StemCell Therapy

At the 2010 annual ASPS plastic surgery meeting in Toronto, I listened to Dr. Alexandra Cond Greens research presentation which concluded that 1) you can find quantities of stem cells using centrifugation techniques only without the use of collagenase, 2) the pellet at the bottom of the tube contains the highest % of stem cells and 3) stem cell enhanced fat grafting is better than regular fat grafting.

After she presented her paper, Dr. Peter Rubin, University of Pittsburgh Plastic Surgery Chief, (aka Mr. Stem Cell) said: Dr. Cond Green what you are proposing is quite provocative.

We believe that Dr. Rubin was referring to her findings that you can obtain stem cells by simply centrifuging the fat. This would be the golden minimal manipulation that could keep the FDA out of reach because these stem cells would not be classified as a drug! Dr. Cond Green, a plastic surgeon working with Dr. Pitanguys lab in Brazil, is onto something big here and I have invited her to share her results with you. Please see her comments below.

Figure 1

Autologous fat graft is considered nowadays the ideal soft-tissue filler because it is abundant, host compatible, and can be harvested easily and repeatedly. However many variations in long-term results of transplanted adipose tissue have been reported due to absorption and volume loss. Careful measures are employed to harvest, process and inject the fat in order to get the best possible intake. Still there is no consensus on the best method to prepare the fat and many controversies exist among these different steps.

Also, we have yet to know which part of the adipose tissue is more important for the graft take, the adipocytes, the other cells (stem cells, endothelial cells) or both.(Fig 1)

New evidence points to an improvement in vascularity and blood flow when stem cells are more concentrated in the fat to be transplanted. For therapeutic approaches, reproducible protocols of adipose-derived stem cells isolation and a large amount of cells are necessary, in order to respond to the increasing demand. Therefore, to practically understand what happens when adipose tissue is manipulated in the operating room, we studied the most common methods of fat processing employed by most plastic surgeons, in order to see which method yields the highest quantity of stem cells.

Under loco regional anesthesia, we aspirated adipose tissue from the inferior abdomen of healthy adult females undergoing liposuction with a blunt 3 mm cannula attached to a 10 ml Luer-lock syringe, using the superwet technique (at a rate of 1 ml of a solution containing normal saline with 1:1,000,000 of epinephrine per milliliter of aspirated tissue).

The lipoaspirates were separated into three samples that were processed by decantation (under the action of gravity in the syringe), washing with normal saline (in a ratio of 1:1 (vol/vol), 3 times) and centrifugation at 3,000 rpm/ 1,200g for 3 minutes.

Figure 2

Read the original:
Centrifugation of Fat Tissue Yields More Stem Cells than ...

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