StemCell Therapy

ABSTRACT

Objectives:Background for use of autologous adipose tissue as a source of adult progenitor (stem) cells for use in Prolotherapy. Present a means of lipoaspiration to harvest adipose-derived mesenchymal stromal cells (AD-MSC) and the stromal vascular fraction (SVF) for use in Prolotherapy and regenerative medical applications by non-plastic surgeons.

Design:Explain the patented super luer-lok and microcannulas for use with the Tulip Medical closed syringe system. A sequential explanation and equipment selection for minimally traumatic lipoaspiration in small volumes is presented.

Results:Thousands of autologous fat grafts (AFG) have proven safe and efficacious for lipoaspiration techniques for large and small structural fat grafting procedures. Addition of platelet-rich plasma (PRP) to AFG has been used in several thousand cases of HD ultrasonic-guided injection Prolotherapy for musculoskeletal purposes in the past 4 years with excellent clinical outcomes.

Conclusions:Use of Tulip closed syringe lipoaspiration system with microcannulas offers a safe and effective means of harvesting small volumes of non-manipulated adipose tissues and its accompanying progenitor cells within the SVF. It offers a simple and effective means to gather undifferentiated cells for use in Prolotherapy and regenerative medical applications. Syringe and microcannulas offer a compact system and practical protocol for non-plastic surgical practitioners.

Journal of Prolotherapy.2011;3(3):680-688.

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For many years, cosmetic-plastic surgeons have recognized the value of low pressure lipoaspiration for successful transplantation of adipose tissue for structural augmentation. In the introductory years (1980-1990) of liposuction techniques, autologous fat grafting (AFG) was considered unpredictable. Once bioengineers discovered the mechanisms by which lipoaspiration worked, the closed syringe system for gentle harvesting and transplantation was developed and patented. Early belief that effective lipoaspiration was directly related to force of vacuum was replaced by understanding, that, introduction of fluid into the fat layers permitted the fat cells and stroma to enter into a suspension. This suspension was easily extracted through use of closed syringes, and provided adipose tissues with reduced damage and improved grafting results.1

As the importance of tumescent fluid distribution was appreciated, more value was placed in extensive pre-tunneling (moving cannula without applying vacuum). This better distributed local solution and enhanced the ability to mobilize the adipose tissues into a suspension, which yielded more successful and predictable AFG. During the late 1990s, surgeons began to include utilization of platelet-rich plasma (PRP) to further enhance the successes and acceptance of the graft tissues, in both large and small volume transfers.2

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