 78. Lower oral polio vaccine (OPV) efficacy has been reported in populations with greater enteric disease burden 79. Studies in Chilean children have demonstrated a negative correlation between oral cholera vaccine responses and small bowel bacterial overgrowth 80. In addition, patients with celiac disease, which as noted below, shares phenotypic features with environmental enteropathy, can have a blunted response to parenteral hepatitis B vaccination, but only when their disease is active 81.

Traditionally, the most definitive test for environmental enteropathy has been small intestinal biopsy. Biopsies typically show reductions in small intestinal villus height, increased numbers of intraepithelial lymphocytes, and increased infiltration of the underlying lamina propria by T cells with a predominant Th1 phenotype 75. Some of these features are found in patients with celiac disease, where a luminal antigen (gliadin) drives a T-cell response that, in turn, results in epithelial destruction, reduced absorptive surface area, and malabsorption 76. Unlike celiac disease, the antigens that drive the host immune response in environmental enteropathy are unknown, but there may be an association with certain HLA alleles (e.g., Aw-31 82).

The pathologic events that lead to the development of environmental enteropathy are poorly understood, in part because of the absence of a robust set of readily assayed biomarkers that would improve the ability to diagnose, classify and potentially subcategorize individuals that exhibit the broadly defined clinical manifestations which define this disorder. Epidemiologic data showing a strong association of environmental enteropathy in areas with poor sanitation, occasional epidemic spread of the disease and its responsiveness to antibiotic treatment reinforce the long-standing belief that there is an infectious etiology. While cultures of jejunal aspirates from individuals with environmental enteropathy have suggested contamination of the proximal small bowel by aerotolerent Gram-negative bacteria 83, no single pathogen or set of pathogens has been identified in the gut microbiota of the majority of affected individuals. There is a distinct possibility that this enteropathy is not the result of a single pathogen but rather the result of colonization with microbial consortia that are inflammogenic in the context of a susceptible host. In fact, what constitutes a normal immune repertoire in a healthy gut likely varies considerably depending upon environmental exposures and the configuration of a microbiota. Moreover, most metagenomic studies of the microbiota have focused on members of the domain Bacteria that dominate these communities. Additional tools need to be developed so that they can be extended to viral and eukaryotic components. The latter include parasites that compete for nutrients within the intestines of infected individuals. Parasites can interact directly with bacterial members of the microbiota during their life cycle in ways that promote hatching of parasite eggs, and can shape immune function through factors such as excretory-secretory (ES) products which have been shown to modulate cytokine production, basophil degranulation, immune cell recruitment and interference with TLR signaling 84.

It seems reasonable to posit that individuals living in regions with high oral exposures to fecal contaminated water and foods, and/or with a eukaryotic component of their gut community that includes parasites, will have gut associated-immune systems with significantly different structural and functional configurations than those without these exposures. In this sense, including the term environmental together with enteropathy is logical and emphasizes the need to place a hosts immune and gut microbiome phenotypes in the context of their various exposures.

Comparative metagenomic studies could provide important new diagnostic tools in the form of microbial taxa, and microbiome gene functions whose representation in the gut communities of affected individuals versus healthy controls correlates with environmental enteropathy. In addition, they could provide pathophysiological insights about relationships between host diet, enteropathogen representation in the microbiota, and microbiome gene composition and expression (including expressed metabolic functions). A major challenge will be to correlate this data with the results of quantitative phenotyping of the human guts innate and adaptive immune system. This will require new and safe approaches for sampling system components, especially in the gut mucosa. Similarly, as noted above, we have scant knowledge of the spatial relationships between members of the microbiota, as well as their proximity to elements of the gut-associated immune system in healthy individuals or in individuals with mucosal barrier dysfunction.

Breast milk is known to protect newborns from infection, in part because of the copious amount of maternally generated antibodies that it contains. While these antibodies have specificity for components of the microbiota, the microbial targets are not well defined for given maternal- infant dyads, or as a function of time after delivery. In addition to antibodies, breast milk contains other immunoactive compounds including cytokines (e.g., IL-10), growth factors (e.g., EGF) and antimicrobial enzymes such as lysozyme. The impact of maternal nutritional status on the glycan, protein, lipid and cytokine landscape of breast milk needs to be defined further. This analysis should have a temporal axis that explores co-evolution of the immunological/nutrient properties of mothers milk and the postnatal assembly and maturation of the infant gut microbiota and of the innate and adaptive immune system. Important feedback systems may be revealed. Similarly, knowledge of the vaginal and cutaneous microbiota of mothers prior to and following birth, as a function of their nutritional status could be very informative. For example, are there common configurations of microbial communities occupying these body habitats that correlate with the development of environmental enteropathy in mothers and their offspring?

