macromolecules
lysis
detergent
chelating agent
EDTA
nuclease
pellet
PCR
primer
thermalcycle
denature
anneal
extend
Taq DNApol
electrophoretic gel
restriction endonuclease
EcoRI
sticky end
blunt end
compatible end
ligation
ligase
plasmid
transformation
competent
electroporation
selectable marker
agarose
vector
band
ethidium bromide
Southern blot
membrane
denaturation
hybridization
washing
probe
stringency
northern blot
western blot
transgene
GMO
transformation
transfection
naked DNA
vector
electroporation
microinjection
vesicles
callus
knock-out
recalcitrant
Agrobacterium
particle bombardment
T-DNA
Ti plasmid
position effects
stem cells
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8.S: Techniques of Molecular Genetics (Summary)
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