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Mice headed for space to test bone-building drug – Medical Xpress

Sunday, June 4th, 2017

June 2, 2017 by Mirabai Vogt-James A bone densitometer will accompany the mice to the space station. It measures the bone density of the animals. Credit: University of California, Los Angeles

What do space travel, rodents and a bone-building protein all have in common? A team of UCLA scientists is bringing these three elements together to test an experimental drug that could one day result in a treatment for osteoporosis, which affects more than 200 million people worldwide.

The drug could also potentially help those with bone damage or loss, a condition that afflicts people with traumatic bone injury, such as injured military service members, as well as astronauts who lose bone density while in space.

Led by Dr. Chia Soo and Dr. Kang Ting, who met and married while working on this project, as well as Dr. Ben Wu, the UCLA research team is scheduled to send 40 rodents to the International Space Station this week. Once there, the rodents will receive injections of the experimental drug, which is based on a bone-building protein called NELL-1. The project is being done in collaboration with NASA and the Center for the Advancement of Science in Space, which manages the U.S. National Laboratory on the space station.

"This is really a pivotal point in the study of NELL-1's effect on bone density," said Soo, principal investigator on the study, the vice chair for research in the UCLA Division of Plastic and Reconstructive Surgery, and a member of the UCLA Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research. "We would not be at this point without many years of funding and support from the National Institutes of Health, the California Institute for Regenerative Medicine and several UCLA departments and centers. We are honored to conduct the next phase of our research in the U.S. National Laboratory."

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The UCLA researchers have been conducting studies on NELL-1 for more than 18 years and were excited when Julie Robinson, NASA's chief scientist for the International Space Station Program, visited UCLA in early 2014 and encouraged them to submit a grant that would fund their NELL-1 research in space. The team received the necessary funding from the Center for the Advancement of Science in Space in September 2014 to move forward with the project.

"The preparations have been very exciting; we've had conference calls with NASA's Ames Research Center every two weeks to go over all the fine details," said Dr. Jin Hee Kwak, an assistant professor of orthodontics in the UCLA School of Dentistry and project manager on the study. "Everything is choreographed down to the tiniest details, such as whether you're going to fill a syringe half way or all the waythat small amount affects the total weight of the rocket."

SpaceX's Dragon spacecraft is currently targeted to blast off from Kennedy Space Center in Florida today. It will be the first time that UCLA scientists send rodents to the International Space Station. After living in microgravity and receiving NELL-1 injections for about four weeks, half of the rodents will return from space and land in the Pacific Ocean off the coast of Baja, California.

This marks the first time that American researchers will bring back live rodents from the International Space Station. After retrieval, the rodents will be returned to UCLA where they will continue to receive the NELL-1 drug for an additional four weeks. The remaining half of the rodents that stay in the space station will also receive an additional four-week dosage of the drug and will return to UCLA later.

"To prepare for the space project and eventual clinical use, we chemically modified NELL-1 to stay active longer," said Wu, who is chair of the division of advanced prosthodontics in the UCLA School of Dentistry and professor in the schools of engineering and medicine. "We also engineered the NELL-1 protein with a special molecule that binds to bone, so the molecule directs NELL-1 to its correct target, similar to how a homing device directs a missile."

Discovered in 1996 by Ting, NELL-1 has a powerful effect on tissue-specific stem cells that create bone-building cells called osteoblasts. When exposed to NELL-1, the stem cells create osteoblasts that are much more effective at building bone. Furthermore, NELL-1 reduces the function of osteoclasts, which are the cells that break down bone.

"Our preclinical studies show that NELL-1's dual effect on both osteoblasts and osteoclasts significantly increases bone density," said Ting, chair of the section of orthodontics and the division of growth and development in the UCLA School of Dentistry.

After the age of 50, humans typically lose about 0.5 percent of their bone mass each year. But in space, bone loss significantly increases due to the lack of gravity. It is commonly known that bone density is improved by physical activity that puts pressure on bone, which helps it stay strong. Without gravity's pressure, astronauts can lose around 1.5 percent of their bone mass each month. Therefore, space is an ideal testing environment for NELL-1's effect on bone density.

Research on NELL-1 is supported by past or current grants from the National Institute of Dental and Craniofacial Research, the National Institute of Arthritis and Musculoskeletal and Skin Diseases, the California Institute for Regenerative Medicine, the UCLA Broad Stem Cell Research Center, the UCLA School of Dentistry, the UCLA Department of Orthopaedic Surgery and the UCLA Orthopaedic Hospital Research Center.

The experimental NELL-1 drug described above is used in preclinical tests only and has not been tested in humans or approved by the Food and Drug Administration as safe and effective for use in humans.

Explore further: Study reveals bone-building protein's impact on bone stem cells

A new study by UCLA researchers shows that administering the protein NELL-1 intravenously stimulates significant bone formation through the regenerative ability of stem cells.

Growing bone on demand sounds like a space-age concepta potentially life changing one. Such a capability could benefit those needing bone for reconstructive surgery due to trauma like combat injuries or those waging a ...

A UCLA research team has found a combination of proteins that could significantly improve clinical bone restoration. The findings may be a big step toward developing effective therapeutic treatments for bone skeletal defects, ...

UCLA stem cell scientists purified a subset of stem cells found in fat tissue and made from them bone that was formed faster and was of higher quality than bone grown using traditional methods, a finding that may one day ...

Bone morphogenetic protein-2 (BMP2) is used clinically to promote bone repair. However, the high BMP2 concentrations required to stimulate bone growth in humans may produce life-threatening adverse effects such as cervical ...

Researchers from the UCLA Department of Medicine, Division of Hematology Oncology and the Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA have published two studies that define how key ...

When it gets cold around you, your body turns up the heat to maintain its normal temperature. The heat is produced by brown adipose tissue, or brown fat, which also plays a role in how the body uses glucose and fat. However, ...

A University of California, Berkeley, study of mice reveals, for the first time, how puberty hormones might impede some aspects of flexible youthful learning.

A detour on the road to regenerative medicine for people with muscular disorders is figuring out how to coax muscle stem cells to fuse together and form functioning skeletal muscle tissues. A study published June 1 by Nature ...

The bacteria in a child's gut appears to be influenced as early as its first year by ethnicity and breastfeeding, according to a new study from McMaster University.

Cholesterol, a naturally occurring compound at the lung surface, has been shown to have a clear effect on the properties of this nanoscale film that covers the inside of our lungs. Cholesterol levels in this system may affect ...

Researchers from Monash University have developed a new drug delivery strategy able to block pain within the nerve cells, in what could be a major development of an immediate and long lasting treatment for pain.

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Never get another filling? Drug helps regrow your teeth | FOX13 – FOX13 Memphis

Tuesday, May 9th, 2017

Updated: May 9, 2017 - 6:06 PM

FOX13 consumer advisor Clark Howard says new research shows there may be a way to fill cavities by re-growing part of the tooth itself.

Fillings are one of the most common and least expensive dental procedures in America.

JohnSiewekeis the Dental Director at Good Samaritan Health Center in Atlanta. He says while common, fillings are also technique sensitive.

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If you do not follow that technique then you aregonnaget leakage between the tooth and the filling and that'sgonnalead to more decay and more break down and potential pain,Siewekeexplained. Depending on how far that is allowed to go could lead to the loss of the tooth.

Now a team of researchers at Kings College in London think theyve found a way to fill cavities, by stimulating a process that already takes place naturally when a tooth is damaged.

Teeth are a mineralized organ. Highly complex, they contain 2 different types of mineral; the enamel, which is the stuff on the outside that you see and underneath they have another mineral called dentine and right in the middle they have a soft tissue which has got blood supply and nerves, Paul Sharpe said.

Sharpe explained that when damage occurs, stem cells in the pulp are activated and begin the process of producing Dentine to repair the tooth, but it only works when the damage is small.

When the damage is big it just can't cope, the process is not robust enough to repair big holes ... i.e., when a dentist has drilled out decay, he said.

Thats where Tideglusib comes in. Sharpe and his team used the drug to stimulate the process of dentine production in mice.

They placed a small amount of the drug on a sponge and inserted it in the tooth of a mouse where a hole was drilled. The result, Sharpe said, was a massive stimulation of the natural process.

So you get dentine formed and it completely fills the tooth, Sharp said. So the hole that's drilled is now filled with the natural material that's been removed that the tooth has created itself.

It took two to four weeks for the dentine to grow back. Sharpe says its a strong natural repair that could prevent more damage down the road.

Failure with the conventional treatment where you are using cement is that the cement can crack, Sharpe explained. You can get fissures and that can lead to a reinfection of the tooth and there you have to go back to the dentist and have a new one put in.

According to the National Association of Dental Plans, 114 million in the U.S. had no dental coverage at the end of 2016, so making sure new treatments are affordable is key.

Thats one of the reasons Sharpe and his team chose Tideglusib.

It had already been used in patients for treatments in neurological disorders such as Alzheimers, he said. So that means this drug has been used systemically into the bloodstream at high concentrations ... and there is safety data ... so all the costs of drug development -- safety trials and everything else -- we don't have those. That's why I think among other things it could be cost-effective treatment.

Its also a relatively simple process, which also helps to keep cost down.

It's very uncomplicated, Sharpe said. Dentistry is massively high volume/low cost treatment -- so anything that's really over-complicated and expensive is never going to make the market.

It will still be about two years before there are human trials, but Sharpe stresses that even if this treatment goes mainstream, the drug is only meant to repair damage.

Sieweke agrees and says prevention is the best way to keep your dental costs down.

I think a lot of times that is getting lost in the real day-to-day treatment world. The focus is, What's broken? How do we fix it? And unfortunately too often also, What's the cost? And the cheapest way to bring down the cost of dental care is to prevent it, Sieweke said.

Clark says hes excited for the future.

You never know with science, but I think this one is the real deal for your mouth and mine, Clark said.

The best way to keep your dental bill down is to brush your teeth. Do you know how to do it correctly? Clark recently learned that he did not.

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Mouse teeth shown to hold insight into future stem cell tissue regeneration – Bel Marra Health

Wednesday, May 3rd, 2017

Home Health News Mouse teeth shown to hold insight into future stem cell tissue regeneration

The use of stem cells throughout the years has been both a decisive topic and one that holds a lot of promise for potential medical therapy. They are essentially undifferentiated biological cells that havent yet been specialized for a specific purpose. The cells of your heart, stomach, and even your brain have all started out as stem cells, and it wasnt until some point during human development that biological processes channeled them to permanently becoming one type of cell. Scientists and researchers around the globe are always in search of the best way to learn about and harvest these valuable cells, and the latest reports suggest the teeth of rodents are an abundant source.

There are considered two main stem cell types in the body: one is from embryonic development when in the womb, and the other are adult stem cells that exist throughout the body. Harvesting embryonic stem cells has been controversial, as it often seen as unethical, but adult stem cellsfound in organs such as the bone marrow, blood vessel, and liver in mammalsis easier to obtain. Stomach linings, for example, require the constant shedding of their cell linings as the acid wears away at them, and having adult stems cells allows for quick replacement of these sloughed off cells.

Weve all seen mice before, and one of their defining characteristics are their front teeth. What most people arent aware of is that their front teeth, or incisors, constantly grow, as they rely on them to be consistently sharp for burrowing and self-defense, and of course, for eating away at your pantry food. As we grow older our teeth start to wear out, and in nature, once you dont have your teeth anymore, you die. As a result, mice and many other animals from elephants to some primates can grow their teeth continuously. Our labs objective is to learn the rules that let mouse incisors grow continuously to help us one day grow teeth in the lab, but also to help us identify general principles that could enable us to understand the processes of tissue renewal much more broadly, said UC San Franciscos Ophir Klein, MD, Ph.D., a professor of orofacial sciences in UCSFs School of Dentistry and of pediatrics in the School of Medicine.

While not all aspects of this process are fully understood just yet, as the exact signals triggering this process have yet to be identified. It, however, marks an advancement of knowledge in the field, and one that bodes well for the future of stem cell therapy. It may prove beneficial for tissue regeneration to treat everything from severe burns to growing entire organs from scratch.

Related:Stem cells from fat may be useful to prevent aging

Related Reading:

Stem cell technique may aid in bone repair

Osteoporosis can be reversed by stem cell therapy, new potential treatment

https://www.ucsf.edu/news/2017/04/406836/mouse-teeth-providing-new-insights-tissue-regeneration http://www.sciencedirect.com/science/article/pii/S1934590917300942 http://www.medicalnewstoday.com/info/stem_cell

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Baby teeth can be used to make medicines! – Dental News Pakistan

Wednesday, May 3rd, 2017

New research is constantly going on and studies in the world of medicine are rapid and profound. In dentistry, new research has come in that show promising results. It has been deduced that baby teeth contain stem cells and when used to make medicines they can be used to treat quite a few degenerative diseases like leukemia etc. This is the reason why many people in developed countries are banking baby teeth so that regenerative medicine can be produced and then used more frequently.

This practice of preserving baby teeth of children or young adults is known as cryopreserving of wisdom teeth and has been around for some 10 years or so. Even though it is as yet not too common, it is becoming more popular because of its many uses. The reason why this practice is becoming widespread is that research shows that stem cells in baby teeth can help to save lives in the future, be it for dental usage or other medicinal causes.

Stem cell treatment is the future. It can potentially be the difference of life and death for any people going through adverse conditions or diseases. But is it really as advantageous as it is hyped u to be? Some scientists and researchers dont believe so. According to Ben Scheven senior lecturer in oral cell biology in the school of dentistry at the University of Birmingham Research is still mostly in the experimental (preclinical) phase. He went on to say, Dental stem cells may provide an advantageous cell therapy for repair and regeneration of tissues someday.

Dr. Pamela Robey of the National Institute of Dental and Craniofacial Research agrees that research is very progressive and promising but, she believes that there is still very little concrete evidence. She points out that there is still a long way to go before major breakthrough can be made.

Colleague of Dr. Robey, Songtao Shi conducted lots of research on wisdom teeth as well as baby teeth. They discovered that when there was a cavity, the tooth produced a substance known as dentin that filled up the hole in order to protect it. Below this is the pulp where lies nerve tissues and blood supply that provide nourishment to the tooth and gums. Shi deduced from his work that it was the molars that made dentin, not the baby teeth. They had different kind of properties. They not only made dentin, but none like structure too.

The process of stem cell extraction from the pulp is very critical and needs to be cautiously done. The people doing the process need to be very careful to make sure that no infection is mixed in. the stem cells are extracted with the aid of a drill. Scientists claim that small amounts of dental pulp can be used for millions of stem cells.

