StemCell Therapy

Dental Pulp Stem Cells were primarily derived from the pulp tissues of exfoliated deciduous teeth, primary incisors and permanent third molar teeth. The dental pulp, third molars in particular, have been shown to be a significant stem cell source due to greater numbers of stem cells residing in the pulp. DPSCs from third molars have been shown to express developmentally important transcription factors, such as oct4, sox2, klf4, nanog and c-myc, which is an indicator of their pluri-potency. Flow-cytometry analysis revealed DPSCs were positive for CD73, CD90, CD105 and CD166, but negative for CD34, CD45 and CD133, suggesting that these cells are mesenchymal-like stem cells. Under specific culture conditions, DPSCs differentiated into osteogenic, adipogenic and neurogenic cells, as well as formed tube-like structures in Matrigel assay.

Stem Cells From Human Exfoliated Deciduous Teeth (SHED) are considered to be the most immature of the Dental Stem Cells and show the highest proliferative capacity when grown in culture. Similar to the DPSCs from third molars, SHED are very durable in culture and have been passaged over 80 times with no detectable signs of senescence or telomere shortening. SHED express the embryonic stem (ES) cell markers Oct4, Nanog, stage-specific embryonic antigens (SSEA-3, SSEA-4), and tumor recognition antigens (TRA-1-60 and TRA-1-81). When cultured in neurogenic medium, SHED form sphere-like clusters which adhere to the culture dish or float freely in the culture medium.

Dental Follicle Stem Cells is an ectomesenchymal tissue surrounding the enamel organ and the dental papilla of the developing tooth germ prior to eruption. The tissue contains the progenitor cells that form the periodontium, (cementum, PDL, and alveolar bone). In culture DFSC adhere to plastic, proliferate well and like other mesenchymal stem cells show a typical fibroblast-like morphology. DFSCs express the embryonic marker OCT4 and the neural progenitor markers Notch-1 and nestin.

Go here to read the rest:
Dental Pulp Stem Cells, Human Dental Pulp Stem Cells ...

This entry was posted on October 28, 2015 at 3:44 pm and is filed under Dental Stem Cells. You can follow any responses to this entry through the RSS 2.0 feed. Both comments and pings are currently closed. |