StemCell Therapy

Complete Growth Medium

Table 1. If using the Endothelial Cell Growth Kit-BBE (ATCC PCS-100-040), add the indicated volume for each component:

Component

Volume

Final Concentration

Bovine Brain Extract (BBE)

1.0 mL

0.2%

rh EGF

0.5 mL

5 ng/mL

L-glutamine

25.0 mL

10 mM

Heparin sulfate

0.5 mL

0.75 Units/mL

Hydrocortisone hemisuccinate

0.5 mL

1 g/mL

Fetal Bovine Serum

10.0 mL

2%

Ascorbic acid

0.5 mL

50 g/mL

Table 2. If using the Endothelial Cell Growth Kit-VEGF (ATCC PCS-100-041), add the indicated volume for each component:

Component

Volume

Final Concentration

rh VEGF

0.5 mL

5 ng/mL

rh EGF

0.5 mL

5 ng/mL

rh FGF basic

0.5 mL

5 ng/mL

rh IGF-1

0.5 mL

15 ng/mL

L-glutamine

25.0 mL

10 mM

Heparin sulfate

0.5 mL

0.75 Units/mL

Hydrocortisone hemisuccinate

0.5 mL

1 g/mL

Fetal Bovine Serum

10.0 mL

2%

Ascorbic acid

0.5 mL

50 g/mL

Antimicrobials and phenol red are not required for proliferation but may be added if desired. The recommended volume of each optional component to be added to the complete growth media is summarized in Table 3.

Table 3. Addition of Antimicrobials/Antimycotics and Phenol Red (Optional)

Component

Volume

Final Concentration

Gentamicin-Amphotericin B Solution

0.5 mL

Gentamicin: 10 g/mL

Amphotericin B: 0.25 g/mL

Penicillin-Streptomycin-Amphotericin B Solution

0.5 mL

Penicillin: 10 Units/mL

Streptomycin: 10 g/mL

Amphotericin B: 25 ng/mL

Phenol Red

0.5 mL

33 M

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Endothelial Cell Growth Kit-VEGF ATCC PCS-100-041

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