As per the instructions of the president of American Academy of Pediatric Dentistry, Dr. Jade Miller its critical that the nerve tissue in pulp tissue, the nerve supply and blood supply, still remain intact and alive. He also said that, Typically, the best baby teeth to harvest are the upper front six or lower front six incisors and cuspids.

If parents want to harvest their childrens teeth, they need to make their decision early on and enough their pediatrician so that proper measures can be taken in advance.

Even though the end decision is that of the parents and their children, the American Academy of Pediatric Dentistry encourages dentists to follow future evidence-based literature in order to educate parents about the collection, storage, viability, and use of dental stem cells with respect to autologous regenerative therapies.

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Never get another filling? Drug helps regrow your teeth – WSB Atlanta

Wednesday, May 3rd, 2017

by: Clark Howard Updated: Apr 28, 2017 - 7:53 PM

Channel 2 consumer adviser Clark Howard says new research shows there may be a way to fill cavities by re-growing part of the tooth itself.

Fillings are one of the most common and least expensive dental procedures in America.

John Sieweke is the dental director at Good Samaritan Health Center in Atlanta. He says while common, fillings are also technique-sensitive.

If you do not follow that technique, then you are gonna get leakage between the tooth and the filling and that's gonna lead to more decay and more breakdown and potential pain, Sieweke explained. Depending on how far that is allowed to go could lead to the loss of the tooth.

Now a team of researchers at Kings College in London think theyve found a way to fill cavities, by stimulating a process that already takes place naturally when a tooth is damaged.

Teeth are a mineralized organ. Highly complex, they contain two different types of mineral: the enamel, which is the stuff on the outside that you see, and underneath they have another mineral called dentine, and right in the middle they have a soft tissue which has got blood supply and nerves, Paul Sharpe, a member of the King's College team, said.

Sharpe explained that when damage occurs, stem cells in the pulp are activated and begin the process of producing dentine to repair the tooth, but it only works when the damage is small.

When the damage is big, it just can't cope; the process is not robust enough to repair big holes... i.e., when a dentist has drilled out decay, he said.

Thats where Tideglusib comes in. Sharpe and his team used the drug to stimulate the process of dentine production in mice.

They placed a small amount of the drug on a sponge and inserted it in the tooth of a mouse where a hole was drilled. The result, Sharpe said, was a massive stimulation of the natural process.

So you get dentine formed and it completely fills the tooth, Sharp said. So the hole that's drilled is now filled with the natural material that's been removed that the tooth has created itself.

It took two to four weeks for the dentine to grow back. Sharpe says its a strong natural repair that could prevent more damage down the road.

Failure with the conventional treatment where you are using cement is that the cement can crack, Sharpe explained. You can get fissures and that can lead to a reinfection of the tooth and there you have to go back to the dentist and have a new one put in.

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According to the National Association of Dental Plans, 114 million in the U.S. had no dental coverage at the end of 2016, so making sure new treatments are affordable is key.

Thats one of the reasons Sharpe and his team chose Tideglusib.

It had already been used in patients for treatments in neurological disorders such as Alzheimers, he said. So that means this drug has been used systemically into the bloodstream at high concentrations ... and there is safety data ... so all the costs of drug development -- safety trials and everything else -- we don't have those. That's why I think among other things it could be cost-effective treatment.

Its also a relatively simple process, which also helps to keep cost down.

It's very uncomplicated, Sharpe said. Dentistry is massively high volume/low cost treatment -- so anything that's really over-complicated and expensive is never going to make the market.

It will still be about two years before there are human trials, but Sharpe stresses that even if this treatment goes mainstream, the drug is only meant to repair damage.

Sieweke agrees and says prevention is the best way to keep your dental costs down.

I think a lot of times that is getting lost in the real day-to-day treatment world. The focus is, What's broken? How do we fix it? And unfortunately too often also, What's the cost? And the cheapest way to bring down the cost of dental care is to prevent it, Sieweke said.

Clark says hes excited for the future.

You never know with science, but I think this one is the real deal for your mouth and mine, Clark said.

The best way to keep your dental bill down is to brush your teeth. Do you know how to do it correctly? Clark reecntly learned that he did not.

Here's a tutorial from a dentist on how to brush correctly.

2017 Cox Media Group.

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Never get another filling? Drug helps regrow your teeth - WSB Atlanta

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Saving Baby Teeth May Be Beneficial – Opposing Views

Sunday, April 9th, 2017

Opposing Views
Saving Baby Teeth May Be Beneficial
Opposing Views
Cellular medicine is going to be a big part of what we rely on to rebuild broken parts of the body, said Dr. Joe Laning, the chief technology officer at Store-A-Tooth (a lab that stores stem cells from baby teeth), according to Global News ...

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Dental Stem Cells New York | Dental Stem Cell Recovery …

Tuesday, March 21st, 2017

The restorative properties of stem cells:

Stem cells are unique because they drive the natural healing process throughout your life. Stem cells are different from other cells in the body because they regenerate and produce specialized cell types. They heal and restore skin, bones, cartilage, muscles, nerves and other tissues when injured.

As a result, amazing new medical treatments are being developed to treat a range of diseases contemporary medicine currently deems difficult or impossible to treat. Among them are:

While stem cells can be found in most tissues of the body, they are usually buried deep, are few in number and are similar in appearance to surrounding cells. With the discovery of stem cells in teeth, an accessible and available source of stem cells has been identified. The tooth is natures safe for these valuable stem cells, and there is an abundance of these cells in baby teeth, wisdom teeth and permanent teeth. The stem cells contained within teeth are capable of replicating themselves and can be readily recovered at the time of a planned dental procedure. Living stem cells found within extracted teeth were routinely discarded every day, but now, with the knowledge from recent medical research, Dr. Hershkin provides you the opportunity to save these cells for future use in developing medical treatments for your family.

Aside from being the most convenient stem cells to access, dental stem cells have significant medical benefits in the development of new medical therapies. Using ones own stem cells for medical treatment means a much lower risk of rejection by the body and decreases the need for powerful drugs that weaken the immune system, both of which are negative but typical realities that come into play when tissues or cells from a donor are used to treat patients.

Further, the stem cells from teeth have been observed in research studies to be among the most powerful stem cells in the human body. Stem cells from teeth replicate at a faster rate and for a longer period of time than do stem cells harvested from other tissues of the body.

Stem cells in the human body age over time and their regenerative abilities slow down later in life. The earlier in life that your familys stem cells are secured, the more valuable they will be when they are needed most.

Accessible The stem cells contained within teeth are recovered at the time of a planned procedure: Extraction of wisdom teeth, baby teeth or other healthy permanent teeth.

Affordable when compared with other methods of acquiring and preserving life saving stem cells: Peripheral blood, Bone Marrow, Cord blood etc, recovering Stem Cells from teeth is the most affordable and least invasive.

Convenience the recovery of stem cells from teeth can be performed in the doctors office anytime when a healthy tooth is being extracted.

Ease of Use The recovery of stem cells from teeth does not add any additional time on to a planned procedure.

Why should someone recover and cryopreserve their own stem cells from teeth?Healthy dental pulp contains stem cells that are among the most powerful stem cells in the body and replicate at a faster rate and for a longer period of time than other types of stem cells. Stem cells from teeth show great promise for future regenerative medical treatments of neuro-degenerative diseases, heart disease, diabetes, bone diseases and brain and nerve injuries.

Which teeth are candidates for stem cell recovery and cryopreservation?Any extracted tooth with a healthy pulp contains stem cells. Wisdom teeth, baby teeth and other permanent teeth i.e. healthy teeth that are fractured and teeth recommended for extraction for orthodontic purposes are all candidates for stem cell recovery and cryopreservation.

At what age am I no longer eligible to recover and preserve stem cells from teeth?Age does not seem to play a major factor. All extracted healthy teeth contain stem cells. The younger you are then the younger the cells and these may be more beneficial in future regenerative therapies.

Is one tooth enough or should I try to bank as many teeth as I can as the opportunities arise. I banked deciduous teeth, should I bank third molars?Diseases of different severity or tissue defects of different size will undoubtedly require different amounts of stem cells to heal. Conceptually, the more teeth are banked, the greater the potential for sufficient stem cells to treat various diseases.

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Banking on Baby Teeth: Dental Stem Cells and Regenerative …

Tuesday, March 21st, 2017

A month ago, my 6-year-old wiggled her first tooth out, and the tooth fairy dutifully left a glitter-strewn $1 bill and a nice note. In response to my Facebook post announcing this major milestone, my mom pointed out an article about banking baby teeth because get this the living dental pulp inside baby teeth contains stem cells.

Stem cells might ring a bell for women who delivered babies in a hospital or birth center, because most of us were asked if we wanted to store or donate the stem-cell-rich umbilical cord blood. Stem cells are the bodys biological wild cards, with the potential to be transformed into a variety of other cells and used in medical therapies to replace damaged or malfunctioning cells. Think of it as a way to treat an ailment at a cellular level specific to the individual, rather than just treating symptoms.

For that reason, many parents decide to bank their babys umbilical cord upon birth.

Up to 40 percent of qualifying mothers with normal term pregnancies opt to donate cord blood to the public bank, and private donation is even more frequent, says Dr. Rebecca Haley, medical director of Bloodworks Northwest. Last year alone, 250 units of publicly banked cord blood were sent for transplant through the Cord Blood Coordinating Centerand used in treatments for leukemia, lymphoma, rare cancers and metabolic conditions.

But back to the baby teeth. Growing up around my dads dental office, I saw and learned some fascinating things, but stem cells inside teeth? It blew my mind to think that my childs baby tooth could hold the key to a life-saving treatment in her adulthood.

To collect and store dental stem cells, a dentist must extract the baby tooth when it starts to get wiggly and then prep it with materials from a special kit provided by the chosen dental stem cell bank. Currently there are five such banks located in the United States. Once the doctor preps the tooth, its sent overnight to the chosen bank, where, upon confirming the cells viability, theyre cryogenically preserved (i.e., frozen) until needed.

Currently, this relatively new service is only available privately, which means you have to pay a one-time processing fee that varies from $500 to $1,700, plus an annual storage fee of about $100 to $200. To differentiate themselves, some labs tout higher lab certification standards, options to duplicate cells to enlarge the specimen sample or provide an environmentally friendly processing kit to the dentist. Most labs also affiliate with larger ones, in case the business should change hands or something happens at the storage site.

But arent all stem cells the same? Isnt donating your babys cord, if you choose to do so, enough? Not exactly. There are important differences between dental (mesenchymal) and umbilical cord (hematopoietic) stem cells. Dental stem cells can become, among other options, bone or muscle cells to treat issues associated with those areas of the body, much like doctors already use umbilical cord stem cells in blood-based therapies to regenerate blood and bone marrow for cancer patients.

Also with dental stem cells, you have at least 24 chances (thats the number of baby teeth plus wisdom teeth) to gather them over the years your children lose their teeth. These cells can also be duplicated on a massive scale, so even a small viable sample can theoretically yield a large bounty. Conversely, with umbilical cord stem cells, you get just one chance to gather them at birth. The number of cord stem cells you get is all you get, as there is not yet a method for duplicating them. There are, however, public banks where people can donate or receive umbilical cord stem cells.

So why is it that you havent heard of dental stem cell banking? Its still a work in progress, with many treatments and therapies under development. The U.S. Food and Drug Administrationhas yet to approve the widespread use and application of dental stem cell therapies, with only animal studies and limited clinical human trials conducted thus far. Advocates are hopeful that the successes with umbilical cord stem cell therapies will hasten the approval process for dental stem cell therapies within the next decade.

Think of banking dental stem cells as biological insurance, says Arthur E. Greco, CEO of StemSave, a dental stem cell bank in New York City. He and other supporters of dental stem cells believe regenerative therapies are poised to revolutionize medicine.

Young people today are projected to have life spans of 100-plus years, says Greco. Regenerative therapies will play a central role in assuring that those longer life spans will be healthy as stem cell treatments are utilized to combat the normal degradation that occurs as we age.

While this may sound like science fiction, medicine is moving toward, customizing therapies and medications down to the cellular level. There is still much work and research needed, but by the time our kids hit middle age, this type of treatment could be a distinct reality.

This area of study is moving quickly, and significant clinical applications may be available in the future, says American Academy of Pediatric Dentistrynational spokesperson Dr. Amr M. Moursi. Parents should discuss the risks and benefits of dental stem cell banking with their pediatric dentist in order to make a well-informed decision.

While its not a decision to take lightly, Seattle pediatric dentist Dr. Purva Merchantembraces dental stem cell collection. Stem cells are becoming more and more invaluable in retaining genetic information that is specific to that particular individual, she says. This will help in customizing medication for certain genetic conditions.

If youre interested in dental stem cell banking, read up on all of the options and find the one that best fits your needs and budget for the long haul. After all, youre setting up a potential option for your childrens medical well-being that will follow them into adulthood. While some parents may be ready to jump on the dental stem cell bandwagon now, others might want to wait and keep tabs on future medical research partnerships and FDA trials. Either way, I bet youll never look at a loose baby tooth the same way again. I know I wont.

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We May Have Finally Found the Foundations Upon Which Life Evolved – Futurism

Tuesday, March 21st, 2017

Powering Life

Scientists have discovered that a version of the Krebs cycle, the heart of the cellular metabolic network, can take place without the cellular proteins known as enzymes. Since the Krebs cycle does not require cellular proteins to occur, researchers now believe that metabolism may predate life. In fact, spontaneous chemical reactions may have served as the foundation for life on Earth.

Metabolism describes the web of chemical reactions that maintain the living state of cells and organisms. This includes both reactions that synthesize amino acids and lipids that cells need and reactions that break down molecules to generate energy. Cells use lipids and amino acids in membranes and proteins and to create the molecules that are consumed to generate energy.

The intriguing question scientists now face is this: how did this complex cycle evolve at all if it predated life?

Two main theories about the evolution of the Krebs cycle have been proposed. One says that RNA came first, prompting the evolution of the Krebs cycle. However, not only is RNA made from metabolic products, evolutionary principles suggest that the reactions must have predated life; existing in the first life forms immediately, these chemical reactions offered some kind of advantage, a selective pressure which eventually resulted in the evolution of enzymes.

The other theory is that some form of the Krebs cycle existed before life forms did, and was then adopted by living cells. The cycle then evolved inside and with life forms, developing enzymes to become more efficient. This theory was dismissed by many in the past, but these latest findings published in Nature Ecology & Evolutionlend it credence.

The research team exposed Krebs cycle chemicals to chemicals that would have been present in the sediments of early oceans. Eventually they triggered 24 chemical reactions in sequence something very similar to todays Krebs cycle. Thus far, however, they have only shown that this cycle runs in the oxidative direction, a development that would have taken place only once there was molecular oxygen in Earths atmosphere. They have yet to generate the reductive Krebs cycle that is still present in some ancient bacteria.