As noted above, studies have demonstrated the ability of intestinal microbial communities to rapidly re-shape themselves in response to changes in diet. These observations raise the question of whether and how malnourished states impact (i) the spatial/functional organization of the microbiota and the niches (professions) of its component members; (ii) the capacity of the community to respond to changes in diet; (iii) the ability of components of the microbiota to adaptively forage on host-derived mucosal substrates, and (iv) the physical and functional interactions that occur between the changing microbial communities and the intestinal epithelial barrier (including its overlying mucus layer). One way of developing the experimental and computational tools and concepts needed to examine these challenging questions in humans is to turn to gnotobiotic mice who have been humanized by transplantation of gut communities from human donors with distinct physiological phenotypes and to feed these mice diets that are representative of those of the microbiota donor.

We have used metagenomic methods to show that gut (fecal) communities can be efficiently transplanted into germ-free mice and the mice then fed diets that resemble those consumed by the human microbiota donors, or diets whose ingredients are deliberately manipulated in various ways 2. Transplanted human gut microbial communities can be transmitted from gnotobiotic mothers to their pups. In principle, by using mice humanized with microbiota from individuals residing in different regions of the world, and giving them diets that are representative of their cultural traditions, proof-of-principle global clinical trials of the nutritional value of foods and their impact on the microbiota and immune system can be performed.

Transplantation of a human fecal microbiota into germ-free mice can be viewed as capturing an individuals microbial community at a moment in time and replicating it in multiple recipient gut ecosystems. The humanized mice can be followed over time under highly controlled conditions where potentially confounding variables can be constrained in ways that are not achievable in human studies. This type of personalized gnotobiotics also provides an opportunity to determine the degree to which human phenotypes can be transmitted via the gut microbiota as a function of diet. Moreover, the documented responses of microbial lineages and genes encoding metabolic pathways in the transplanted, replicated communities may provide mechanistic insights about differences in the adaptations of healthy versus diseased gut microbiomes (and host immune system) to changes in diets, plus new biomarkers of nutritional status and the impact of various therapeutic interventions, including those based on dietary manipulations. Putative microbial biomarkers obtained from studies of these mice can in turn be used to query datasets generated directly from the human donor(s).

Despite the potential power of using humanized mice to study interactions between the host immune and metabolic systems and the intestinal microbiota under highly controlled conditions, this approach has caveats. Recent work on Th17 responses suggests that unlike the mouse microbiota, which contains SFB, a fecal microbiota from a human donor is not sufficient to drive immune-gene expression in the small intestine of ex-germ free mice52. This raises the possibility that humanization may not fully recapitulate the capacity of a mouse microbiota to mature the intestinal immune system in mice. However, earlier studies on the effects of human microbiota on the mouse immune system revealed that the ability of E. coli heat labile enterotoxin (LT) to break oral tolerance to ovalbumin in germ-free mice can be inhibited by transplantation of either a human or mouse microbiota during the neonatal period 85. Further, a single component of a human gut symbiont, the polysaccharide A component of B. fragilis, is able to mature components of the CD4+ T cell response in mice 86. Finally, we have observed a similar increase in the frequency of TCR-+ cells in the mesenteric lymph nodes of gnotobiotic recipients of a human or mouse microbiota, when compared to germ-free controls (P. Ahern, V. Ridaura and J. Gordon, unpublished observations). This suggests that although not all components of the immune system will be matured by a human gut microbiota, the immune system is not likely to remain ignorant of these communities. In addition, any differences detected in direct comparisons of the effects of two different human communities may represent responses relevant to the human immune system.

We have recently shown that the human fecal microbiota consists largely of bacteria that can readily be cultured87. Metagenomic analysis suggests that the majority of predicted functions in a humans microbiome are represented in its cultured members. In gnotobiotic mice, both complete and cultured communities exhibit similar properties and responses to dietary manipulations. By changing the diet of the host, the community of cultured microbes can be shaped so that it becomes enriched for taxa suited to that diet. These culture collections of anaerobes can be clonally arrayed in multi-well formats: this means that personalized, taxonomically defined culture collections can be created from donors representing different human populations and physiologic phenotypes, and where the microbes have co-evolved and co-existed together within a single human beings gut habitat.