The researchers point out that there are still key components of life that this work does not explain, said Mark Ralser of the Francis Crick Institute in London, leader of the team who reported the findings.

With the metabolic pathway alone, you have a very good starting point for life, but it is not life, just a chemical-reaction network, Ralser said in an interview with New Scientist. You also need things like membranes to contain the reactions, and the genetic machinery that enables inheritance.

How do you bring these elements together in one environment and in non-extreme conditions, and make them work? Ralser asked. This is still a big challenge.

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6 Weird Things That Happen With Kids’ Teeth – POPSUGAR

Tuesday, March 21st, 2017

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6 Weird Things That Happen With Kids' Teeth
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In a new push for banking stem cells, companies like Tooth Bank are storing dental stem cells, which have the ability to regenerate into various cell types. A dental professional extracts your child's baby tooth, then dental stem cells are harvested ...
Tooth Regeneration Market Global Industry Insights, Trends, Outlook, and Opportunity Analysis, 2016-2024satPRnews (press release)

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Okayama University Research: Bioengineered Tooth Restoration in … – Benzinga

Tuesday, March 21st, 2017

Researchers at Okayama University report in Scientific Reports successful tooth regeneration in a postnatal large-animal model. The approach used involves the autologous transplantation of bioengineered tooth germ into a canine jawbone; the in vivo artificially created tooth has the structure, composition and physiological characteristics of a natural tooth.

Okayama, Japan (PRWEB UK) 19 March 2017

Source: Okayama University (JAPAN), Public Relations and Information Strategy For immediate release: 19 March 2017

Okayama University research: Bioengineered tooth restoration in a large mammal

Researchers at Okayama University report in Scientific Reports successful tooth regeneration in a postnatal large-animal model. The approach used involves the autologous transplantation of bioengineered tooth germ into a canine jawbone; the in vivo artificially created tooth has the structure, composition and physiological characteristics of a natural tooth.

Conventional therapies for restoring the loss of a tooth due to e.g. caries, gum disease or injury essentially consist of replacing the tooth with artificial material or an osseointegrated dental implant. Whole-organ regeneration technology is a promising alternative approach: a new tooth is grown from bioengineered tooth germ transplanted into the jawbone. Takuo Kuboki from Okayama University and colleagues have now demonstrated successful functional tooth restoration via the regenerative method for a postnatal large-animal model (a beagle dog).

The researchers first tested whether bioengineered tooth germ does indeed lead to the formation of a proper tooth. They dissected embryonic tooth germ cells and tissues of a dog 55 days prior to birth, and then reconstructed bioengineered tooth germ by means of a technique known as the organ germ method. The germs were then transplanted into mice. In many cases Kuboki and colleagues were able to identify the necessary conditions the germ resulted in tooth-crown formation, featuring both the hard and soft tissues present in natural teeth, after several weeks.

The scientists then performed autologous transplantation experiments. Rather than relying on a donor, autologous treatments make use of an organism's own stem cells (undifferentiated cells that can develop into specialized cells), avoiding immunological rejection. Applying this to their canine model, Kuboki and co-workers extracted deciduous teeth from the jawbone of a 30-day old beagle dog. Tooth germ engineered from the dog's permanent tooth cell and tissue was then transplanted, after two days of cell culture, into the dog's mandible, resulting in tooth eruption 180 days later.

Micro-CT analysis showed that the developmental process of the bioengineered tooth's formation was practically identical to that of a natural tooth, and, by means of scanning electron microscopy and energy-dispersive X-ray spectroscopy, the bioengineered tooth was found to have the same structure and chemical composition of a natural one. Finally, the researchers demonstrated that the response of the regenerated tooth to a mechanical force was consistent with proper physiological functioning of the periodontal ligament (the tissue that connects the crown to the jawbone).

Regarding the future clinical application of the method to humans, the researchers pointed out that immature wisdom tooth germ would be a possible source of stem-cell germs, as it is available in the human postnatal jawbone. However, this would only pertain to younger people wisdom teeth mineralize after the age of 7; for elderly patients, other stem-cell sources would need to be identified. In any case, quoting Kuboki and colleagues, "this study highlights the feasibility of fully functional tooth restoration by autologous transplantation of bioengineered tooth germ".

Background Tooth structure and tooth loss remedies Teeth playing an essential role in the basic oral functions of mastication, swallowing and pronunciation comprise hard (such as enamel, dentin and cementum) and soft tissue (such as pulp and periodontal ligaments). As a remedy for tooth loss, fixed dental bridges or removable dentures made from artificial materials have been traditionally used, as well as osseointegrated dental implants: artificial teeth that are directly connected to the jawbone, without intervening soft tissue. Driven by recent advances in biomedical understanding and biotechnological engineering, regenerative technologies for the successful replacement of a lost tooth with uncompromised physiological tooth function such as the one now reported by Kuboki and colleagues are intensively researched today.

Donor-organ versus autologous transplantation The transplantation experiments carried out by the researchers are of the autologous type: a dog's own tooth germ stem cells were used to regenerate a missing tooth. An autologous transplantation avoids the potential problem of transplant rejection: when an organism receives a donor organ from another, genetically different organism, the former's immune system may attempt to destroy the transplant. Another complication can be graft-versus-host disease, caused by immune cells of the donated tissue recognizing the host as foreign and starting to attack host cells. It is therefore expected that future whole-tooth restoration in humans will be done by means of autologous transplantation techniques.

Organ germ method The approach of Kuboki and co-workers involves the bioengineered organ germ method, studied since about a decade ago. The method aims to regenerate ectodermal organs organs originating from the so-called ectoderm, the outer embryonic layer such as teeth, hairs and glands, by replicating the organ's developmental process starting from bioengineered organ germ. In a natural embryo, organ germ arises from the interaction between epithelium (the tissue at the outer layer of a body's surface) and mesenchyme (tissue sitting below the epithelium). Bioengineered organ germ is created by letting epithelial and mesenchymal tissue or cells interact.

Reference Mitsuaki Ono, Masamitsu Oshima, Miho Ogawa, Wataru Sonoyama, Emilio Satoshi Hara, Yasutaka Oida, Shigehiko Shinkawa, Ryu Nakajima, Atsushi Mine, Satoru Hayano, Satoshi Fukumoto, Shohei Kasugai, Akira Yamagushi, Takashi Tsuji & Takuo Kuboki. Practical whole-tooth restoration utilizing autologous bioengineered tooth germ transplantation in a postnatal canine model. Scientific Reports, 7, 44522. DOI : 10.1038/srep44522 (2017) http://www.nature.com/articles/srep44522

Reference (Okayama University e-Bulletin & OU-MRU) : Professor Kuboki's team e-Bulletin Vol.9Bio-hybrid implants: Restoring organ functions OU-MRU Vol.11Compound-protein combination shows promise for arthritis treatment OU-MRU Vol.19Study links signalling protein to osteoarthritis

About the author Vice-President and Professor Takuo Kuboki, D.D.S., Ph.D. Department of Oral Rehabilitation and Regenerative Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences http://www.okayama-u.ac.jp/user/implant/eng/index.html

Further information Okayama University Website: http://www.okayama-u.ac.jp/index_e.html Okayama Univ. e-Bulletin: http://www.okayama-u.ac.jp/user/kouhou/ebulletin/ About Okayama University (You Tube): https://www.youtube.com/watch?v=iDL1coqPRYI

Okayama University Medical Research Updates OU-MRU Vols 1 to 38. http://www.okayama-u.ac.jp/eng/research_highlights/index.html

For the original version on PRWeb visit: http://www.prweb.com/releases/2017/03/prweb14162998.htm

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Okayama University Research: Bioengineered Tooth Restoration in ... - Benzinga

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Dental caries – Wikipedia

Wednesday, December 7th, 2016

Dental caries, also known as tooth decay, cavities, or caries, is a breakdown of teeth due to activities of bacteria.[1] The cavities may be a number of different colors from yellow to black.[2] Symptoms may include pain and difficulty with eating.[2][3] Complications may include inflammation of the tissue around the tooth, tooth loss, and infection or abscess formation.[2][4]

The cause of caries is bacterial breakdown of the hard tissues of the teeth (enamel, dentin and cementum). This occurs due to acid made from food debris or sugar on the tooth surface. Simple sugars in food are these bacteria's primary energy source and thus a diet high in simple sugar is a risk factor. If mineral breakdown is greater than build up from sources such as saliva, caries results. Risk factors include conditions that result in less saliva such as: diabetes mellitus, Sjogren's syndrome and some medications. Medications that decrease saliva production include antihistamines and antidepressants.[5] Caries is also associated with poverty, poor cleaning of the mouth, and receding gums resulting in exposure of the roots of the teeth.[1][6]

Prevention includes: regular cleaning of the teeth, a diet low in sugar, and small amounts of fluoride.[3][5] Brushing the teeth twice per day and flossing between the teeth once a day is recommended by many.[1][5] Fluoride may be from water, salt or toothpaste among other sources.[3] Treating a mother's dental caries may decrease the risk in her children by decreasing the numbers of certain bacteria.[5] Screening can result in earlier detection.[1] Depending on the extent of destruction, various treatments can be used to restore the tooth to proper function or the tooth may be removed.[1] There is no known method to grow back large amounts of tooth.[7] The availability of treatment is often poor in the developing world.[3]Paracetamol (acetaminophen) or ibuprofen may be taken for pain.[1]

Worldwide, approximately 2.43billion people (36% of the population) have dental caries in their permanent teeth.[8] The World Health Organization estimates that nearly all adults have dental caries at some point in time.[3] In baby teeth it affects about 620million people or 9% of the population.[8] They have become more common in both children and adults in recent years.[9] The disease is most common in the developed world due to greater simple sugar consumption and less common in the developing world.[1] Caries is Latin for "rottenness".[4]

A person experiencing caries may not be aware of the disease.[10] The earliest sign of a new carious lesion is the appearance of a chalky white spot on the surface of the tooth, indicating an area of demineralization of enamel. This is referred to as a white spot lesion, an incipient carious lesion or a "microcavity".[11] As the lesion continues to demineralize, it can turn brown but will eventually turn into a cavitation ("cavity"). Before the cavity forms, the process is reversible, but once a cavity forms, the lost tooth structure cannot be regenerated. A lesion that appears dark brown and shiny suggests dental caries were once present but the demineralization process has stopped, leaving a stain. Active decay is lighter in color and dull in appearance.[12]

As the enamel and dentin are destroyed, the cavity becomes more noticeable. The affected areas of the tooth change color and become soft to the touch. Once the decay passes through enamel, the dentinal tubules, which have passages to the nerve of the tooth, become exposed, resulting in pain that can be transient, temporarily worsening with exposure to heat, cold, or sweet foods and drinks.[13] A tooth weakened by extensive internal decay can sometimes suddenly fracture under normal chewing forces. When the decay has progressed enough to allow the bacteria to overwhelm the pulp tissue in the center of the tooth, a toothache can result and the pain will become more constant. Death of the pulp tissue and infection are common consequences. The tooth will no longer be sensitive to hot or cold, but can be very tender to pressure.

Dental caries can also cause bad breath and foul tastes.[14] In highly progressed cases, infection can spread from the tooth to the surrounding soft tissues. Complications such as cavernous sinus thrombosis and Ludwig angina can be life-threatening.[15][16][17]

Four things are required for caries formation: a tooth surface (enamel or dentin), caries-causing bacteria, fermentable carbohydrates (such as sucrose), and time.[18] This involves adherence of food to the teeth and acid creation by the bacteria that makes up the dental plaque.[19] However, these four criteria are not always enough to cause the disease and a sheltered environment promoting development of a cariogenic biofilm is required. The caries disease process does not have an inevitable outcome, and different individuals will be susceptible to different degrees depending on the shape of their teeth, oral hygiene habits, and the buffering capacity of their saliva. Dental caries can occur on any surface of a tooth that is exposed to the oral cavity, but not the structures that are retained within the bone.[20]

Tooth decay is caused by biofilm (dental plaque) lying on the teeth and maturing to become cariogenic (causing decay). Certain bacteria in the biofilm produce acid in the presence of fermentable carbohydrates such as sucrose, fructose, and glucose.[21][22]

Caries occur more often in people from the lower end of the socioeconomic scale than people from the upper end of the socioeconomic scale.[23]

The most common bacteria associated with dental cavities are the mutans streptococci, most prominently Streptococcus mutans and Streptococcus sobrinus, and lactobacilli. However, cariogenic bacteria (the ones that can cause the disease) are present in dental plaque, but they are usually in too low concentrations to cause problems unless there is a shift in the balance.[24] This is driven by local environmental change, such as frequent sugar, no biofilm removal (a lack of toothbrushing).[25] If left untreated, the disease can lead to pain, tooth loss and infection.[26]

The mouth contains a wide variety of oral bacteria, but only a few specific species of bacteria are believed to cause dental caries: Streptococcus mutans and Lactobacillus species among them. These organisms can produce high levels of lactic acid following fermentation of dietary sugars, and are resistant to the adverse effects of low pH, properties essential for cariogenic bacteria.[21] As the cementum of root surfaces is more easily demineralized than enamel surfaces, a wider variety of bacteria can cause root caries, including Lactobacillus acidophilus, Actinomyces spp., Nocardia spp., and Streptococcus mutans. Bacteria collect around the teeth and gums in a sticky, creamy-coloured mass called plaque, which serves as a biofilm. Some sites collect plaque more commonly than others, for example sites with a low rate of salivary flow (molar fissures). Grooves on the occlusal surfaces of molar and premolar teeth provide microscopic retention sites for plaque bacteria, as do the interproximal sites. Plaque may also collect above or below the gingiva, where it is referred to as supra- or sub-gingival plaque, respectively.

These bacterial strains, most notably S. mutans, can be inherited by a child from a caretaker's kiss or through feeding premasticated.[27]

Bacteria in a person's mouth convert glucose, fructose, and most commonly sucrose (table sugar) into acids such as lactic acid through a glycolytic process called fermentation.[22] If left in contact with the tooth, these acids may cause demineralization, which is the dissolution of its mineral content. The process is dynamic, however, as remineralization can also occur if the acid is neutralized by saliva or mouthwash. Fluoride toothpaste or dental varnish may aid remineralization.[28] If demineralization continues over time, enough mineral content may be lost so that the soft organic material left behind disintegrates, forming a cavity or hole. The impact such sugars have on the progress of dental caries is called cariogenicity. Sucrose, although a bound glucose and fructose unit, is in fact more cariogenic than a mixture of equal parts of glucose and fructose. This is due to the bacteria utilising the energy in the saccharide bond between the glucose and fructose subunits. S.mutans adheres to the biofilm on the tooth by converting sucrose into an extremely adhesive substance called dextran polysaccharide by the enzyme dextransucranase.[29]

The frequency with which teeth are exposed to cariogenic (acidic) environments affects the likelihood of caries development.[30] After meals or snacks, the bacteria in the mouth metabolize sugar, resulting in an acidic by-product that decreases pH. As time progresses, the pH returns to normal due to the buffering capacity of saliva and the dissolved mineral content of tooth surfaces. During every exposure to the acidic environment, portions of the inorganic mineral content at the surface of teeth dissolve and can remain dissolved for two hours.[31] Since teeth are vulnerable during these acidic periods, the development of dental caries relies heavily on the frequency of acid exposure.

The carious process can begin within days of a tooth's erupting into the mouth if the diet is sufficiently rich in suitable carbohydrates. Evidence suggests that the introduction of fluoride treatments has slowed the process.[32] Proximal caries take an average of four years to pass through enamel in permanent teeth. Because the cementum enveloping the root surface is not nearly as durable as the enamel encasing the crown, root caries tends to progress much more rapidly than decay on other surfaces. The progression and loss of mineralization on the root surface is 2.5 times faster than caries in enamel. In very severe cases where oral hygiene is very poor and where the diet is very rich in fermentable carbohydrates, caries may cause cavities within months of tooth eruption. This can occur, for example, when children continuously drink sugary drinks from baby bottles (see later discussion).

There are certain diseases and disorders affecting teeth that may leave an individual at a greater risk for cavities.

Molar incisor hypomineralization, which is increasing in prevalence.[33] It is caused by systemic factors such as high levels of dioxins or polychlorinated biphenyl (PCB) in the mothers milk, premature birth and oxygen deprivation at birth, and certain disorders during the childs first 3 years such as such as mumps, diphtheria, scarlet fever, measles, hypoparathyroidism, malnutrition, malabsorption, hypovitaminosis D, chronic respiratory diseases, or undiagnosed and untreated coeliac disease, which usually presents with mild or absent gastrointestinal symptoms.[33][34][35][36][37][38]

Amelogenesis imperfecta, which occurs in between 1 in 718 and 1 in 14,000 individuals, is a disease in which the enamel does not fully form or forms in insufficient amounts and can fall off a tooth.[39] In both cases, teeth may be left more vulnerable to decay because the enamel is not able to protect the tooth.[40]

In most people, disorders or diseases affecting teeth are not the primary cause of dental caries. Approximately 96% of tooth enamel is composed of minerals.[41] These minerals, especially hydroxyapatite, will become soluble when exposed to acidic environments. Enamel begins to demineralize at a pH of 5.5.[42]Dentin and cementum are more susceptible to caries than enamel because they have lower mineral content.[43] Thus, when root surfaces of teeth are exposed from gingival recession or periodontal disease, caries can develop more readily. Even in a healthy oral environment, however, the tooth is susceptible to dental caries.

The evidence for linking malocclusion and/or crowding to dental caries is weak;[44][45] however, the anatomy of teeth may affect the likelihood of caries formation. Where the deep developmental grooves of teeth are more numerous and exaggerated, pit and fissure caries is more likely to develop (see next section). Also, caries is more likely to develop when food is trapped between teeth.

Reduced salivary flow rate is associated with increased caries since the buffering capability of saliva is not present to counterbalance the acidic environment created by certain foods. As a result, medical conditions that reduce the amount of saliva produced by salivary glands, in particular the submandibular gland and parotid gland, are likely to lead to dry mouth and thus to widespread tooth decay. Examples include Sjgren's syndrome, diabetes mellitus, diabetes insipidus, and sarcoidosis.[46] Medications, such as antihistamines and antidepressants, can also impair salivary flow. Stimulants, most notoriously methylamphetamine, also occlude the flow of saliva to an extreme degree ("meth mouth"). Tetrahydrocannabinol (THC), the active chemical substance in cannabis, also causes a nearly complete occlusion of salivation, known in colloquial terms as "cotton mouth". Moreover, 63% of the most commonly prescribed medications in the United States list dry mouth as a known side-effect.[46] Radiation therapy of the head and neck may also damage the cells in salivary glands, somewhat increasing the likelihood of caries formation.[47][48]

Susceptibility to caries can be related to altered metabolism in the tooth, in particular to fluid flow in the dentin. Experiments on rats have shown that a high-sucrose, cariogenic diet "significantly suppresses the rate of fluid motion" in dentin.[49]

The use of tobacco may also increase the risk for caries formation. Some brands of smokeless tobacco contain high sugar content, increasing susceptibility to caries.[50] Tobacco use is a significant risk factor for periodontal disease, which can cause the gingiva to recede.[51] As the gingiva loses attachment to the teeth due to gingival recession, the root surface becomes more visible in the mouth. If this occurs, root caries is a concern since the cementum covering the roots of teeth is more easily demineralized by acids than enamel.[52] Currently, there is not enough evidence to support a causal relationship between smoking and coronal caries, but evidence does suggest a relationship between smoking and root-surface caries.[53] Exposure of children to secondhand tobacco smoke is associated with tooth decay.[54]

Intrauterine and neonatal lead exposure promote tooth decay.[55][56][57][58][59][60][61] Besides lead, all atoms with electrical charge and ionic radius similar to bivalent calcium,[62] such as cadmium, mimic the calcium ion and therefore exposure to them may promote tooth decay.[63]

Poverty is also a significant social determinant for oral health.[64] Dental caries have been linked with lower socio-economic status and can be considered a disease of poverty.[65]

Forms are available for risk assessment for caries when treating dental cases; this system using the evidence-based Caries Management by Risk Assessment (CAMBRA).[66] It is still unknown if the identification of high-risk individuals can lead to more effective long-term patient management that prevents caries initiation and arrests or reverses the progression of lesions.[67]

Saliva also contains iodine and EGF. EGF results effective in cellular proliferation, differentiation and survival.[68] Salivary EGF, which seems also regulated by dietary inorganic iodine, plays an important physiological role in the maintenance of oral (and gastro-oesophageal) tissue integrity, and, on the other hand, iodine is effective in prevention of dental caries and oral health.[69]

Teeth are bathed in saliva and have a coating of bacteria on them (biofilm) that continually forms. The minerals in the hard tissues of the teeth (enamel, dentin and cementum) are constantly undergoing processes of demineralization and remineralisation. Dental caries results when the demineralization rate is faster than the remineralisation and there is net mineral loss. This happens when there is an ecologic shift within the dental biofilm, from a balanced population of micro-organisms to a population that produce acids and can survive in an acid environment.[70]

Enamel is a highly mineralized acellular tissue, and caries act upon it through a chemical process brought on by the acidic environment produced by bacteria. As the bacteria consume the sugar and use it for their own energy, they produce lactic acid. The effects of this process include the demineralization of crystals in the enamel, caused by acids, over time until the bacteria physically penetrate the dentin. Enamel rods, which are the basic unit of the enamel structure, run perpendicularly from the surface of the tooth to the dentin. Since demineralization of enamel by caries, in general, follows the direction of the enamel rods, the different triangular patterns between pit and fissure and smooth-surface caries develop in the enamel because the orientation of enamel rods are different in the two areas of the tooth.[71]

As the enamel loses minerals, and dental caries progresses, the enamel develop several distinct zones, visible under a light microscope. From the deepest layer of the enamel to the enamel surface, the identified areas are the: translucent zone, dark zones, body of the lesion, and surface zone.[72] The translucent zone is the first visible sign of caries and coincides with a one to two percent loss of minerals.[73] A slight remineralization of enamel occurs in the dark zone, which serves as an example of how the development of dental caries is an active process with alternating changes.[74] The area of greatest demineralization and destruction is in the body of the lesion itself. The surface zone remains relatively mineralized and is present until the loss of tooth structure results in a cavitation.

Unlike enamel, the dentin reacts to the progression of dental caries. After tooth formation, the ameloblasts, which produce enamel, are destroyed once enamel formation is complete and thus cannot later regenerate enamel after its destruction. On the other hand, dentin is produced continuously throughout life by odontoblasts, which reside at the border between the pulp and dentin. Since odontoblasts are present, a stimulus, such as caries, can trigger a biologic response. These defense mechanisms include the formation of sclerotic and tertiary dentin.[75]

In dentin from the deepest layer to the enamel, the distinct areas affected by caries are the advancing front, the zone of bacterial penetration, and the zone of destruction.[71] The advancing front represents a zone of demineralised dentin due to acid and has no bacteria present. The zones of bacterial penetration and destruction are the locations of invading bacteria and ultimately the decomposition of dentin. The zone of destruction has a more mixed bacterial population where proteolytic enzymes have destroyed the organic matrix. The innermost dentin caries has been reversibly attacked because the collage matrix is not severely damaged, giving it potential for repair. The outer more superficial zone is highly infected with proteolytic degradation of the collagen matrix and as a result the dentin is irreversibly demineralised.[citation needed]

The structure of dentin is an arrangement of microscopic channels, called dentinal tubules, which radiate outward from the pulp chamber to the exterior cementum or enamel border.[76] The diameter of the dentinal tubules is largest near the pulp (about 2.5m) and smallest (about 900nm) at the junction of dentin and enamel.[77] The carious process continues through the dentinal tubules, which are responsible for the triangular patterns resulting from the progression of caries deep into the tooth. The tubules also allow caries to progress faster.

In response, the fluid inside the tubules brings immunoglobulins from the immune system to fight the bacterial infection. At the same time, there is an increase of mineralization of the surrounding tubules.[78] This results in a constriction of the tubules, which is an attempt to slow the bacterial progression. In addition, as the acid from the bacteria demineralizes the hydroxyapatite crystals, calcium and phosphorus are released, allowing for the precipitation of more crystals which fall deeper into the dentinal tubule. These crystals form a barrier and slow the advancement of caries. After these protective responses, the dentin is considered sclerotic.

According to hydrodynamic theory, fluids within dentinal tubules are believed to be the mechanism by which pain receptors are triggered within the pulp of the tooth.[79] Since sclerotic dentin prevents the passage of such fluids, pain that would otherwise serve as a warning of the invading bacteria may not develop at first. Consequently, dental caries may progress for a long period of time without any sensitivity of the tooth, allowing for greater loss of tooth structure.[citation needed]

In response to dental caries, there may be production of more dentin toward the direction of the pulp. This new dentin is referred to as tertiary dentin.[77] Tertiary dentin is produced to protect the pulp for as long as possible from the advancing bacteria. As more tertiary dentin is produced, the size of the pulp decreases. This type of dentin has been subdivided according to the presence or absence of the original odontoblasts.[80] If the odontoblasts survive long enough to react to the dental caries, then the dentin produced is called "reactionary" dentin. If the odontoblasts are killed, the dentin produced is called "reparative" dentin.

In the case of reparative dentin, other cells are needed to assume the role of the destroyed odontoblasts. Growth factors, especially TGF-,[80] are thought to initiate the production of reparative dentin by fibroblasts and mesenchymal cells of the pulp.[81] Reparative dentin is produced at an average of 1.5m/day, but can be increased to 3.5m/day. The resulting dentin contains irregularly shaped dentinal tubules that may not line up with existing dentinal tubules. This diminishes the ability for dental caries to progress within the dentinal tubules.

The incidence of cemental caries increases in older adults as gingival recession occurs from either trauma or periodontal disease. It is a chronic condition that forms a large, shallow lesion and slowly invades first the roots cementum and then dentin to cause a chronic infection of the pulp (see further discussion under classification by affected hard tissue). Because dental pain is a late finding, many lesions are not detected early, resulting in restorative challenges and increased tooth loss.[82]

The presentation of caries is highly variable. However, the risk factors and stages of development are similar. Initially it may appear as a small chalky area (smooth surface caries), which may eventually develop into a large cavitation. Sometimes caries may be directly visible. However other methods of detection such as X-rays are used for less visible areas of teeth and to judge the extent of destruction. Lasers for detecting caries allow detection without ionizing radiation and are now used for detection of interproximal decay (between the teeth). Disclosing solutions are also used during tooth restoration to minimize the chance of recurrence.[citation needed]

Primary diagnosis involves inspection of all visible tooth surfaces using a good light source, dental mirror and explorer. Dental radiographs (X-rays) may show dental caries before it is otherwise visible, in particular caries between the teeth. Large areas of dental caries are often apparent to the naked eye, but smaller lesions can be difficult to identify. Visual and tactile inspection along with radiographs are employed frequently among dentists, in particular to diagnose pit and fissure caries.[84] Early, uncavitated caries is often diagnosed by blowing air across the suspect surface, which removes moisture and changes the optical properties of the unmineralized enamel.

Some dental researchers have cautioned against the use of dental explorers to find caries,[85] in particular sharp ended explorers. In cases where a small area of tooth has begun demineralizing but has not yet cavitated, the pressure from the dental explorer could cause a cavity. Since the carious process is reversible before a cavity is present, it may be possible to arrest the caries with fluoride and remineralize the tooth surface. When a cavity is present, a restoration will be needed to replace the lost tooth structure.

At times, pit and fissure caries may be difficult to detect. Bacteria can penetrate the enamel to reach dentin, but then the outer surface may remineralize, especially if fluoride is present.[86] These caries, sometimes referred to as "hidden caries", will still be visible on X-ray radiographs, but visual examination of the tooth would show the enamel intact or minimally perforated.

The differential diagnosis for dental caries includes dental fluorosis and developmental defects of the tooth including hypomineralization of the tooth and hypoplasia of the tooth.[87]

The early carious lesion is characterized by demineralization of the tooth surface, altering the tooth's optical properties. Technology utilizing laser speckle image (LSI) techniques may provide a diagnostic aid to detect early carious lesions.[83]

Caries can be classified by location, etiology, rate of progression, and affected hard tissues.[88] These forms of classification can be used to characterize a particular case of tooth decay in order to more accurately represent the condition to others and also indicate the severity of tooth destruction. In some instances, caries is described in other ways that might indicate the cause. The G.V. Black classification is as follows:

Early childhood caries (ECC), also known as "baby bottle caries," "baby bottle tooth decay" or "bottle rot," is a pattern of decay found in young children with their deciduous (baby) teeth. The teeth most likely affected are the maxillary anterior teeth, but all teeth can be affected.[89] The name for this type of caries comes from the fact that the decay usually is a result of allowing children to fall asleep with sweetened liquids in their bottles or feeding children sweetened liquids multiple times during the day.[90]

Another pattern of decay is "rampant caries", which signifies advanced or severe decay on multiple surfaces of many teeth.[91] Rampant caries may be seen in individuals with xerostomia, poor oral hygiene, stimulant use (due to drug-induced dry mouth[92]), and/or large sugar intake. If rampant caries is a result of previous radiation to the head and neck, it may be described as radiation-induced caries. Problems can also be caused by the self-destruction of roots and whole tooth resorption when new teeth erupt or later from unknown causes.