Together, these advances yield a translational medicine pipeline for examining the interplay between food and food ingredients, the microbiota, the immune system and health. Goals for such a human translational medicine pipeline are to (i) identify individuals with interesting phenotypes, (ii) assess transmissibility of their phenotypes via human microbiota transplants into gnotobiotic animals, (iii) select candidate disease-modifying taxa (retrieved from clonally-arrayed, taxonomically defined personal bacterial culture collections), (iv) sequence selected taxa and (v) reunite them in various combinations in gnotobiotic mice as defined model gut communities, so that their interactions with one another and their impact on host biology can be further explored, using a variety of methods [e.g., RNA-Seq, mass-spec based proteomics and metabolomics, multi-label FISH (for biogeographical studies of the microbiota), whole genome transposon mutagenesis (to identify fitness factors for microbes under various dietary contexts 46), immune profiling and other measurements of mucosal barrier function]. Knowing the degree to which tractable bacterial taxa are able to influence host physiology, and how dietary components can be used to affect specific organisms in the microbiota in ways that provide benefit to the host may be very useful for discovering new generations of pro- and prebiotics.

With massive prospective national surveys planned and being implemented, such as the NIHs National Childrens Study that will follow a representative sample of 100,000 children from before birth to age 21, the time is right for an initiative to evaluate the interrelationships between our diets, nutritional status, microbiomes and immune systems. Many components could comprise this initiative. We can readily envision several of these.

As noted above, there is a need to create more and improved databases for monitoring changing patterns of food consumption that integrates the surveillance efforts of a number of organizations. This tool and other interdisciplinary approaches could be used to define a set of study populations representative of established and emerging food consumption patterns in distinct cultural and socioeconomic settings. An emphasis could be placed on comparing humans living in Westernized societies versus those living in developing countries undergoing marked transitions in lifestyles/cultural traditions. New, reliable, cost-effective and generalizable methods will be needed for acquiring quantitative data about the diets consumed by individual humans in these study populations, and the resulting data deposited in searchable databases together with defined annotation standards. Moreover, guidelines need to be further developed related to ethical and legal aspects of human subjects research involving observational and interventional nutritional studies of pregnant women and their offspring.

Readily procured human biospecimens could be used together with high throughput, targeted and nontargeted (quantitative) profiling of metabolites in comprehensive time series studies to define the relationship between diet, nutritional status, and microbiome configuration in healthy individuals at various stages of life (e.g., in women before, during and after pregnancy and in their children during the first 5 years after birth). This could be accompanied by studies of malnourished individuals before, during and after well justified, defined nutritional interventions. In addition to these data, genomes (genotypes), epigenomes and microbiomes could be characterized in these study cohorts together with a variety of clinical parameters (e.g., vaccine responses) and environmental parameters (e.g., water sanitation). The resulting datasets would be deposited in annotated searchable databases. A translational medicine pipeline that includes relevant cellular and animal models would help guide the design and interpretation of these human studies.

As noted above, a major challenge is to obtain cellular and molecular biomarkers for quantitative profiling of the innate and adaptive immune system, including biomarkers of mucosa-associated barrier function. Given the small quantities of biomaterials available from some body sites, this initiative should help advance enabling miniaturizing technology for quantitative measurements of cells and biofluids. Non-invasive imaging-based biomarkers are also needed.

Aspirational goals include identifying new host and microbial biomarkers and mediators of nutritional status, the nutritional value of various foods, the functioning of the human adaptive/innate immune system (including mucosal barrier integrity and mucosal immunity), and the dynamic operations of the microbiota. This information would be used for demonstration projects that rigorously define nutritional health and test preventive or therapeutic recommendations for micro- and macronutrient consumption, for example in pregnant women and infants/children, and their impact on the assembly and operations of their immune systems. The microbiome component could also help define a previously uncharacterized axis of human genetic evolution (our microbiome-evolution) reflecting in part our changing dietary habits. It could also produce testable hypotheses about unappreciated aspects of the pathophysiology of Western diseases, and yield new microbiome-based strategies for disease prevention or treatment.

We are grateful to members of our lab, plus our colleagues Clay Semenkovich and Andrey Shaw, for many helpful discussions. Work cited from our laboratory was supported by grants from the NIH (DK30292, DK70977, DK078669), the Crohns and Colitis Foundation of America, and the Bill and Melinda Gates Foundation.

1. Whitacre PT, Fagen AP, Husbands JL, Sharples FE. Implementing the New Biology: Decadal Challenges Linking Food, Energy, and the Environment. National Research Council of The National Academies of Science; Washington, D.C.: 2010.

11. Mittelbach GG, et al. What is the observed relationship between species richness and productivity? Ecology. 2001;82:23812396.

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