Children at 612 months are at increased risk of developing dental caries. For other kids aged 1218 months, dental caries develop on primary teeth and approximately twice yearly for permanent teeth.[93]

Temporal descriptions can be applied to caries to indicate the progression rate and previous history. "Acute" signifies a quickly developing condition, whereas "chronic" describes a condition that has taken an extended time to develop, in which thousands of meals and snacks, many causing some acid demineralization that is not remineralized, eventually result in cavities.

Recurrent caries, also described as secondary, are caries that appear at a location with a previous history of caries. This is frequently found on the margins of fillings and other dental restorations. On the other hand, incipient caries describes decay at a location that has not experienced previous decay. Arrested caries describes a lesion on a tooth that was previously demineralized but was remineralized before causing a cavitation. Fluoride treatment can help recalcification of tooth enamel as well as use of amorphous calcium phosphate.

Depending on which hard tissues are affected, it is possible to describe caries as involving enamel, dentin, or cementum. Early in its development, caries may affect only enamel. Once the extent of decay reaches the deeper layer of dentin, the term "dentinal caries" is used. Since cementum is the hard tissue that covers the roots of teeth, it is not often affected by decay unless the roots of teeth are exposed to the mouth. Although the term "cementum caries" may be used to describe the decay on roots of teeth, very rarely does caries affect the cementum alone. Roots have a very thin layer of cementum over a large layer of dentin, and thus most caries affecting cementum also affects dentin.[citation needed]

Personal hygiene care consists of proper brushing and flossing daily. The purpose of oral hygiene is to minimize any etiologic agents of disease in the mouth. The primary focus of brushing and flossing is to remove and prevent the formation of plaque or dental biofilm. Plaque consists mostly of bacteria.[94] As the amount of bacterial plaque increases, the tooth is more vulnerable to dental caries when carbohydrates in the food are left on teeth after every meal or snack. A toothbrush can be used to remove plaque on accessible surfaces, but not between teeth or inside pits and fissures on chewing surfaces. When used correctly, dental floss removes plaque from areas that could otherwise develop proximal caries but only if the depth of sulcus has not been compromised. Other adjunct oral hygiene aids include interdental brushes, water picks, and mouthwashes.

However oral hygiene is probably more effective at preventing gum disease (periodontal disease) than tooth decay. Food is forced inside pits and fissures under chewing pressure, leading to carbohydrate-fueled acid demineralisation where the brush, fluoride toothpaste, and saliva have no access to remove trapped food, neutralise acid, or remineralise demineralised tooth like on other more accessible tooth surfaces. (Occlusal caries accounts for between 80 and 90% of caries in children (Weintraub, 2001).) Chewing fibre like celery after eating forces saliva inside trapped food to dilute any carbohydrate like sugar, neutralise acid and remineralise demineralised tooth. The teeth at highest risk for carious lesions are the permanent first and second molars due to length of time in oral cavity and presence of complex surface anatomy.

Professional hygiene care consists of regular dental examinations and professional prophylaxis (cleaning). Sometimes, complete plaque removal is difficult, and a dentist or dental hygienist may be needed. Along with oral hygiene, radiographs may be taken at dental visits to detect possible dental caries development in high risk areas of the mouth (e.g. "bitewing" X-rays which visualize the crowns of the back teeth).

For dental health, frequency of sugar intake is more important than the amount of sugar consumed.[30] In the presence of sugar and other carbohydrates, bacteria in the mouth produce acids that can demineralize enamel, dentin, and cementum. The more frequently teeth are exposed to this environment, the more likely dental caries is to occur. Therefore, minimizing snacking is recommended, since snacking creates a continuous supply of nutrition for acid-creating bacteria in the mouth. Also, chewy and sticky foods (such as candy, cookies, potato chips, and crackers) tend to adhere to teeth longer. However, dried fruits such as raisins and fresh fruit such as apples and bananas disappear from the mouth quickly, and do not appear to be a risk factor.[95] For children, the American Dental Association and the European Academy of Paediatric Dentistry recommend limiting the frequency of consumption of drinks with sugar, and not giving baby bottles to infants during sleep (see earlier discussion).[96][97] Mothers are also recommended to avoid sharing utensils and cups with their infants to prevent transferring bacteria from the mother's mouth.[98]

It has been found that milk and certain kinds of cheese like cheddar cheese can help counter tooth decay if eaten soon after the consumption of foods potentially harmful to teeth.[30] Also, chewing gum containing xylitol (a sugar alcohol) is widely used to protect teeth in many countries now. Xylitol's effect on reducing dental biofilm is, it is presumed, due to bacteria's inability to utilize it like other sugars.[99] Chewing and stimulation of flavor receptors on the tongue are also known to increase the production and release of saliva, which contains natural buffers to prevent the lowering of pH in the mouth to the point where enamel may become demineralized.[100]

The use of dental sealants is a means of prevention.[101] A sealant is a thin plastic-like coating applied to the chewing surfaces of the molars to prevent food from being trapped inside pits and fissures. This deprives resident plaque bacteria of carbohydrate, preventing the formation of pit and fissure caries. Sealants are usually applied on the teeth of children, as soon as the teeth erupt but adults are receiving them if not previously performed. Sealants can wear out and fail to prevent access of food and plaque bacteria inside pits and fissures and need to be replaced so they must be checked regularly by dental professionals.

Calcium, as found in food such as milk and green vegetables, is often recommended to protect against dental caries. Fluoride helps prevent decay of a tooth by binding to the hydroxyapatite crystals in enamel.[102] The incorporated fluorine makes enamel more resistant to demineralization and, thus, resistant to decay.[103] Topical fluoride is more highly recommended than systemic intake such as by tablets or drops to protect the surface of the teeth. This may include a fluoride toothpaste or mouthwash or varnish.[104] After brushing with fluoride toothpaste, rinsing should be avoided and the excess spat out.[105] Many dental professionals include application of topical fluoride solutions as part of routine visits and recommend the use of xylitol and amorphous calcium phosphate products. Silver diamine fluoride may work better than fluoride varnish to prevent cavities.[106] Water fluoridation also lowers the risk of tooth decay.[107]

An oral health assessment carried out before a child reaches the age of one may help with management of caries. The oral health assessment should include checking the childs history, a clinical examination, checking the risk of caries in the child including the state of their occlusion and assessing how well equipped the childs parent or carer is to help the child prevent caries.[108] In order to further increase a childs cooperation in caries management, good communication by the dentist and the rest of the staff of a dental practice should be used. This communication can be improved by calling the child by their name, using eye contact and including them in any conversation about their treatment.[108]

Vaccines are also under development.[109]

Most importantly, whether the carious lesion is cavitated or noncavitated dictates the management. Clinical assessment of whether the lesion is active or arrested is also important. Noncavitated lesions can be arrested and remineralization can occur under the right conditions. However, this may require extensive changes to the diet (reduction in frequency of refined sugars), improved oral hygiene (toothbrushing twice per day with fluoride toothpaste and daily flossing), and regular application of topical fluoride. Such management of a carious lesion is termed "non-operative" since no drilling is carried out on the tooth. Non-operative treatment requires excellent understanding and motivation from the individual, otherwise the decay will continue.

Once a lesion has cavitated, especially if dentin is involved, remineralization is much more difficult and a dental restoration is usually indicated ("operative treatment"). Before a restoration can be placed, all of the decay must be removed otherwise it will continue to progress underneath the filling. Sometimes a small amount of decay can be left if it is entombed and there is a seal which isolates the bacteria from their substrate. This can be likened to placing a glass container over a candle, which burns itself out once the oxygen is used up. Techniques such as stepwise caries removal are designed to avoid exposure of the dental pulp and overall reduction of the amount of tooth substance which requires removal before the final filling is placed. Often enamel which overlies decayed dentin must also be removed as it is unsupported and susceptible to fracture. The modern decision-making process with regards the activity of the lesion, and whether it is cavitated, is summarized in the table.[110]

Destroyed tooth structure does not fully regenerate, although remineralization of very small carious lesions may occur if dental hygiene is kept at optimal level.[13] For the small lesions, topical fluoride is sometimes used to encourage remineralization. For larger lesions, the progression of dental caries can be stopped by treatment. The goal of treatment is to preserve tooth structures and prevent further destruction of the tooth. Aggressive treatment, by filling, of incipient carious lesions, places where there is superficial damage to the enamel, is controversial as they may heal themselves, while once a filling is performed it will eventually have to be redone and the site serves as a vulnerable site for further decay.[11]

In general, early treatment is quicker and less expensive than treatment of extensive decay. Local anesthetics, nitrous oxide ("laughing gas"), or other prescription medications may be required in some cases to relieve pain during or following treatment or to relieve anxiety during treatment.[111] A dental handpiece ("drill") is used to remove large portions of decayed material from a tooth. A spoon, a dental instrument used to carefully remove decay, is sometimes employed when the decay in dentin reaches near the pulp.[112] Some dentists remove dental caries using a laser rather than the traditional dental drill. A Cochrane review of this technique looked at Er:YAG (erbium-doped yttrium aluminium garnet), Er,Cr:YSGG (erbium, chromium: yttrium-scandium-gallium-garnet) and Nd:YAG (neodymium-doped yttrium aluminium garnet) lasers and found that although people treated with lasers (compared to a conventional dental "drill") experienced less pain and had a lesser need for dental anaesthesia, that overall there was little difference in caries removal.[113] Once the caries is removed, the missing tooth structure requires a dental restoration of some sort to return the tooth to function and aesthetic condition.

Restorative materials include dental amalgam, composite resin, porcelain, and gold.[114] Composite resin and porcelain can be made to match the color of a patient's natural teeth and are thus used more frequently when aesthetics are a concern. Composite restorations are not as strong as dental amalgam and gold; some dentists consider the latter as the only advisable restoration for posterior areas where chewing forces are great.[115] When the decay is too extensive, there may not be enough tooth structure remaining to allow a restorative material to be placed within the tooth. Thus, a crown may be needed. This restoration appears similar to a cap and is fitted over the remainder of the natural crown of the tooth. Crowns are often made of gold, porcelain, or porcelain fused to metal.

For children, preformed crowns are available to place over the tooth. These are usually made of metal (usually stainless steel but increasingly there are aesthetic materials). Traditionally teeth are shaved down to make room for the crown but, more recently, stainless steel crowns have been used to seal decay into the tooth and stop it progressing. This is known as the Hall Technique and works by depriving the bacteria in the decay of nutrients and making their environment less favorable for them. It is a minimally invasive method of managing decay in children and does not require local anesthetic injections in the mouth.

In certain cases, endodontic therapy may be necessary for the restoration of a tooth.[116] Endodontic therapy, also known as a "root canal", is recommended if the pulp in a tooth dies from infection by decay-causing bacteria or from trauma. In root canal therapy, the pulp of the tooth, including the nerve and vascular tissues, is removed along with decayed portions of the tooth. The canals are instrumented with endodontic files to clean and shape them, and they are then usually filled with a rubber-like material called gutta percha.[117] The tooth is filled and a crown can be placed. Upon completion of root canal therapy, the tooth is non-vital, as it is devoid of any living tissue.

An extraction can also serve as treatment for dental caries. The removal of the decayed tooth is performed if the tooth is too far destroyed from the decay process to effectively restore the tooth. Extractions are sometimes considered if the tooth lacks an opposing tooth or will probably cause further problems in the future, as may be the case for wisdom teeth.[118] Extractions may also be preferred by people unable or unwilling to undergo the expense or difficulties in restoring the tooth.

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Worldwide, approximately 2.43billion people (36% of the population) have dental caries in their permanent teeth.[8] In baby teeth it affects about 620million people or 9% of the population.[8] The disease is most common in Latin American countries, countries in the Middle East, and South Asia, and least prevalent in China.[120] In the United States, dental caries is the most common chronic childhood disease, being at least five times more common than asthma.[121] It is the primary pathological cause of tooth loss in children.[122] Between 29% and 59% of adults over the age of 50 experience caries.[123]

The number of cases has decreased in some developed countries, and this decline is usually attributed to increasingly better oral hygiene practices and preventive measures such as fluoride treatment.[124] Nonetheless, countries that have experienced an overall decrease in cases of tooth decay continue to have a disparity in the distribution of the disease.[123] Among children in the United States and Europe, twenty percent of the population endures sixty to eighty percent of cases of dental caries.[125] A similarly skewed distribution of the disease is found throughout the world with some children having none or very few caries and others having a high number.[123]Australia, Nepal, and Sweden (where children receive dental care paid for by the government) have a low incidence of cases of dental caries among children, whereas cases are more numerous in Costa Rica and Slovakia.[126]

The classic DMF (decay/missing/filled) index is one of the most common methods for assessing caries prevalence as well as dental treatment needs among populations. This index is based on in-field clinical examination of individuals by using a probe, mirror and cotton rolls. Because the DMF index is done without X-ray imaging, it underestimates real caries prevalence and treatment needs.[86]

Bacteria typically associated with dental caries have been isolated from vaginal samples from females who have bacterial vaginosis.[127]

There is a long history of dental caries. Over a million years ago, hominins such as Australopithecus suffered from cavities.[128] The largest increases in the prevalence of caries have been associated with dietary changes.[128][129] Archaeological evidence shows that tooth decay is an ancient disease dating far into prehistory. Skulls dating from a million years ago through the neolithic period show signs of caries, including those from the Paleolithic and Mesolithic ages.[130] The increase of caries during the neolithic period may be attributed to the increased consumption of plant foods containing carbohydrates.[131] The beginning of rice cultivation in South Asia is also believed to have caused an increase in caries, although there is also some evidence from sites in Thailand, such as Khok Phanom Di, that shows a decrease in overall percentage of dental caries with the increase in dependence on rice agriculture.[132]

A Sumerian text from 5000 BC describes a "tooth worm" as the cause of caries.[133] Evidence of this belief has also been found in India, Egypt, Japan, and China.[129] Unearthed ancient skulls show evidence of primitive dental work. In Pakistan, teeth dating from around 5500 BC to 7000 BC show nearly perfect holes from primitive dental drills.[134] The Ebers Papyrus, an Egyptian text from 1550 BC, mentions diseases of teeth.[133] During the Sargonid dynasty of Assyria during 668 to 626 BC, writings from the king's physician specify the need to extract a tooth due to spreading inflammation.[129] In the Roman Empire, wider consumption of cooked foods led to a small increase in caries prevalence.[125] The Greco-Roman civilization, in addition to the Egyptian, had treatments for pain resulting from caries.[129]

The rate of caries remained low through the Bronze Age and Iron Age, but sharply increased during the Middle Ages.[128] Periodic increases in caries prevalence had been small in comparison to the 1000 AD increase, when sugar cane became more accessible to the Western world. Treatment consisted mainly of herbal remedies and charms, but sometimes also included bloodletting.[135] The barber surgeons of the time provided services that included tooth extractions.[129] Learning their training from apprenticeships, these health providers were quite successful in ending tooth pain and likely prevented systemic spread of infections in many cases. Among Roman Catholics, prayers to Saint Apollonia, the patroness of dentistry, were meant to heal pain derived from tooth infection.[136]

There is also evidence of caries increase in North American Indians after contact with colonizing Europeans. Before colonization, North American Indians subsisted on hunter-gatherer diets, but afterwards there was a greater reliance on maize agriculture, which made these groups more susceptible to caries.[128]

During the European Age of Enlightenment, the belief that a "tooth worm" caused caries was also no longer accepted in the European medical community.[137]Pierre Fauchard, known as the father of modern dentistry, was one of the first to reject the idea that worms caused tooth decay and noted that sugar was detrimental to the teeth and gingiva.[138] In 1850, another sharp increase in the prevalence of caries occurred and is believed to be a result of widespread diet changes.[129] Prior to this time, cervical caries was the most frequent type of caries, but increased availability of sugar cane, refined flour, bread, and sweetened tea corresponded with a greater number of pit and fissure caries.

In the 1890s, W.D. Miller conducted a series of studies that led him to propose an explanation for dental caries that was influential for current theories. He found that bacteria inhabited the mouth and that they produced acids that dissolved tooth structures when in the presence of fermentable carbohydrates.[139] This explanation is known as the chemoparasitic caries theory.[140] Miller's contribution, along with the research on plaque by G.V. Black and J.L. Williams, served as the foundation for the current explanation of the etiology of caries.[129] Several of the specific strains of lactobacilli were identified in 1921 by Fernando E. Rodriguez Vargas.

In 1924 in London, Killian Clarke described a spherical bacterium in chains isolated from carious lesions which he called Streptococcus mutans. Although Clarke proposed that this organism was the cause of caries, the discovery was not followed up. Later, in the 1950s in the USA, Keyes and Fitzgerald working with hamsters showed that caries was transmissible and caused by an acid-producing Streptococcus. It was not until the late 1960s that it became generally accepted that the Streptococcus isolated from hamster caries was the same as S. mutans described by Clarke.[141]

Tooth decay has been present throughout human history, from early hominids millions of years ago, to modern humans.[142] The prevalence of caries increased dramatically in the 19th century, as the Industrial Revolution made certain items, such as refined sugar and flour, readily available.[129] The diet of the newly industrialized English working class[129] then became centered on bread, jam, and sweetened tea, greatly increasing both sugar consumption and caries.

Naturalized from Latin into English (a loanword), caries in its English form originated as a mass noun that means "rottenness",[4][143] that is, "decay". When used in that sense, it takes singular verb inflections (just like the word decay does). Thus caries was not traditionally a plural word synonymous with holes or cavities; that is, it was not the plural form of any singular form cary meaning hole or cavity. Nonetheless, the idea that it is such a plural is a reanalysis that naturally occurs to most English speakers, and the reanalyzed sense is common enough to be entered in various dictionaries and to exist in respectable usage. It still shows a hint of its reanalyzed origins in that it remains idiomatically limited to a plurale tantum sensethat is, like scissors or glasses, one speaks of plural caries obligately in the pluralnot of one scissor, glass, or cary. (This is why one can look for a singular count-noun form of dental cary in any of a dozen major medical and general dictionaries and not find it listed.) Many still use it in the traditional sense (mass, singular), which is why they speak of carious lesions rather than just caries when they intend the plural count sense.

Cariology is the study of dental caries.

It is estimated that untreated dental caries results in worldwide productivity losses in the size of about US$27 billion yearly.[144]

Dental caries is uncommon among companion animals.[145]

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JCI – Welcome

Wednesday, December 7th, 2016

Myocardial infarction (MI) results in the generation of dead cells in the infarcted area. These cells are swiftly removed by phagocytes to minimize inflammation and limit expansion of the damaged area. However, the types of cells and molecules responsible for the engulfment of dead cells in the infarcted area remain largely unknown. In this study, we demonstrated that cardiac myofibroblasts, which execute tissue fibrosis by producing extracellular matrix proteins, efficiently engulf dead cells. Furthermore, we identified a population of cardiac myofibroblasts that appears in the heart after MI in humans and mice. We found that these cardiac myofibroblasts secrete milk fat globule-epidermal growth factor 8 (MFG-E8), which promotes apoptotic engulfment, and determined that serum response factor is important for MFG-E8 production in myofibroblasts. Following MFG-E8mediated engulfment of apoptotic cells, myofibroblasts acquired antiinflammatory properties. MFG-E8 deficiency in mice led to the accumulation of unengulfed dead cells after MI, resulting in exacerbated inflammatory responses and a substantial decrease in survival. Moreover, MFG-E8 administration into infarcted hearts restored cardiac function and morphology. MFG-E8producing myofibroblasts mainly originated from resident cardiac fibroblasts and cells that underwent endothelial-mesenchymal transition in the heart. Together, our results reveal previously unrecognized roles of myofibroblasts in regulating apoptotic engulfment and a fundamental importance of these cells in recovery from MI.

Michio Nakaya, Kenji Watari, Mitsuru Tajima, Takeo Nakaya, Shoichi Matsuda, Hiroki Ohara, Hiroaki Nishihara, Hiroshi Yamaguchi, Akiko Hashimoto, Mitsuho Nishida, Akiomi Nagasaka, Yuma Horii, Hiroki Ono, Gentaro Iribe, Ryuji Inoue, Makoto Tsuda, Kazuhide Inoue, Akira Tanaka, Masahiko Kuroda, Shigekazu Nagata, Hitoshi Kurose

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Stem cells help doctors restore womans smile …

Wednesday, November 23rd, 2016

Half of all traumatic injuries to the face result in a loss of teeth and the surrounding tissue and bone that once supported them, which in turn makes these types of injuries very debilitating and difficult to treat. But in a new study published in the latest issue of STEM CELLS Translational Medicine, doctors at the University of Michigan School of Dentistry (UMSoD), Ann Arbor, have found a new way to regenerate a patients jawbone through the use of stem cells.

The procedure, done under local anesthesia, significantly speeds up the healing time relative to that of traditional bone grafting while allowing a patient to experience only a minimal amount of pain.

Part of a larger clinical trial, the findings highlighted in this issue focus on a 45-year-old woman missing seven front teeth plus 75 percent of the bone that once supported them, the result of a blow to her face five years earlier. She was left with severe functional and cosmetic deficiencies, since the missing bone made it impossible for her to have dental implant-based teeth replacements.

Darnell Kaigler, DDS, MS, PhD, an assistant professor of dentistry in the Department of Periodontics and Oral Medicine, was a lead member of the study team. "In small jawbone defects of the mouth created after teeth were extracted, we have placed gelatin sponges populated with stem cells into these areas to successfully grow bone."

Since the sponge material is soft, it does not work in larger areas. Thus, he and his team of researchers decided to try b-tricalcium phosphate (b-TCP) as a scaffold upon which to place the cells instead. "For treating larger jawbone defects, it is important to have a scaffold material that is rigid and more stable to support bone growth," he explained.

They then placed the b-TCP scaffold, which had been seeded with a mixed population of bone marrow-derived autologous stem and progenitor cells 30 minutes prior to treatment at room temperature, into the defective area of the patients mouth during a procedure that requires only local anesthesia. Four months later, 80 percent of her missing jawbone had been regenerated, allowing them to proceed with placing oral implants that supported a dental prosthesis to once again give her a complete set of teeth.

Study team member Sharon Aronovich, DMD, FRCD(C), a clinical assistant professor of dentistry in the Department of Oral and Maxillofacial Surgery at the UMSoD, said, I am very grateful to all the patients and researchers that participated in this study. Thanks to everyone's efforts, we are one step closer to providing patients with a minimally invasive option for implant-based tooth replacement.

As the first report to describe a cell therapy for craniofacial trauma reconstruction, this research serves as the foundation for expanded studies using this approach, said Anthony Atala, M.D., Editor-in-Chief of STEM CELLS Translational Medicine and director of the Wake Forest Institute for Regenerative Medicine.

The article, Optimized Cell Survival and Seeding Efficiency for Craniofacial Tissue Engineering Using Clinical Stem Cell Therapy, can be accessed at http://www.stemcellstm.com.

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Hematology Conferences | Blood Disorder Conferences | USA …

Thursday, October 13th, 2016

9thInternational Conference on Hematology

Date: November 02-04, 2017

Venue: Las Vegas, USA

Hematology 2016 has been designed with many interesting and informative scientific sessions; it includes all possible aspects of Hematology research.

Hematology

Erythrocytesare also known as red blood cells which carry oxygen to the body and collect carbon dioxide from the body by the use of hemoglobin and its life span of 120 days. along the side the leucocytes helps in protecting the healthy cells because the W.B.C (leucocytes) act as the defending cells in protecting the immune system from the foreign cells. Theseleucocytesare multipotent cells in bone marrow and there life span is of 3-4 days where the yellow blood cells are called as thrombocytes they are where small and irregular in shape they have life span of 5-9 days they are mostly seen in mammals they help in clotting of blood which are in fibrin form called as thrombosis these lead to heart stroke, blockage of blood in blood mostly in arms and legs. where C.B.C is known ascomplete blood countis done to know the number of cells in a body these are mainly done by lab technician presently they are been tested by automatic analyzer the high and low amount of cells will lead to many diseases. Decrease of R.B.C in the body these causes of anemia which leads to weakness, feeling of tired, shortness of breath and person will be noticeably pale. Formation of blood cellular components are called as Hematopoiesis and all the cellular blood components are derived from hematopoiesis stem cells in a healthy individual nearly 10111012new blood cells are produced these help in steady peripheral circulation. If there is a increases of R.B.C in the body these causes polycythemia these can be measured through hematocrit level.

Blood Disorders

Hemophilia Ais a genetic deficiency in clottingfactor VIII,which causes increased bleeding and usually affects males. About 70% of the time it is inherited as an X-linked recessive trait, but around 30% of cases arise from spontaneous mutations. Hemophilia B is ablood clottingdisorder caused by amutationof thefactor IXgene, leading to a deficiency of factor IX. It is the second-most common form ofhaemophilia, rarer thanhaemophilia A. It is sometimes calledChristmas disease, named afterStephen Christmas, the first patient described with this disease.In addition, the first report of its identification was published in the Christmas edition of theBritish Medical Journal.Hemophilia C is a mild form of haemophiliaaffecting both sexes. However, it predominantly occurs in Jews ofAshkenazidescent. It is the fourth most common coagulationdisorder aftervon Willebrand's diseaseandhaemophiliaAandB.In theUSAit is thought to affect 1 in 100,000 of the adult population, making it 10% as common as haemophilia A. Idiopathic thrombocytopenic purpura(ITP), also known asimmune thrombocytopenia,primary immune thrombocytopenia,primary immune thrombocytopenic purpuraorautoimmune thrombocytopenic purpura, is defined as isolated low platelet count (thrombocytopenia) with normalbone marrowand the absence of other causes of thrombocytopeniaVon Willebrand diseasesis the most common hereditarycoagulationabnormality described in humans. Platelets also called "thrombocytes" areblood cellswhose function (along with thecoagulation factors) is to stop bleeding by clumping and clogging blood vessel injuries.Platelets have nocell nucleus: Coagulation is highlyconservedthroughout biology; in allmammals, coagulation involves both a cellular (platelet) and aprotein(coagulation factor) component and these are occoured due togenetic blood disorders

Hematologic Malignancies

Lymphatic leukemiawhich effect the white blood cells(w.b.c) they are closely related to the lymphomas and some of them are unitary diseases which related to the adult T cells leukemia these come under the lymphoproliferative disorders. Mostly they involve in the B-cell sub type lymphocytes. The myeloid leukemia is preferred to the granulocyte precursor in the bone marrow and spinal cord and these arises the abnormal growth in the blood from tissues in the bone marrow. They are mainly related to the hematopoietic cells and these sub title into acute and chronic lymphoblastic leukemia. The acute leukemia is that rapidly producing immature blood cells as they are bulk number of cells healthy cells are not produced in bone marrow due this spill over the blood stream which spread to other body parts. Where as in chronic leukemia highly bulid of matured cells are formed but still abnormal white cells are formed these can not be treated immediately mostly seen in older people. The cancer which originate from white blood cells are called as lymphoma and this disorder is mainly seen inHodgkin lymphomathese diseases is treated by radiation and chemotherapy, orhematopoietic stem cell transplantation. The cancer which starts with in the cell are called as Non Hodgkin lymphocytes and these lymphocytes are of lymph nodes. The bone marrow which develops too many white blood cells leads tomultiple myleoma. The further details on malignance are been discussed inHematology oncology conference-2015.

Hematology and immunology

Blood groupsare of ABO type and but at present the Rh blood grouping of 50 well defined antigens in which 5 are more important they are D,C,c,E and e and Rh factors are of Rh positive and Rh negative which refers to the D-antigen. These D-antigen helps in prevention of erythroblast fetalis lacking of Rh antigen it defined as negative and presences of Rh antigen in blood leads to positive these leads to rh incompatibility. The prevention treatment of diseases related to the blood is called as the Hematology. The hematologists conduct works on cancer to. The disorder of immune system leading to hypersensitivity is called asClinical Immunologyand the abnormal growth of an infection are known as Inflammation and the arise of an abnormal immune response to the body or an immune suppression are known as Auto immune disorder. The stem cell therapy is used to treat or prevent a disease or a condition mostly Bone marrow stem cell therapy is seen and recently umbilical cord therapy Stem cell transplantation strategies remains a dangerous procedure with many possible complications; it is reserved for patients with life-threatening diseases.

Blood Transplantation

Theumbilical cordis a conduit between the developingembryoorfetusand theplacenta. The umbilical vein supplies the fetus with nutrient-richbloodfrom theplacenta The hematopoitic bone marrow transplant, the HSC are removed from a large bone of the donor, typically thepelvis, through a largeneedlethat reaches the center of the bone. Acute myeloid leukemia is a cancerof themyeloidline of blood cells, characterized by the rapid growth of abnormalwhite blood cellsthat accumulate in thebone marrowand interfere withthe production of normal blood cells and the Thrombosis is the formation of ablood clot inside ablood vessel, obstructing the flow ofbloodthrough thecirculatory system. TheHemostaticis a process which causes bleeding to stop, meaning to keep blood within a damaged blood vessel this is the first stage of wound healing. Metabolic syndromeis a disorder of energy utilization and storage, diagnosed by a co-occurrence of three out of five of the following medical conditions, obesity,elevated blood pressure,elevated fasting plasma glucose,high serum triglycerides, and lowhigh-density lipoprotein(HDL) levels. Metabolic syndrome increases the risk developingcardiovascular diseaseanddiabetes.

Diagnosis and Treatment

Palliative careis amultidisciplinary approachto specialisedmedical carefor people with seriousillnesses The spleen, similar in structure to a largelymph node, acts as a blood filter. Anticoagulants(antithrombics) are a class of drugs that work to prevent thecoagulation(clotting) of blood. Some anticoagulants are used in medical equipment, such astest tubes ,blood transfusionbags, andrenal dialysisequipment. Anvena cava filteris a type of vascular filter, amedicaldevice that is implanted byinterventional radiologistsor vascular surgeons into theinferior vena cavato presumably prevent life-threateningpulmonary emboliistherapyusingionizing radiation, generally as part of cancer treatmentto control or killmalignantcells. Radiation therapy may be curative in a number of types of cancer if they are localized to one area of the body. The subspecialty ofoncologythat focuses on radiotherapy is calledradiation oncology. Translational research is another term fortranslated researchandtranslational science, Applying knowledge from basic science is a major stumbling block in science, partially due to the compartmentalization within science. Targeted drug delivery is a method of deliveringmedicationto a patient in a manner that increases theconcentrationof the medication in some parts of the body relative to others.

New Drug Development in Haematology

The development of antibiotic resistance in particular stems from the drugs targeting only specific bacterial molecules. Because the drugisso specific, any mutation in these molecules will interfere with or negate its destructive effect, resulting in antibiotic resistance. Known asDrug deliveryConditions treated with combination therapy includetuberculosis,leprosy,cancer,malaria, andHIV/AIDS. One major benefit of combination therapies is that they reduce development ofdrug resistance, since a pathogen or tumor is less likely to have resistance to multiple drugs simultaneously.Artemisinin-based monotherapies for malaria are explicitly discouraged to avoid the problem of developing resistance to the newer treatment. Drug Induced Blood Disorders causes of sickle cell anemia,pale skin non steroids antiinflammatory drugswhich causes ulcers Using drug repositioning, pharmaceutical companies have achieved a number successes, for examplePfizer'sViagrainerectile dysfunctionandCelgene'sthalidomidein severe erythema nodosum leprosum. Smaller companies, including Ore Pharmaceuticals,Biovista, Numedicus,Melior Discoveryand SOM Biotech are also performing drug repositioning on a systematic basis. These companies use a combination of approaches including in silico biology and in vivo/in vitro experimentation to assess a compound and develop and confirm hypotheses concerning its usage for new indications.

Hematology Research

Lymphatic diseasesthis is a type of cancer of the lymphatic system. It can start almost any where in the body. It's believed to be caused by HIV, Epstein-Barr Syndrome, age and family history. Symptoms include weight loss, fever, swollen lymph nodes, night sweats, itchy skin, fatigue, chest pain, coughing and/or trouble swallowing. Thelymphatic systemis part of thecirculatory system, comprising a network oflymphatic vesselsthat carry a clear fluid called lymph directionally towards the heart. The lymphatic system was first described in the seventeenth century independently byOlaus RudbeckandThomas Bartholin. Unlike thecardiovascular systemthe lymphatic system is not a closed system. The human circulatory system processes an average of 20 litres ofbloodper day throughcapillary filtrationwhich removesplasmawhile leaving theblood cells. Roughly 17 litres of the filtered plasma get reabsorbed directly into the blood vessels, while the remaining 3 litres are left behind in theinterstitial fluid. One of the main functions of the lymph system is to provide an accessory return route to the blood for the surplus 3 litres. Lymphatic diseases are ofNon-Hodgkin's Lymphoma, Hodgkins. Thethymusis a specialized primarylymphoidorgan of theimmune system. Within the thymus,T cellsor Tlymphocytesmature. T cells are critical to theadaptive immune system, where the body adapts specifically to foreign invaders.One of the example of lymph node development. Formation oflymph nodeinto the tumor which lead to cancer called oncology.

Various Aspects of Haematology

Pediatric Haematology and Oncologyis an internationalpeer-reviewedmedical journalthat covers all aspects ofpediatrichematologyandoncology. The journal covers immunology, pathology, and pharmacology in relation to blood diseases and cancer in children and shows how basic experimental research can contribute to the understanding of clinical problems. Physicians specialized in hematology are known ashematologistsorhaematologists. Their routine work mainly includes the care and treatment of patients with hematological diseases, although some may also work at the hematology laboratory viewingblood filmsandbone marrowslides under themicroscope, interpreting various hematological test results andblood clotting testresults. In some institutions, hematologists also manage the hematology laboratory. Physicians who work in hematology laboratories, and most commonly manage them, are pathologists specialized in the diagnosis of hematological diseases, referred to as hematopathologistsorhaematopathologists.Experimental Hematologyis apeer-reviewedmedical journalofhematology, which publishesoriginal researcharticles and reviews, as well as the abstracts of the annual proceedings of theSociety for Hematology and Stem Cells and they should be done under theHematology guidlines.

Blood Based Products

Ablood substituteis a substance used to mimic and fulfill some functions ofbiologicalblood. It aims to provide an alternative toblood transfusion, which is transferring blood orblood-based productsfrom one person into another. Thus far, there are no well-acceptedoxygen-carryingblood substitutes, which is the typical objective of ared blood celltransfusion; however, there are widely available non-bloodvolume expandersfor cases where only volume restoration is required. These are helping doctors and surgeons avoid the risks of disease transmission and immune suppression, address the chronic blood donor shortage, and address the concerns of Jehovah's Witnesses and others who have religious objections to receiving transfused blood.Pathogen reductionusing riboflavin and UV lightis a method by which infectiouspathogensinblood for transfusionare inactivated by addingriboflavinand irradiating withUV light. This method reduces the infectious levels of disease-causing agents that may be found in donated blood components, while still maintaining good quality blood components for transfusion. This type of approach to increase blood safety is also known as pathogen inactivation in the industry. Anartificial cellorminimal cellis an engineered particle that mimics one or many functions of abiological cell. The term does not refer to a specific physical entity, but rather to the idea that certain functions or structures of biological cells can be replaced or supplemented with a synthetic entity. Often, artificial cells are biological or polymeric membranes which enclose biologically active materials. As such,nanoparticles,liposomes,polymersomes, microcapsules and a number of other particles have qualified as artificial cells.Manufacturing of semi synthetic products of drugs are known as therapeutic biological products.Anticoagulants(antithrombics) are a class of drugs that work to prevent thecoagulation(clotting) of blood. Such substances occur naturally in leeches and blood-sucking insects.

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2016 Market Research Reports on 5000+ Sectors at …

Friday, September 23rd, 2016

Notes: Sales, means the sales volume of Coal Fired Boiler Revenue, means the sales value of Coal Fired Boiler This report studies sales (consumption) of Coal Fired Boiler in Global market, especially in USA, China, Europe, Japan, India and South

September 2016

$4000

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September 2016

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September 2016

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September 2016

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September 2016

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September 2016

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September 2016

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September 2016

$4000

Notes: Sales, means the sales volume of UAV Payload Revenue, means the sales value of UAV Payload This report studies sales (consumption) of UAV Payload in Global market, especially in USA, China, Europe, Japan, India and Southeast Asia, focuses

September 2016

$4000

Notes: Sales, means the sales volume of UAV Subsystem Revenue, means the sales value of UAV Subsystem This report studies sales (consumption) of UAV Subsystem in Global market, especially in USA, China, Europe, Japan, India and Southeast Asia, f

September 2016

$4000

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September 2016

$4000

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September 2016

$3900

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September 2016

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Notes: Sales, means the sales volume of Arixtra Revenue, means the sales value of Arixtra This report studies sales (consumption) of Arixtra in Europe market, especially in Germany, UK, France, Russia, Italy, Benelux and Spain, focuses on top pl

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September 2016

$3900

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September 2016

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September 2016

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September 2016

$3900

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Stem Cells – Registered Dental Hygienist – RDH Mag

Wednesday, August 31st, 2016

What will they be when they grow out?

by Diane Brucato-Thomas, RDH, EF, BS, FAADH

To be or not to be? That is the question was likely uttered on the stage at the Shakespeare Festival next door to where we sat on a blanket on the Lithia Park grass, in Ashland, Oregon. We were contemplating the backgammon board between us, as leaves sprinkled down, in the crisp autumn air. Apparently, the backgammon board was not all that Jack was contemplating. A similar question left his lips, Diane, when does the will begin?

Uh, well ... uuhhhhh ... Now, there was a question that I had not thought much about. Jack was good at snatching those kinds of questions out of thin air. Sometimes, I thought he used them as a means of distraction if I was winning, so I would make a dumb move. But then, his letters were the same way: How many drops of water does it take to fill a swimming pool? and he would go about figuring it out step by brilliant step in the body of the letter.

I came back to the question at hand and darn near gave away the game. I never was good at multitasking. Jack looked at me. Well? Does a plant have a will when it turns toward the light? Does a protozoa have a will? Does the will begin before or after the brain develops? Does a sperm cell have a will when it starts its journey with such seeming determination? Or does the will begin when the fertilized egg starts to divide? Or is it when the cells differentiate, determining their purpose within the organism? Exactly when does the will begin?

We ended up going round and round on that one, on that breezy fall day in 1984.

Fifteen years before I met this brilliant man, who could conjure scientific facts and philosophical dilemmas on a whim, Jack was diagnosed with Parkinson's disease. He was far from being alone. Parkinson's is a very common neurodegenerative disorder, found to affect 2% of the population over age 65. Actor Michael J. Fox brought Parkinson's more into the limelight when he was diagnosed at a much younger age. It is caused by a progressive degeneration and loss of neurons in the brain that produce dopamine. The symptoms include tremors, abnormally decreased mobility (hypokinesia), and rigidity. I remember Jack holding my arm as his feet would shuffle to a slow start, just to walk from here to there.

Jack died in 1996.

How I would love to have the opportunity to have a deep discussion with Jack today! Parkinson's disease may be among the first diseases to be well-suited to treatment using stem cell transplantation. Methods to induce embryonic stem cells to differentiate into cells with many of the functions of specific dopamine-producing neuron cells have been successfully developed in several laboratories.

Jack would have been fascinated to learn that recently scientists directed mouse embryonic stem cells to differentiate into dopamine-producing neurons and then transplanted them into the brains of a rat model of Parkinson's disease. These stem cell derived neurons reinnervated the brains of the rat Parkinson model, released dopamine, and improved their motor function!

Scientists are now developing a number of strategies in the laboratory for producing dopamine-releasing neurons from human adult stem cells to be transplanted into humans with Parkinson's disease. If the generation of an unlimited supply of dopamine-producing neurons is successful, neurotransplantation may be widely available for Parkinson's patients in the future.

Parkinson's disease is just one of many previously incurable diseases and conditions that may find a cure in stem cell regenerative therapies. Muscular dystrophy, Alzheimer's disease, osteoporosis, diabetes, cardiovascular disease, renal failure, and spinal cord injuries are a few others. Virtually any disease that occurs as a result of damaged, failing or malfunctioning tissues may potentially be cured through regenerative therapies.

Stem cells from one part of the body will be expanded (grown out) and reimplanted to replace an entirely different type of tissue. This type of transplant will totally negate the need for antirejection drugs, since the implant is made of the patient's own body cells.

Most recently, the very first engineered whole organ transplant, using a windpipe made with the patient's own stem cells, was successfully completed by surgeons in Spain. The patient was a 30-year-old woman, whose airways were damaged by tuberculosis and who needed the transplant to save her lung.

The doctors removed a trachea and bronchus from a donor patient who recently died. Using strong chemicals and enzymes, all of the cells were dissolved from the donor trachea, leaving only a fibrous tissue scaffolding made of collagen protein. This structure was used as the framework to repopulate with the woman's own cells. The doctors used two types of the woman's own cells to populate the scaffold cells lining her own windpipe, and very immature bone marrow cells (adult stem cells), which could be encouraged to form the kind of cells that normally surround the trachea.

The repopulated scaffold was rotated in a special bioreactor and after only four days of growth in the lab, the newly-coated donor trachea was ready for transplant. It looked and behaved identically as a normal human donor trachea would. This was cut to fit and replaced the woman's damaged windpipe. Four days after the transplant, the hybrid trachea was almost indistinguishable from the adjacent normal airway. Further, there was no sign of rejection four months later.

The idea of using stem cells from the person's own body to provide undifferentiated mesenchymal cells, not only circumvents the necessity for antirejection medication, it entirely avoids the controversy of using embryonic stem cells. Most exciting for us in the dental field is that undifferentiated mesenchymal cells originated from the cranial ectoneuromesioderm can be found in the pulp of teeth! These stem cells may be used to produce tissues found from the neck up, including nerves, bone, cartilage, and fat.

This discovery prompted Dr. Greg Chotkowski, an oral surgeon whose own son suffers from muscular dystrophy, to look at the plethora of extracted teeth being discarded daily in an entirely new light. Here was a noninvasive source of magical adult stem cells readily available for patients' future access. This realization led Dr. Chotkowski to create StemSave, a company that provides an opportunity for dentists and patients to recover and cryogenically preserve their own or their child's healthy stem cells for future regenerative purposes. This is an incredible gift of life to offer for your child's future health.

One of my favorite clients is a smart and beautiful 30-year-old mother from Brazil. She was showing me a picture of her darling 10-month-old son, when she cited her gratefulness that he was born in good health. She told me that, at the time when she gave birth, she had been reading about stem cell therapies and research being done, and asked the nurses at her hospital about banking the umbilical stem cells. Surprisingly, she was told that it was too difficult and, therefore, was not given the opportunity. Imagine, this woman was thrilled to hear from me that there would be another opportunity when her little boy began to lose his baby teeth.

Deciduous teeth that are just starting to loosen, particularly incisors or canines, with more than one-third of the root structure intact, are perfect candidates for viable pulpal stem cell recovery. The tooth must be free of infection, deep caries, and have an intact blood supply. Mesodens or supernumerary teeth are another ideal source. Extracted permanent teeth, such as wisdom teeth or bicuspids extracted for orthodontic purposes can also be a source for adult stem cells, as long as the teeth are free of infection or pathology, have a complete root, and intact blood supply.

If a client chooses to bank their dental stem cells, they can enroll online at http://www.stemsave.com. Their dentist will then receive a recovery and transport kit in time for the extraction appointment. When the tooth is removed from the oral cavity at 98.6 degrees, the dentist places the tooth or teeth into a vial included in the recovery kit. This is then placed into a thermos containing a patented phase change material that maintains the internal environment of the thermos at room temperature, inducing hypothermia. UPS is dispatched through StemSave for immediate pickup and delivery within 48 hours. This is critical to keep the pulp alive.

When StemSave receives the package, the teeth are removed and disinfected. Then they are cracked open, and the pulp is removed and tested for viability. The dental stem cells are then cryogenically preserved in liquid nitrogen utilizing DMSO as a cryoprotectant. DMSO prevents crystallization damage within the cells, so, even though frozen, the cells remain undamaged in suspended animation at a cryopreservation facility that has been in business for 25 years.

At this point in time, studies involving the applicability of adult stem cells derived from dental pulp has focused on proving the viability of these cells for regenerative applications. Less invasive than extracting stem cells from conventional sources, such as bone marrow and umbilical cords, dental pulp stem cell extraction is an attractive and less expensive alternative.

Jeremy Mao, DDS, PhD, a professor and director of the Tissue Engineering and Regenerative Medicine Laboratory and professor of dentistry at Columbia University, states that, although more clinical research and differentiation studies are needed involving dental stem cells, the future holds a great potential for the use of these cells. Clinical research of the potential for utilizing these cells in the treatment of diseases or conditions involving neural, bone, cartilage, or fat is just beginning.

Dr. Mao's latest announcement states in an abstract that he has turned dental stem cells into pancreatic beta islet cells that produce insulin. Considering the fact that the occurrence of diabetes in the United States is reaching epidemic proportions, this is most exciting!

Since the very beginning of the profession of dental hygiene, dental hygienists were known as specialists in prevention. In recent years, as periodontal diseases have become linked with systemic diseases, dental hygienists are being touted as not only saving teeth, but saving lives! Now, dental hygienists are in the unique position to not only save lives, but actually play a role in improving the quality of and extending lives.

Dental hygienists see almost every client that comes in the door. How many of these adults need an extraction?

How many college kids are having their wisdom teeth extracted? How many teens are getting orthodontic extractions? How many little tykes are anxiously awaiting the tooth fairy? How many new mothers like my client were denied an opportunity to bank their umbilical stem cells? Imagine the fact that encouraging the simple banking of those viable dental stem cells may save and extend their lives in the event of an unfortunate disease or condition occurring. Talk about making a difference!

Now that I've got your attention, stay tuned. The sequel to this article will go into more depth to help you understand the miraculous life-giving science of stem cells and regenerative medicine.

This brings me back to Jack. I found a couple of letters he wrote to my husband. In this one from 1988, Jack relates that his cousin once asked:

You say, And so you are 70 and have your own hell with that Parkinson's disease.' Has the happiness in your life been worth the suffering?

Jack answered:

My whole life, practically, has been a wonderful experience. Somehow I escaped the thistle and thorns. The accent on my existence has been exploring and knowing. I find the times we live in now most fascinating.

I never planned on an immortal existence ... Man and other living things go through a cycle of birth, life, and death. I remember, as a youngster, asking the question: What is the mechanism for gathering together the residue of ashes or bacterial decay and reconstituting it into a loving sentient being? A shriveled shell of 75 or an improved new young model?'

In another letter from December 1981, Jack writes: How often do you pause to marvel at the new things of recent years? ... sending a space craft to Jupiter and having thousands of pictures sent back, putting a man on the moon, releasing nuclear energy, the wonders of electronics ... Who needs (more) miracles? We've got all we need ... and real ones.

These contemplations make me wish Jack were here now. I would gladly give up winning the backgammon game to discuss the amazing potential of stem cell therapy, regenerative medicine, and the hope of life without the debilitating symptoms. What a way to spend the day!

Originally posted here:
Stem Cells - Registered Dental Hygienist - RDH Mag

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Non-Surgical CAL Stem Cell Facelift Enriched MSc Cells

Thursday, August 18th, 2016

The NEW MSc EnrichedStem Cell Face Lift in Thailandis also known as:

Stemcell facelifts are new scientific breakthrough and Thai Medical is proud to offer Genuine CAL Facelift exclusively in Bangkok Thailand. CAL orCell Assisted Lipotransfer utilizes advanced medical technologies of cell regeneration to bring us one step closer to the elusive Fountain of Youth. Thailand is one of the few countries on the planet that allowstherapeutic cellular expansion/growth in a private stem cell lab. Other countries like the US, Australia and UK do not currently allow the expansion of stem cell and expanded/manipulated cells are considered as drug manufacturing thereby banned by the FDA in those countries. Without cell expansion a treatment becomes simple fat grafting that takesfat and stem cells from your stomach and re injects them back into the face withoutany additional expansion. This method is simply not as effective as culturing and expanding stem cell face lift protocol that we currently offer.

Weoffer men and women a fantastic chance to improve the tone,tightness,elasticity and health of their face,hands and feet with our approved cosmetic stem cell treatmentssuch as CAL breast enlargement & CAL stem cell face lift. Our surgical theater/operating room is the only closed system lab in Thailandand have innovated upon and improved theno surgery face Lift to safely and efficiently and restore your youthful appearances naturally by simply using your own fat derived stem cells without the risks of having major facelift operation. Using your own fat stem cells is 100% safe with no chances of any complications or rejection associated with previous embryonic derived stem cells or traditional surgicalface lifts.

Traditional surgical facelifts are usually good to perform once or a maximum of two times in your life. Stem Cell facelifts can be repeated permanently without the added risk of painful surgeries and recovery. By using your own cells from your belly fat we are able to rejuvenate,restore and repair your face, or body into a more youthful, attractive way that is:

For over 25 years now surgeons have used fat grafts from their patients body to restore elasticity and fullness while eliminating wrinkles in the face,neck and hands. Stem Cells was first used for reconstruction purposes in treatments such as the cell assisted lipotransfer or CALbreast augmentation after breast cancer mastectomy or after suffering severe burns to the hands and face.

Many patients are also taking advantage today of regenerative medical therapies as a safe and natural alternative to orthopedic treatments for connecting tissue without the need for any expensive surgery and/or unnatural implants.

100% Safe 100% Natural 100% UnDetectable!

Since those early days countless new and innovative applications are being used today to treat everything thing from stem cell hair restoration,stem cells for macular/optical regeneration, stem cells for lip augmentation,thigh lifts,stem cell butt lifts, stem cells for anti-agingand neck or brow lifts.

We do not use Vaser or SMART Lipo Laser Liposuction to Remove fat and Stem Cells as this usually destroys the stem cells making the treatment ineffective.

Our previous experiences with stem cells comes from a more practical and life-saving treatments for many diseases today such as

Your hands and neck area can also be effectively regenerated using your own msc enriched adipose stem cells. Our Cell face lift results are also very long term and eliminates the need for temporary injection of fillers such as Botox,Restylane ,Juvederm,hyaluronic acids, facial and skin care.

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Non-Surgical CAL Stem Cell Facelift Enriched MSc Cells

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All Things Stem Cell Hematopoietic Stem Cells: A Long …

Thursday, August 18th, 2016

Promising cures for blood-related diseases, such as leukemia and lymphoma, hematopoietic stem cells (HSCs) have been heavily researched for decades. However, like many significant findings in science, their discovery was not made in search for such a cure, but stumbled upon while dealing with another serious medical issue of the time: radiation. While trying to treat people exposed to lethal doses of radiation during World War II, transplants from the spleen and bone marrow were found to rescue these victims (Ford et al., 1956). It was not until later that scientists determined that the HSCs present in these tissues were what was restoring the damaged tissues, observed by performing transplants using lethally irradiated mouse and rat models (Becker et al., 1963). HSCs in humans were further characterized and cultured in the 1980s (Morstyn et al., 1980; Sutherland et al., 1989; Sutherland et al., 1990). The formation of the National Marrow Donor Program during this time also greatly improved the availability of these cells for research. Not only have HSCs been successfully used clinically in humans since the 1950s, but to this day they are still one of the few adult stem cells to be tested for clinical uses.

It is now not only better understood how HSCs from a donor animal can save a lethally irradiated recipient animal, but how HSCs can be used in many other medical applications as well. HSCs are able to give rise to all cells in the hematopoietic system, which includes myeloid elements (i.e. red blood cells, white blood cells, platelets) and the lymphatic system (i.e. T-Cells) (Regenerative Medicine, 2006). Because radiation generally targets rapidly dividing cells, including bone marrow cells and cells in the lymphatic system, HSCs have the ability to replenish the supply of cells most damaged by radiation. While HSCs can be collected from adult bone marrow, some fetal tissues (liver, spleen, thymus), umbilical chords, and peripheral blood, in recent years there has been a great shift towards obtaining HSCs mainly from peripheral blood, using a much simpler and less controversial procedure, though achieving a large enough number of HSCs for transplants is still an obstacle to overcome (Stem Cells, 2001). HSCs are now being used to treat cancers of the hematopoietic system (leukemia and lymphomas), replenish cells lost to high-doses of chemotherapy, and fight against autoimmune diseases, in addition to other medical applications (Regenerative Medicine, 2006).

Hematopoietic stem cells give rise to two major progenitor cell lineages, myeloid and lymphoid progenitors (Regenerative Medicine, 2006).

A rather unique, and not fully understood, characteristic of HSCs is their mobility; HSCs can travel out of the bone marrow and into surrounding tissues, such as peripheral blood, nearby bones, the spleen, and many other tissues. Multiple studies have shown that HSCs transplanted into a donor animal can travel to such areas especially when they are injured. Though the molecular method for the homing mechanisms is still being elucidated, it is thought that an injury causes a loss of cells normally functioning in the hematopoietic system and this loss must quickly be replaced by HSCs, which migrate to where they are needed and then differentiate into the required, lost derivatives (Whetton and Graham, 1999; Regenerative Medicine, 2006; Chamberlain et al., 2007). While HSCs have been clinically studied to a large extent relative to other human stem cells, much work remains to be done to fully understand their molecular interactions.

References

Becker, A. J., McCulloch, E. A, and Till, J. E. Cytological Demonstration of the Clonal Nature of Spleen Colonies Derived from Transplanted Mouse Marrow Cells. Nature. 1963. 197:452-4. View Article

Chamberlain, G., Fox, J., Ashton, B., Middleton, J. Concise Review: Mesenchymal Stem Cells: Their Phenotype, Differentiation Capacity, Immunological Features, and Potential for Homing. Stem Cells. 2007 Nov;25(11):2739-49. Epub 2007 Jul 26. View Article

Ford, C. E., Hamerton, J. L., Barnes, D. W. H., and Loutit, J. F. Cytological identification of radiation-chimeras. Nature. 1956. 177:4524. View Article

Morstyn, G., Nicola, N. A., and Metcalf, D. Purification of hemopoietic progenitor cells from human marrow using a fucose-binding lectin and cell sorting. Blood. 1980. 56:798-805. View Article

Regenerative Medicine. Department of Health and Human Services. Chapter 2: Bone Marrow (Hematopoietic) Stem Cells. August 2006. http://stemcells.nih.gov/info/scireport/2006report.htm

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Can dental stem cell technology make root canals obsolete …

Tuesday, August 16th, 2016

http://www.naturalnews.com/041111_stem_cells_root_canals_dentistry.html

According to The Wall Street Journal, scientists have made advances in restoring tooth pulp, which would negate having to perform root canals. Per the paper:

Several recent studies have demonstrated in animals that procedures involving tooth stem cells appear to regrow the critical, living tooth tissue known as pulp. Treatments that prompt the body to regrow its own tissues and organs are known broadly as regenerative medicine. There is significant interest in figuring out how to implement this knowledge to help the many people with cavities and disease that lead to tooth loss.

Elimination of root canals?

That's important because, according to statistics, half of children in the U.S. have at least one cavity by the time they're 15 years old; in adults, a quarter of those over the age of 65 have lost all their teeth. The Centers for Disease Control and Prevention says that Americans spent $108 billion on dental care in 2010, a figure which includes elective and out-of-pocket care.

Tooth decay occurs when infections or bacteria outstrip a tooth's natural ability to heal or repair itself. If the damage eventually causes too much erosion of the hard, outer enamel shell and penetrates the tooth, eventually the infection can kill off the soft pulp tissue inside, which makes removal of the tooth or a root canal to repair the damage necessary. Pulp is essential in detecting sensation - heat, cold, pressure - and it is where the stem cells ("undifferentiated cells that turn into specialized ones," WSJ reported) that can regenerate tooth tissue are located.

Researchers from all over the world - the U.S., Japan, South Korea and the U.K., among others - have been working to figure out how to make stem cells regenerate pulp. Right now the research is in its early stages, but if a technique can be found "it could mean a reduction or even elimination of the need for painful root canals," the paper said.

Root canals deal with the tooth's infection but they are not a cure-all, per se, WSJ reports:

While the affected tooth remains in the mouth, it is essentially dead, which could impact functions like chewing. That also means no living nerves remain in the tooth to detect further decay or infection. Infection could subsequently spread to surrounding tissue without detection. An estimated 15.1 million root canals are performed in the U.S. annually, according to a 2005-06 survey by the American Dental Association, the most recent data available.

'Public health issue'

"The whole concept of going for pulp regeneration is that you will try and retain a vital tooth, a tooth that is alive," Tony Smith, a professor in oral biology at the University of Birmingham in the U.K., told the paper. "That means the tooth's natural defense mechanisms will still be there."

He adds: "I think we are really just at the opening stages of what is going to be a very exciting time, because we're moving away from traditional root-canal treatments."

Misako Nakashima, DDS, Ph.D., of the National Center for Geriatrics and Gerontology in Obu, Japan, says the dental stem cell research addresses an important "public health issue."

"Dental cavities and inflammation of the surrounding pulp is a challenging public health issue, as tooth decay not only can cause a patient great pain but it also can lead to other serious health issues including heart disease," he told AlphaMed Press.

"Other studies demonstrated the therapeutic effects and safety of mesenchymal stem cells (MSCs) for various diseases. There have been no preclinical reports, however, to support the use of pulp stem cells as a potential treatment for pulpitis in clinical trials," he continued. "We also knew that previous studies involving the spinal cord show that combining G-CSF with stem cells derived from other types of tissues, bone marrow stromal cells, neuronal stem cells and amniotic fluid stem cells had several benefits. So we reasoned that G-CSF might positively impact the pulp stem cells, too."

Though in the early stages, using stem cell therapies to treat dental problems looks promising.

Sources:

http://online.wsj.com

http://www.prweb.com

http://www.ncbi.nlm.nih.gov

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